Table of Contents
Research Articles
Protein S-palmitoylation regulates different stages of meiosis in Schizosaccharomyces pombeThe vital role of S-palmitoylation in cell differentiation is demonstrated in the fission yeast by showing that the modification is required at multiple meiotic stages including mating and karyogamy.
Vasopressin V1a receptor and oxytocin receptor regulate murine sperm motility differentlyEpididymal vasopressin V1a receptor is involved in the regulation of sperm motility, and its antagonist may be a therapeutic agent for male-type reproductive dysfunction.
Human adenylyl cyclase 9 is auto-stimulated by its isoform-specific C-terminal domainThe isoform-specific carboxyl-terminal domain stimulates cAMP production by AC9 and also prevents its activation by cell-surface receptors. An interplay between the coiled-coil and the carboxyl-terminal domain of AC9 in regulating cAMP output is also shown.
Structural and functional characterization of TraI from pKM101 reveals basis for DNA processingCharacterization of relaxase TraI from pKM101 highlights large conformational changes upon DNA binding, and unlike previous reports shows similar behavior as its homologs from the R388 and F plasmids.
S417 in the CC3 region of STIM1 is critical for STIM1-Orai1 binding and CRAC channel activationThis study shows that S417 of STIM1 plays an essential role in STIM1 C-terminus–Orai1 interaction and CRAC channel activation. It also presents new findings about 2-APB’s action on CRAC channel, which provides a tool for better understanding of SOCE activation.
Membrane cholesterol regulates inhibition and substrate transport by the glycine transporter, GlyT2Cholesterol binds to the GlyT2 SLC6 transporter, enhancing the effect of bioactive lipid analgesics, providing new therapeutic avenues to treat neuropathic pain and target SLC6 transporters.
Multiple phosphorylation of the Cdc48/p97 cofactor protein Shp1/p47 occurs upon cell stress in budding yeastThis study shows that phosphorylation of the yeast p97/Cdc48 adaptor protein Shp1 is enhanced upon multiple stresses, a process regulated by both Mpk1 kinase and PP1 phosphatase.
Meiotic DNA exchanges in C. elegans are promoted by proximity to the synaptonemal complexExchanges between DNA molecules during meiosis are tightly controlled. This work shows that the synaptonemal complex is instrumental in regulating meiotic exchanges both between the parental chromosomes and between the sister chromatids.
SorCS1 inhibits amyloid-β binding to neurexin and rescues amyloid-β–induced synaptic pathologyThe protein sorting receptor SorCS1 shields the synapse organizer β-neurexins from amyloid-β oligomers (AβOs) to alleviate AβO-induced synaptic pathology.
Lung macrophages utilize unique cathepsin K–dependent phagosomal machinery to degrade intracellular collagenThis study provides a comprehensive map of how peritoneal, lung, and brain tissue environment shapes phagosomal composition, revealing cathepsin K as the main peptidase in the intracellular destruction of collagen, which is mainly active in lung macrophages.
The GET pathway serves to activate Atg32-mediated mitophagy by ER targeting of the Ppg1-Far complexThe GET pathway promotes anchoring of the Ppg1-Far phosphatase complex to the ER membrane, contributing to initiation of Atg32-mediated mitochondria-specific autophagy in budding yeast.
Mature B cells and mesenchymal stem cells control emergency myelopoiesisMature B cells control lymphotoxin-beta receptor signaling in mesenchymal stem cells to promote emergency myelopoiesis.
FAM21 is critical for TLR2/CLEC4E-mediated dendritic cell function against Candida albicansFAM21 is essential for CD11c+ dendritic cell function to activate host immune response against C.albicans infection.
A cluster of broadly neutralizing IgG against BK polyomavirus in a repertoire dominated by IgMFACS sorting of patient B cells with fluorescent BKPyV VLPs, followed by 10xGenomics scRNAseq, generated a large dataset of BKPyV-specific antibody sequences, among which we were able to identify and characterize broadly neutralizing antibodies.
Cardiac Plin5 interacts with SERCA2 and promotes calcium handling and cardiomyocyte contractilityWe show that elevated cardiac Plin5 correlates with up-regulation of cardiac contraction–related processes, unraveling a novel Plin5 interaction with SERCA2 associated with improved calcium handling.
PKCβII activation requires nuclear trafficking for phosphorylation and Mdm2-mediated ubiquitinationNuclear trafficking that accompanies ubiquitination and phosphorylation at critical residues plays essential roles in the activation of PKCβII, a representative member of the conventional PKC isoforms.
