Table of Contents
Research Articles
Nanoscaled RIM clustering at presynaptic active zones revealed by endogenous taggingThis study introduces endogenously epitope-tagged RIM in Drosophila melanogaster and demonstrates its nanoscale reorganization at active zones during homeostatic plasticity using super-resolution localization microscopy.
Mitochondrial membrane potential acts as a retrograde signal to regulate cell cycle progressionThis study finds that decreases in mitochondrial membrane potential serve as a means of mito-cellular signalling to delay the transition of yeast cells from the G1 to the S phase of the cell cycle.
RB loss sensitizes cells to replication-associated DNA damage after PARP inhibition by trappingAfter PARP inhibition by trapping, cells lacking the RB tumor suppressor experience persistent replication stress and genomic instability that compromise cell viability.
SiR-DNA/SiR–Hoechst-induced chromosome entanglement generates severe anaphase bridges and DNA damageLive-cell imaging-based study calls for caution when using SiR-DNA, as it induces chromosome entanglement that severely impairs sister chromatid segregation in mitosis, consequently causing DNA damage.
Glioblastoma cellular MAP4K1 facilitates tumor growth and disrupts T effector cell infiltrationMAP4K1 acts as a cancer cell-intrinsic driver of tumorigenesis and immune evasion in human gliomas, specifically glioblastoma multiforme, by modulating cytokine–chemokine signaling networks.
Themis controls T cell activation, effector functions, and metabolism of peripheral CD8+ T cellsAcute deletion of Themis in peripheral CD8+ T cells shows Themis controls T cell receptor signaling, effector functions, and metabolism. Signal cascade adaptation occurred with chronic Themis deletion.
Comparative analysis of bioinformatics tools to characterize SARS-CoV-2 subgenomic RNAsThis study compares the performances of three different software in the identification of canonical and noncanonical sgRNAs in amplicon data sequenced with Illumina MiSeq in 250-bp paired ends.
Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranesBiochemical and genetic experiments identify distinct roles of the two domains of Tim50 in the intermembrane space during translocation of proteins into the mitochondria via the TOM and TIM23 complexes.
KCNQ potassium channels modulate Wnt activity in gastro-oesophageal adenocarcinomasKCNQ3, a potassium channel, is frequently genetically altered in gastro-oesophageal adenocarcinomas and found to be significantly associated with WNT and MYC activity, and cellular proliferation.
TRIM25 targets p300 for degradationHere, it is shown that the E3 protein TRIM25 targets the transcriptional co-activator p300 in an ubiquitin-independent manner for proteasomal degradation involving the motor protein dynein.
Causal links of α-thalassemia indices and cardiometabolic traits and diabetes: MR studyCausal MR study links α-thalassemia-related erythrocyte traits to cardiometabolic risks and diabetes susceptibility in Taiwan.
Peptide derived from SLAMF1 prevents TLR4-mediated inflammation in vitro and in vivoSLAMF1-derived peptide P7 inhibits TLR4-mediated signaling and prevents animal death in mice subjected to LPS shock by blocking TIRAP–MyD88 interaction and TRAM recruitment to TLR4.
MUC13 drives cancer aggressiveness and metastasis through the YAP1-dependent pathwayThis study reveals that MUC13 plays a critical role in the metastasis-associated processes via interaction with YAP1 and nuclear translocation of the YAP1-mediated survival complex and a biochemical intervention that interferes with MUC13–YAP1 complex formation can help develop new therapeutics.
Improving lysosomal ferroptosis with NMN administration protects against heart failureNMN supplementation affects lysosome-mediated ferroptosis, leading to improved autophagy, thereby reducing heart failure and prolonging the lifespan in mitochondrial translation-deficient mice.
FAM111A regulates replication origin activation and cell fitnessTight control of FAM111A is essential to maintain cellular homeostasis: FAM111A depletion impairs DNA replication, whereas its unrestrained expression leads to ssDNA formation and cell death.
