Rapid and precise genotyping of transgene zygosity in mice using an allele-specific method

Jianqi Yang; Alison N DeVore; Daniel A Fu; Mackenzie M Spicer; Mengcheng Guo; Samantha G Thompson; Katelin E Ahlers-Dannen; Federica Polato; Andre Nussenzweig; Rory A Fisher

doi:10.26508/lsa.202201729

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Rapid and precise genotyping of transgene zygosity in mice using an allele-specific method

Jianqi Yang, View ORCID ProfileAlison N DeVore, View ORCID ProfileDaniel A Fu, View ORCID ProfileMackenzie M Spicer, Mengcheng Guo, Samantha G Thompson, View ORCID ProfileKatelin E Ahlers-Dannen, Federica Polato, Andre Nussenzweig, View ORCID ProfileRory A Fisher Correspondence email

Jianqi Yang

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing—original draft, Writing—review and editing

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Alison N DeVore

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing—original draft, Writing—review and editing

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ORCID record for Alison N DeVore

Daniel A Fu

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing—review and editing

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ORCID record for Daniel A Fu

Mackenzie M Spicer

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Conceptualization, Formal analysis, Validation

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ORCID record for Mackenzie M Spicer

Mengcheng Guo

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Formal analysis, Validation, Writing—review and editing

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Samantha G Thompson

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Formal analysis, Validation, Writing—review and editing

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Katelin E Ahlers-Dannen

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

Roles: Conceptualization, Investigation, Methodology

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ORCID record for Katelin E Ahlers-Dannen

Federica Polato

3Laboratory of Genome Integrity, National Institutes of Health, Centre for Cancer Research, Bethesda, MD, USA

Roles: Conceptualization, Data curation, Formal analysis, Investigation

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Andre Nussenzweig

3Laboratory of Genome Integrity, National Institutes of Health, Centre for Cancer Research, Bethesda, MD, USA

Roles: Conceptualization, Data curation, Formal analysis, Investigation

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Rory A Fisher

1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA

2Roy J and Lucille A Carver College of Medicine, The University of Iowa, Iowa City, IA, USA

Roles: Conceptualization, Resources, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Methodology, Writing—original draft, Project administration, Writing—review and editing

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ORCID record for Rory A Fisher
For correspondence: rory-fisher@uiowa.edu

Published 10 April 2023. DOI: 10.26508/lsa.202201729

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Allele-specific genotyping transgene zygosity

Jianqi Yang, Alison N DeVore, Daniel A Fu, Mackenzie M Spicer, Mengcheng Guo, Samantha G Thompson, Katelin E Ahlers-Dannen, Federica Polato, Andre Nussenzweig, Rory A Fisher

Life Science Alliance Apr 2023, 6 (6) e202201729; DOI: 10.26508/lsa.202201729

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Allele-specific genotyping transgene zygosity

Jianqi Yang, Alison N DeVore, Daniel A Fu, Mackenzie M Spicer, Mengcheng Guo, Samantha G Thompson, Katelin E Ahlers-Dannen, Federica Polato, Andre Nussenzweig, Rory A Fisher

Life Science Alliance Apr 2023, 6 (6) e202201729; DOI: 10.26508/lsa.202201729

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June 2023

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