Table of Contents
Research Articles
The βI domain promotes active β1 integrin clustering into mature adhesion sitesMana et al show that the extracellular headpiece βI domain is involved in conformationally active β1 integrin clustering, fibrillar adhesion formation, incorporation of soluble fibronectin dimers into fibrillar polymers, and extracellular matrix–dependent endothelial cell migration.
Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesisThe study proved that the loss of Sry circular RNA in testis causes impaired spermatogenesis, and this damage may be related to the decline in the γH2AX level during the meiosis I stage.
Pandemic Vibrio cholerae acquired competitive traits from an environmental Vibrio speciesPandemic-associated Vibrio cholerae interbacterial defense genes were likely acquired through competition with and subsequent gene transfer from the fish pathogen Vibrio anguillarum.
Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegenerationNDR1/2 kinases are essential for efficient endocytosis and protein clearance by autophagy. Neuron-specific dual deletion of NDR1 and NDR2 causes cortical and hippocampal neurodegeneration, implicating these kinases in neuronal protein homeostasis and health.
Synergistic activation of RARβ and RARγ nuclear receptors restores cell specialization during stem cell differentiation by hijacking RARα-controlled programsSynergistic activation of RARβ and RARγ nuclear receptors in mouse stem cells restores cell specialization during neuronal differentiation by hijacking RARα-controlled programs.
The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regenerationPIEZO1, a mechanosensitive ion channel, regulates Rho-GTPase activity and specifically accumulates at the midbody of muscle satellite cells to precisely control cell division for muscle regeneration.
Loss of cell–cell adhesion triggers cell migration through Rac1-dependent ROS generationThis work shows that an increase in intracellular ROS through the Rac1–NOX pathway is a general response upon loss of cell–cell adhesion, which is necessary for triggering cell migration.
Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenesDrosophila pVALIUM10 TRiP RNAi lines cause undesirable knockdown of transgenic reporters and overexpression lines generated with the help of the Gateway cloning technology by targeting attB sites.
A cryo-electron microscopic approach to elucidate protein structures from human brain microsomesUsing cryo-EM to simultaneously identify and solve structures of five enzymes from a raw human brain microsomal lysate, highlighting the potential for facilitating structural-omics at the atomic level.
Simultaneous solving high-resolution structures of various enzymes from human kidney microsomesUsing cryo-EM to simultaneously identify and solve structures of four enzymes from a raw human kidney microsomal lysate that denote its potential in facilitating structural-omics at the atomic level.
Inflammasome is a central player in B cell development and homingWhereas the inflammasome is known to be a key player in innate immunity, this study shows that NLRP3 is essential for egress/mobilization of B cells from the bone marrow to secondary lymphoid organs.
DSCR1 deficiency ameliorates the Aβ pathology of Alzheimer’s disease by enhancing microglial activityThe authors suggested that an important role of Down syndrome critical region 1 (DSCR1) in microglial activation is to provide a potential therapeutic target for microglial Aβ clearance in Alzheimer’s disease.
Revisiting degron motifs in human AURKA required for its targeting by APC/CFZR1AURKA degron motifs are redefined to show that the so-called N-terminal “A-box” is in fact a D-box, and the so-called “D-box” in the C-terminus is not a degron but a motif critical for the active, degradable conformation of AURKA.
Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporterDIRC2 is an H+-driven lysosomal pyridoxine exporter involved in the regulation of cellular pyridoxine storage.
MMS22L-TONSL functions in sister chromatid cohesion in a pathway parallel to DSCC1-RFCA genome-wide CRISPR screen in human DSCC1-knockout cells reveals multiple dependencies, including additive DNA replication defects resulting from POLE3-4 depletion and enhanced sister chromatid cohesion loss following depletion of MMS22L-TONSL.
SLAMF6 compartmentalization enhances T cell functionsAfter stimulation, the T cell co-receptor SLAMF6 colocalizes with the TCR–CD3 complex to enhance T cell activation. Targeting SLAMF6 localization and function with bispecific antibodies is a novel mechanism with potential therapeutic translation.
Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identityLeptospira strains show very large antigenic variation (serovars). Here, the rfb gene cluster is shown to be transferred horizontally within/among Leptospira species, discriminating serovar identity.
Hyperactive immature state and differential CXCR2 expression of neutrophils in severe COVID-19Severe COVID-19 is associated with alterations to the neutrophil compartment with circulating hyperactive immature neutrophils and a maintenance of CXCR2 expression.
Identifying novel regulators of placental development using time-series transcriptome dataNetwork analysis of RNA-seq data generated from mouse placenta at embryonic days 7.5, 8.5, and 9.5 identifies novel regulators of placental development.
Linking medicinal cannabis to autotaxin–lysophosphatidic acid signalingThe authors show that Δ9-trans-tetrahydrocannabinol, the main psychoactive compound in medicinal cannabis, could be considered as a therapeutic agent by inhibiting autotaxin–lysophosphatidic acid production.
Resources
Transcriptome analyses in infertile men reveal germ cell–specific expression and splicing patternsWe uncover extensive germ cell type–dependent transcriptome changes in human spermatogenesis and demonstrate the potential of alternative splicing in the regulation of spermatogenesis.
Corrections
- Correction: Building in vitro models of the brain to understand the role of APOE in Alzheimer’s disease
Human induced pluripotent stem cell (hiPSC)–based models of the brain will be key to unraveling the role of APOE ɛ4 in the interconnected cellular changes underlying Alzheimer’s disease.
- Correction: Single-cell RNA sequencing of human breast tumour-infiltrating immune cells reveals a γδ T-cell subtype associated with good clinical outcome
Single cell sequencing of γδ-T cells from human blood and tumours revealed novel markers of subtypes with distinct effector functions and a subtype that is associated with favourable clinical outcome.
Reviewer Index
- 2022 Reviewer Index
The LSA Editors thank these scientists for sharing their time and expertise while reviewing manuscripts in 2022. We sincerely appreciate their support.