Functional plasticity in chromosome–microtubule coupling on the evolutionary time scaleWe identify a lethal mutation in a subunit of the Dam1 complex, a chromosome–microtubule coupler in Saccharomyces cerevisiae with point centromeres. However, this mutation is tolerated in organisms with longer regional centromeres, possibly by employing more microtubules to capture each chromosome.
Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infectionThis study reveals JNK/COX-2/HIF-1α axis activation enhances glycolysis, promoting M1 polarization in HIV-1–infected macrophages, offering a novel approach to AIDS-related inflammation.
Identification of FAM53C as a cytosolic-anchoring inhibitory binding protein of the kinase DYRK1AA previously uncharacterized protein, FAM53C, is identified as a binding partner for the kinase DYRK1A involved in numerous neurodevelopmental disorders, such as Down syndrome and autism spectrum disorder. FAM53C anchors the DYRK1A-DCAF7 complex within the cell cytoplasm in a kinase-inactive state.
Fn14 promotes myoblast fusion during regenerative myogenesisGenetic and molecular approaches demonstrate that Fn14 receptor–mediated signaling improves myoblast fusion during skeletal muscle regeneration in a cell-autonomous manner.
Synergistic effect of antagonists to KRas4B/PDE6 molecular complex in pancreatic cancerC14 and P8 combo reveals potent synergy in PDAC models, promising safer, effective therapy than gemcitabine.
SBDSR126T rescues survival of sbds−/− zebrafish in a dose-dependent manner independently of Tp53We created a transgenic line expressing the disease-associated mutation SBDSR126T in the sbds KO zebrafish background. We demonstrated that amount of SBDSR126T is important for development and tp53 pathway activation. Tp53M214K did not rescue neutropenia or survival in the sbds-null zebrafish.
The coenzyme A precursor pantethine enhances antitumor immunity in sarcomaVitB5 level becomes limiting in sarcomas. It is regulated by the pantetheinase activity of VNN1. VNN1 expression in sarcomas is associated with better prognosis and immune infiltration. In mouse, complementation with pantethine a vitB5 precursor boosts antitumor immunity.
Role of ciliopathy protein TMEM107 in eye development: insights from a mouse model and retinal organoidTMEM107 plays a crucial role in early vertebrate eye development and ciliogenesis in the differentiating retina, and its absence leads to eye defects and impaired retinal organoid formation.
Methods
Cell-free chromatin immunoprecipitation to detect molecular pathways in heart transplantationCell-free DNA chromatin immunoprecipitation detects molecular pathways and profiles the sources of tissue injury in heart transplant patients.
Cost-effective DNA methylation profiling by FML-seqA low-cost, high-throughput method for genome-wide DNA methylation profiling based on methylation-specific restriction digestion and sequencing.
scFseCluster: a feature selection-enhanced clustering for single-cell RNA-seq dataThis study presents a novel computational framework scFseCluster for scRNA-seq clustering analysis. Particularly, an excellent feature selection algorithm FSQSSA was encapsulated in the scFseCluster platform, which achieves optimal gene sets for clustering tasks.
Reviews
Regulation of alternative splicing and polyadenylation in neuronsNeurons generate numerous cell-specific isoforms of genes that are expressed more broadly. Here, we review mechanisms that generate neural-specific alternative splicing and 3′UTRs and their biological impacts on the nervous system.
Resources
The highly improved genome of Ixodes scapularis with X and Y pseudochromosomesIxodes scapularis Gulia-Nuss (IscGN) genome assembly and gene set were obtained using DNA from eggs and male and female adult ticks. By combining Hi-C, PacBio HiFi, and Illumina sequencing technologies, we assembled 13 pseudoautosomes and sex chromosomes: X and Y.
Identification of biomarkers, pathways, and therapeutic targets for EGFR–TKI resistance in NSCLCThe exploration of the hub genes related to EGFR–TKI resistance in NSCLC through bioinformatics technologies can improve the understanding of the mechanisms of EGFR–TKI resistance and provide novel insights into therapeutics.
