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Research Articles
Tannins amount determines whether tannase-containing bacteria are probiotic or pathogenic in IBDTA-induced microbial metabolite GA displays dual effects on IBD. GA exerts its function through two ways: influence bacteria penetration and directly affect epithelial cells. Interaction between diet and microbial enzyme is pivotal in affecting IBD.
Dual function of Rab1A in secretion and autophagy: hypervariable domain dependenceGyurkovska et al show that whereas both Rab1A and Rab1B regulate secretion, Rab1A-HVD enables it to localize to autophagosomes and regulate autophagy. This provides a mechanism for a dual functionality of a single Rab in two different cellular pathways.
AurkA nuclear localization is promoted by TPX2 and counteracted by protein degradationThis study shows that TPX2 co-overexpression in non-transformed human cells induces AurkA nuclear accumulation and promotes its non-mitotic oncogenic functions.
BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expressionInteractions between the BRG1 HSA domain and the B-cell CLL/lymphoma 7 proteins facilitate BRG1-mediated chromatin remodeling and induce changes in the gene expression of essential biological pathways.
Activation and inhibition of the C-terminal kinase domain of p90 ribosomal S6 kinasesThe design of a constitutively active form of the C-terminal kinase domain of the p90 ribosomal S6 kinase 2 (RSK2) is described, and this is based on a phosphomimetic mutation and a C-terminal truncation, yielding a high kinase activity.
Single-cell whole-genome sequencing, haplotype analysis in prenatal diagnosis of monogenic diseasescbNIPT by WGS and haplotype analysis predict the inherited genotypes for families with monogenic diseases and provide a potentially better solution for prenatal diagnosis of monogenic diseases.
Segregation of pathways leading to pexophagyPexophagy occurs via distinct NIX- and USP30/NBR1-dependent pathways.
Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancerThe expression of 5-lipoxygenase, the key enzyme in leukotriene biosynthesis, is suppressed in proliferating cancer cells by a PI3K/mTORC-2– and MEK-1/ERK-dependent axis involving E2F1 and b-Myb.
Disruption of polyhomeotic polymerization decreases nucleosome occupancy and alters genome accessibilityDisruption of SAM domain–mediated polymerization of Polycomb Group protein polyhomeotic decreases nucleosome occupancy in Drosophila S2 cells.
Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant speciesCell division is a crucial step in the development of plants, and the present study reports two novel inhibitors of plant cell division, which affect microtubule organization and cytokinesis.
Assessing the impacts of various factors on circular RNA reliabilityThis study systematically assesses the impacts of numerous factors related to circRNA identification, conservation, biogenesis, and function on circRNA reliability, providing a useful guideline and an important resource for selecting high-confidence circRNAs for further investigations.
Regulatory de novo mutations underlying intellectual disabilityThis study provides suggestive evidence that the de novo mutations in fetal brain-specific enhancers play role in the aetiology of intellectual disability.
RAS and PP2A activities converge on epigenetic gene regulationThis study reveals a hidden regulatory layer in epigenetic gene expression in cancer by co-operative phosphoregulation by RAS and tumor suppressor phosphatase PP2A.
Defibrotide impact on the acute GVHD disease incidence in pediatric hematopoietic stem cell transplant recipientsThe prophylactic use of defibrotide in pediatric HSCT recipients significantly reduces the incidence and severity of acute GVHD, with a modification of cytokine pattern, both strongly coherent with the protective drug’s action.
Endemic Burkitt lymphoma avatar mouse models for exploring inter-patient tumor variation and testing targeted therapiesPatient-derived tumor models show promise for evaluating novel cancer treatments that could improve outcomes for African children diagnosed with EBV-associated endemic Burkitt lymphoma.
A multiplexed siRNA screen identifies key kinase signaling networks of brain gliaThis study reports a multiplexed brain glial kinase screen, providing insight into the largely unknown glial intracellular signaling networks controlling neuroinflammation and highlights the therapeutic potential of targeting glial kinases to modulate glial phenotypes and treat neuroinflammatory disorders.
Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endD2 region in CAMSAP3 preferentially bound to expanded microtubule lattices and also induced lattice expansion, explaining the molecular functions of CAMSAP3.
Analysis of context-specific KRAS–effector (sub)complexes in Caco-2 cellsWe used affinity purification of KRAS in different genetic and growth contexts to determine KRAS-mediated complexes, their rewiring in specific contexts, and their link to functional outcomes.
H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in HydraH4K20me1 modification by SETD8 methyltransferase is typically associated with repression. In Hydra, it is associated with activation machinery locally at Wnt-responsive promoters indicating its dual role in transcriptional regulation.
MiR-532-3p inhibited the methylation of SOCS2 to suppress the progression of PC by targeting DNMT3AMiR-532-3p has a low expression in pancreatic cancer, but its overexpression can inhibit the tumor progression via DNMT3A-dependent inhibition of SOCS2 methylation.
Methods
Plasma proteome dynamics of COVID-19 severity learnt by a graph convolutional network of multi-scale topologyWe used a graph convolutional network to model the plasma proteome of 384 COVID-19 patients. The model incorporates clinical data and is able to predict severity. We identified the proteins that shifted most in expression depending on severity.
New algorithms for accurate and efficient de novo genome assembly from long DNA sequencing readsInnovative algorithmic approaches were used to perform assembly of complex genomes across the tree of life, from long DNA sequencing data.
Efficient knock-in method enabling lineage tracing in zebrafishThis study introduces a knock-in method in zebrafish that is cloning-free, preserves the endogenous gene, achieves high germline transmission, and useful for both cell labelling and lineage tracing.
A protocol for single nucleus RNA-seq from frozen skeletal muscleThe authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples.
Corrections
Correction: Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3Chitinase 1 (CHIT1) plays a role in the pathogenesis of pulmonary fibrosis by modulating canonical and noncanonical TGF-β signaling via interaction with TGFBRAP1 and FOXO3. These findings highlight the CHIT1/SMAD7 axis as a potential biomarker and therapeutic target of pulmonary fibrosis.
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