The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growthThe FERM domain protein iTAP/Frmd8, which binds to and stabilizes the ADAM17/iRhom sheddase complex at the cell surface, is a regulator of inflammation, epithelial homeostasis, and tumor growth in vivo.
The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performanceThis study adds insights into FTSJ1 tRNA 2′-O-methylation–associated pathologies in humans and Drosophila by identification of novel FTSJ1 targets and neuron morphology defects.
Linear ubiquitination induces NEMO phase separation to activate NF-κB signalingThe E3 ubiquitin ligase HOIP generates linear ubiquitin chains that promote liquid-liquid phase separation of NEMO in vitro, which correlates with the formation of NEMO condensates and IKK complex activation in cells.
A genome-wide CRISPR functional survey of the human phagocytosis molecular machineryThe validation and interpretation of a genome-wide CRISRPko FACS screen via protein–protein interaction data yield a broad view of the molecular network-regulating phagocytosis in human cells.
Multi-level interaction between HIF and AHR transcriptional pathways in kidney carcinomaThis pan-genomic study of crosstalk between HIF and AHR transcription factors reveals both antagonistic and cooperative interactions and highlights their relevance to kidney cancer and its treatment.
Epigenetic signals that direct cell type–specific interferon beta response in mouse cellsPreexisting cell type–specific chromatin features represent an additional regulatory layer for interferon beta response and direct binding of STAT transcription factors and gene induction.
Plant mobile domain proteins ensure Microrchidia 1 expression to fulfill transposon silencingThe plant mobile domain proteins MAIN and MAIL1 control the expression of the epigenetic factor Microrchidia 1, which has been involved in transposable element silencing in Arabidopsis.
Loss of dyskerin facilitates the acquisition of metastatic traits by altering the mevalonate pathwayCholesterol metabolism may be involved in the early steps of tumor dissemination. Epigenetic regulation of cholesterol synthesis and uptake is of great importance in this process and may constitute a vulnerability of the cancer cell that could be exploited pharmacologically.
Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stressThis study reports that NLRP3 is down-regulated in cancers and identifies a novel non-immune function for NLRP3 in maintaining genome integrity through the control of ATM activation in response to DSB.
The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stressThe conserved protein RIF1 stabilizes replication factories and regulates cohesin recruitment in response to replication stress to limit DNA lesions.
Expansion microscopy reveals subdomains in C. elegans germ granulesVisualising C. elegans germ granules using the super resolution technique expansion microscopy against the backdrop of proteome staining reveals granule ultrastructure.
SRSF7 and SRSF3 depend on RNA sequencing motifs and secondary structures to regulate MicroprocessorMicroprocessor cleaves pri-miRNAs to produce miRNAs. SRSF7 and SRSF3 interact with specific RNA elements in pri-miRNAs to modulate the cleavage of Microprocessor, thereby regulating miRNA biogenesis.
Aberrant association of chromatin with nuclear periphery induced by Rif1 leads to mitotic defectOverexpression of Rif1 induces relocation of chromatin to nuclear periphery and mitotic defect, suggesting a role of regulated chromatin–nuclear membrane association in the proper progression of M phase.
The human MRS2 magnesium-binding domain is a regulatory feedback switch for channel activityMagnesium (Mg2+) binding to the human mitochondrial RNA splicing 2 (MRS2) protein channel amino terminal domain disrupts homomeric interactions and inhibits mitochondrial Mg2+ uptake as a negative feedback mechanism.
A novel hypervariable variable number tandem repeat in the dopamine transporter gene (SLC6A3)This VNTR has a heterozygosity value of 0.93, forms G-tetrads, and is in low linkage disequilibrium with surrounding sequence, making it a new site for genetic analysis.
ICOS costimulation is indispensable for the differentiation of T follicular regulatory cellsICOS signaling promotes T follicular regulatory cell differentiation during germinal center reaction safeguarding against autoantibody production.
Genomic insights into antibiotic resistance and mobilome of lactic acid bacteria and bifidobacteriaWhole-genome sequencing is used to discover novel potential mechanisms of antimicrobial resistance and mobile genetic elements in lactic acid bacteria and bifidobacteria from the agro-food chain.
Methods
A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenatesThis novel method can be used in clinical samples to determine CV function in diseases, with the added benefit of being able to use frozen samples in a high-throughput manner and to explore ATP hydrolysis as a drug target for disease treatment.
Resources
A practical comparison of the next-generation sequencing platform and assemblers using yeast genomeThis research analyzes the general and optimal de novo yeast assembly pipeline using the repetitive yeast genome assembly by benchmarking four different sequencing platforms and seven assembly programs.
