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Research Articles
Mammalian splicing divergence is shaped by drift, buffering in trans, and a scaling lawThis study globally investigates the allelic splicing pattern in multiple tissues of an F1 hybrid mouse and reveals the underlying driving forces shaping such tissue-dependent splicing divergence.
Retinoblastoma protein regulates carcinogen susceptibility at heterochromatic cancer driver lociThe retinoblastoma protein regulates mutagenic potential via control of UV-induced DNA lesion acquisition, thus revealing novel mechanisms that contribute to its tumor suppressor capabilities.
New insights into the phosphorylation of the threonine motif of the β1 integrin cytoplasmic domainThis study investigates phosphorylation of and kindlin recruitment by the double-threonine motif in the β1 integrin cytoplasmic tails.
Rbfox1 is required for myofibril development and maintaining fiber type–specific isoform expression in Drosophila musclesRbfox1 is required for the development of both fibrillar and tubular muscle fibers in adult Drosophila and interacts with Bruno1 to define fiber type–specific patterns of alternative splicing.
Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertensionCap analysis of gene expression (CAGE) tag sequencing from left ventricles of a rat model of hypertension identifies genes that use alternative promoters in a disease condition.
Sensitive visualization of SARS-CoV-2 RNA with CoronaFISHWe design an RNA-FISH method to target the plus and minus strands of SARS-CoV-2 RNA, and apply it to image the virus in cell lines, lung tissue, and nasal swabs, by fluorescence and electron microscopy.
Preclinical and randomized phase I studies of plitidepsin in adults hospitalized with COVID-19This proof-of-concept study shows that plitidepsin, a marine peptide that inhibits SARS-CoV-2 replication, has a favorable safety profile and may be an efficacious treatment for patients with COVID-19.
Chl1 helicase controls replication fork progression by regulating dNTP poolsChl1 helicase affects RPA-dependent checkpoint activation after replication fork arrest by ensuring proper dNTP levels, thereby controlling replication fork progression under stress conditions.
Identification of human gene research articles with wrongly identified nucleotide sequencesThis study describes Seek & Blastn analysis of targeted and journal corpora with manual results verification to identify human gene research articles with wrongly identified nucleotide sequence reagents.
Structural basis of Nrd1–Nab3 heterodimerizationThe NMR structure of an Nrd1–Nab3 chimera describes the structural bases of Nrd1/Nab3 heterodimerization. Nrd1 embraces a bundle of helices in Nab3, building a large interface. Key mutations at that interface compromise cell fitness.
Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA bindingCas9 is sumoylated and ubiquitylated in human cells. K848 is the major SUMO2/3 modification site, but multiple lysines are ubiquitylated, precipitating proteasomal degradation. Preventing Cas9 sumoylation by K848 ablation or by pharmacologic means reduces Cas9 half-life and DNA binding ability.
Testing of the therapeutic efficacy and safety of AMPA receptor RNA aptamers in an ALS mouse modelRNA aptamers designed to block AMPA receptors effectively reduce progression of motor dysfunction, improve TDP-43 pathology, and prevent death of motor neurons in AR2 mice, a mouse model of sporadic ALS, without adverse effects.
Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNFPro-inflammatory TNF is a highly gene-selective inhibitor of the gene expression program of tissue repair and wound healing macrophages.
Age-related differences in immune dynamics during SARS-CoV-2 infection in rhesus macaquesIncreased age is a risk factor for severe COVID-19. Multi-omics profiling in rhesus macaques suggests that aging may delay or impair cellular immune responses and the return to immune homeostasis.
Malaria abrogates O’nyong–nyong virus pathologies by restricting virus infection in nonimmune cellsThis study reveals that acute blood-stage malaria promotes resistance to ONNV infection by inducing IFNg-dependent antiviral status in human and murine nonimmune cells.
Epigenetic modifier balances Mapk and Wnt signalling in differentiation of goblet and Paneth cellsThe histone methyltransferase Mll1 controls intestinal secretory cell fate by promoting Wnt-driven Paneth and restricting Mapk-dependent goblet cell differentiation through regulation of Gata4/6 transcription factors
Phenylbutyrate rescues the transport defect of the Sec61α mutations V67G and T185A for reninUsing cellular models of the ADTKD–SEC61A1 causing mutations V67G and T185A, this study reveals specific aberrations of protein transport and calcium homeostasis as a potential pathogenic mechanism.
Nucleotide biosynthesis links glutathione metabolism to ferroptosis sensitivityThe tumor suppressor protein p53 inhibits ferroptosis by reducing the consumption of glutathione in nucleotide biosynthesis.
Topical TMPRSS2 inhibition prevents SARS-CoV-2 infection in differentiated human airway culturesThere is an urgent need to develop novel therapeutic strategies to prevent viral transmission and COVID-19 disease—we show that topical application of camostat, a TMPRSS2 inhibitor, could be a simple, safe, and effective strategy for restricting upper airway infection.
