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Rapid and precise genotyping of transgene zygosity in mice using an allele-specific method

Jianqi Yang, Alison N DeVore, Daniel A Fu, Mackenzie M Spicer, Mengcheng Guo, Samantha G Thompson, Katelin E Ahlers-Dannen, Federica Polato, Andre Nussenzweig, Rory A Fisher  Correspondence email
Published 10 April 2023. DOI: 10.26508/lsa.202201729

Article Information

vol. 6 no. 6 e202201729
DOI 
https://doi.org/10.26508/lsa.202201729
PubMed 
37037594
Published By 
Life Science Alliance
Online ISSN 
2575-1077
History 
  • Received September 19, 2022
  • Revision received March 22, 2023
  • Accepted March 23, 2023
  • Published online April 10, 2023.
Copyright & Usage 
© 2023 Yang et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

Author Information

  1. Jianqi Yang1, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing—original draft, Writing—review and editing,
  2. Alison N DeVore1, Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing—original draft, Writing—review and editing,
  3. Daniel A Fu1, Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing—review and editing,
  4. Mackenzie M Spicer1, Conceptualization, Formal analysis, Validation,
  5. Mengcheng Guo1, Formal analysis, Validation, Writing—review and editing,
  6. Samantha G Thompson1, Formal analysis, Validation, Writing—review and editing,
  7. Katelin E Ahlers-Dannen1, Conceptualization, Investigation, Methodology,
  8. Federica Polato3, Conceptualization, Data curation, Formal analysis, Investigation,
  9. Andre Nussenzweig3, Conceptualization, Data curation, Formal analysis, Investigation and
  10. Rory A Fisher1,2, Conceptualization, Resources, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Methodology, Writing—original draft, Project administration, Writing—review and editing⇑
  1. 1Departments of Neuroscience and Pharmacology, The University of Iowa, Iowa City, IA, USA
  2. 2Roy J and Lucille A Carver College of Medicine, The University of Iowa, Iowa City, IA, USA
  3. 3Laboratory of Genome Integrity, National Institutes of Health, Centre for Cancer Research, Bethesda, MD, USA
  1. Correspondence: rory-fisher{at}uiowa.edu
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Funding

  • National Institutes of Health

    CA161882
    RA Fisher

  • HHS | National Institutes of Health (NIH)

    AA025919; AA025919-03S1; AA025919-05S1
    RA Fisher
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Allele-specific genotyping transgene zygosity
Jianqi Yang, Alison N DeVore, Daniel A Fu, Mackenzie M Spicer, Mengcheng Guo, Samantha G Thompson, Katelin E Ahlers-Dannen, Federica Polato, Andre Nussenzweig, Rory A Fisher
Life Science Alliance Apr 2023, 6 (6) e202201729; DOI: 10.26508/lsa.202201729
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Volume 6, No. 6
June 2023
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