Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia

Gimano D Amatngalim; Lisa W Rodenburg; Bente L Aalbers; Henriette HM Raeven; Ellen M Aarts; Dounia Sarhane; Sacha Spelier; Juliet W Lefferts; Iris AL Silva; Wilco Nijenhuis; Sacha Vrendenbarg; Evelien Kruisselbrink; Jesse E Brunsveld; Cornelis M van Drunen; Sabine Michel; Karin M de Winter-de Groot; Harry G Heijerman; Lukas C Kapitein; Magarida D Amaral; Cornelis K van der Ent; Jeffrey M Beekman

doi:10.26508/lsa.202101320

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Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia

View ORCID ProfileGimano D Amatngalim Correspondence email, View ORCID ProfileLisa W Rodenburg, Bente L Aalbers, View ORCID ProfileHenriette HM Raeven, Ellen M Aarts, Dounia Sarhane, Sacha Spelier, Juliet W Lefferts, View ORCID ProfileIris AL Silva, View ORCID ProfileWilco Nijenhuis, Sacha Vrendenbarg, Evelien Kruisselbrink, Jesse E Brunsveld, Cornelis M van Drunen, Sabine Michel, Karin M de Winter-de Groot, Harry G Heijerman, Lukas C Kapitein, View ORCID ProfileMagarida D Amaral, Cornelis K van der Ent, Jeffrey M Beekman

Gimano D Amatngalim

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Supervision, Validation, Investigation, Visualization, Methodology, Writing—original draft, review, and editing

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ORCID record for Gimano D Amatngalim
For correspondence: G.D.Amatngalim@umcutrecht.nl

Lisa W Rodenburg

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Writing—review and editing

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Bente L Aalbers

3Department of Pulmonology, University Medical Center Utrecht, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Validation, Writing—review and editing

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Henriette HM Raeven

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Writing—review and editing

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Ellen M Aarts

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Writing—review and editing

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Dounia Sarhane

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Methodology, Writing—review and editing

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Sacha Spelier

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Writing—review and editing

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Juliet W Lefferts

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Writing—review and editing

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Iris AL Silva

4BioISI-Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Lisboa, Portugal

Roles: Conceptualization, Data curation, Writing—review and editing

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Wilco Nijenhuis

5Department of Biology, Cell Biology, Neurobiology and Biophysics, Faculty of Science, Utrecht University, Utrecht, The Netherlands

6Centre for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Visualization, Writing—review and editing

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Sacha Vrendenbarg

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Writing—review and editing

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Evelien Kruisselbrink

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Writing—review and editing

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Jesse E Brunsveld

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Writing—review and editing

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Cornelis M van Drunen

7Department of Otorhinolaryngology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Methodology, Writing—review and editing

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Sabine Michel

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Investigation, Methodology, Project administration, Writing—review and editing

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Karin M de Winter-de Groot

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Investigation, Methodology, Writing—review and editing

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Harry G Heijerman

3Department of Pulmonology, University Medical Center Utrecht, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Supervision, Validation, Investigation, Writing—review and editing

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Lukas C Kapitein

5Department of Biology, Cell Biology, Neurobiology and Biophysics, Faculty of Science, Utrecht University, Utrecht, The Netherlands

6Centre for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Funding acquisition, Validation, Investigation, Methodology, Writing—review and editing

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Magarida D Amaral

4BioISI-Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Lisboa, Portugal

Roles: Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Investigation, Methodology, Writing—review and editing

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Cornelis K van der Ent

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Methodology, Writing—original draft, review, and editing

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Jeffrey M Beekman

1Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, Utrecht, The Netherlands

2Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands

6Centre for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, The Netherlands

Roles: Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Methodology, Writing—original draft, review, and editing

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Published 3 August 2022. DOI: 10.26508/lsa.202101320

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CFTR drug assay with airway organoids

Gimano D Amatngalim, Lisa W Rodenburg, Bente L Aalbers, Henriette HM Raeven, Ellen M Aarts, Dounia Sarhane, Sacha Spelier, Juliet W Lefferts, Iris AL Silva, Wilco Nijenhuis, Sacha Vrendenbarg, Evelien Kruisselbrink, Jesse E Brunsveld, Cornelis M van Drunen, Sabine Michel, Karin M de Winter-de Groot, Harry G Heijerman, Lukas C Kapitein, Magarida D Amaral, Cornelis K van der Ent, Jeffrey M Beekman

Life Science Alliance Aug 2022, 5 (12) e202101320; DOI: 10.26508/lsa.202101320

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CFTR drug assay with airway organoids

Life Science Alliance Aug 2022, 5 (12) e202101320; DOI: 10.26508/lsa.202101320

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