Table of Contents
Research Articles
Polycomb group ring finger protein 6 suppresses Myc-induced lymphomagenesisMax dimerizes with Mga to form the repressive complex PRC1.6; another PRC1.6 subunit, Pcgf6, suppresses Myc-induced lymphomagenesis but, unexpectedly, does so in a Mga- and PRC1.6-independent manner.
SMN-deficient cells exhibit increased ribosomal DNA damageSMN deficiency leads to elevated ribosomal DNA damage that results in impaired ribosomal RNA synthesis and translation and is accompanied by a motor neuron-specific down-regulation of DDX21 protein.
Tricalbin proteins regulate plasma membrane phospholipid homeostasisThe extended synaptotagmin (E-Syt) family are conserved proteins that function at membrane contacts. This study reveals that yeast E-Syt family members regulate phospholipid homeostasis necessary for plasma membrane integrity.
Single-cell RNA-seq reveals heterogeneity in hiPSC-derived muscle progenitors and E2F family as a key regulator of proliferationThis study identified and characterized four different populations of muscle progenitor cells derived from human induced pluripotent stem cells.
Phospholipids alter activity and stability of mitochondrial membrane-bound ubiquitin ligase MARCH5This study shows that lipids can act as regulators for the ubiquitination process and can control the stability and activity of a membrane-embedded E3 ubiquitin ligase.
The miR-26 family regulates early B cell development and transformationWe show that the activity of the miR-26 family determines early B cell behavior: high miR-26 levels promote cell expansion and block the pre-B to immature B cell transition, whereas a miR-26 reduction limits expansion and enhances pre-B cell differentiation.
The value of genotype-specific reference for transcriptome analyses in barleyWe demonstrate in this study that using a common reference genome may lead to loss of genotype-specific information in the assembled Reference Transcript Dataset (RTD) and the generation of erroneous, incomplete, or misleading transcriptomics analysis results in barley.
Adipose tissue–specific ablation of Ces1d causes metabolic dysregulation in miceCes1d is a crucial enzyme in adipose tissue. Here, we found a high level of Ces1d translocates onto lipid droplets where it digests the lipids to produce unique fatty acids which are key for glucose and lipid metabolism.
Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsThe microtubule-associated proteins Map7D2 and Map7D1, which belong to the MAP7 family, stabilize microtubules through distinct mechanisms for the control of cell motility and neurite outgrowth.
Nutrient sensitive protein O-GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesisMaternal hyperglycemia and elevated O-GlcNAc levels perturb promoter bivalency and lead to transcriptional up-regulation of neurogenic transcription factors during embryonic neurogenesis.
Subgenomic RNA profiling suggests novel mechanism in coronavirus gene regulation and host adaptionCORONATATOR, developed to analyze the subgenomic RNA of coronavirus, was applied on large number of sequencing data sets from coronaviruses, and shed light on viral gene regulation.
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26This study provides molecular insights into RNA target recognition by the TRIM-NHL protein Mei-P26 that functions in Drosophila ovarian germline stem cell maintenance, oogenesis, and spermatogenesis.
Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal driftAnalysis of clonal dynamics of intestinal stem cells supports an accelerated clonal drift upon aging, likely because of reduced adhesion of aged ISCs because of reduced canonical Wnt signaling.
Analyzing efficacy, stability, and safety of AAV-mediated optogenetic hearing restoration in miceThis longitudinal study revealed stable expression of the fast-gating channelrhodopsin f-Chrimson in cochlear neurons of mice over at least 2 yr upon a single postnatal AAV dosing of the cochlea.
Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in Candida albicansStructural characterization of shikimate pathway multi-enzyme Aro1 from opportunistic fungal pathogen Candida albicans provides the first molecular image of all five domains. Only four of these domains are active and individually essential for C. albicans viability.
Methods
Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencingThis article proposed the correctable decoding sequencing technology with conservative theoretical error rate of 0.0009%, and evaluated its robustness by simulation. This technology can provide a powerful new protocol for NGS platforms, enabling accurate identification of rare mutations in medicine.
Resources
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reportersDescription of affordable ($40) open-source platform for real-time in-cell monitoring of luciferase activity.
Systematic identification of ALK substrates by integrated phosphoproteome and interactome analysisIntegrated analysis of the phosphoproteome and interactome of anaplastic lymphoma kinase (ALK)-overexpressing HEK 293 cells revealed 37 ALK substrate candidates, contributing to the improvement of kinase activity prediction.
