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Correction: CDKL3 promotes osteosarcoma progression by activating Akt/PKB

View ORCID ProfileAina He  Correspondence email, Lanjing Ma, Yujing Huang, Haijiao Zhang, Wei Duan, Zexu Li, View ORCID ProfileTeng Fei, Junqing Yuan, Hao Wu, Liguo Liu, Yueqing Bai, Wentao Dai, Yonggang Wang, Hongtao Li, Yong Sun, Yaling Wang, Chunyan Wang, View ORCID ProfileTing Yuan, Qingcheng Yang, View ORCID ProfileSonghai Tian, Min Dong, Ren Sheng  Correspondence email, View ORCID ProfileDongxi Xiang  Correspondence email
Aina He
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
2Department of Urology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA
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  • ORCID record for Aina He
  • For correspondence: anna_1188@126.com shengren1211@126.com dxiang@bwh.harvard.edu
Lanjing Ma
3College of Life and Health Sciences, Northeastern University, Shenyang, PR China
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Yujing Huang
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Haijiao Zhang
3College of Life and Health Sciences, Northeastern University, Shenyang, PR China
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Wei Duan
4School of Medicine and Centre for Molecular and Medical Research, Deakin University, Waurn Ponds, Australia
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Zexu Li
3College of Life and Health Sciences, Northeastern University, Shenyang, PR China
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Teng Fei
3College of Life and Health Sciences, Northeastern University, Shenyang, PR China
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Junqing Yuan
5Department of Pathology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Hao Wu
6Department of Vascular Biology, Boston Children’s Hospital, Boston, MA, USA
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Liguo Liu
7Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China
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Yueqing Bai
5Department of Pathology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Wentao Dai
8Shanghai Center for Bioinformation Technology and Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai Industrial Technology Institute, Shanghai, PR China
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Yonggang Wang
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Hongtao Li
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Yong Sun
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Yaling Wang
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Chunyan Wang
1Department of Oncology, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Ting Yuan
9Department of Orthopedics, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Qingcheng Yang
9Department of Orthopedics, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai, PR China
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Songhai Tian
2Department of Urology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA
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Min Dong
2Department of Urology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA
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Ren Sheng
3College of Life and Health Sciences, Northeastern University, Shenyang, PR China
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  • For correspondence: anna_1188@126.com shengren1211@126.com dxiang@bwh.harvard.edu
Dongxi Xiang
10Division of Genetics, Department of Medicine, Brigham and Women’s Hospital, Boston, MA, USA
11Department of Medicine, Harvard Medical School, Boston, MA, USA
12Shanghai Research Center of Biliary Tract Disease Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China
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  • For correspondence: anna_1188@126.com shengren1211@126.com dxiang@bwh.harvard.edu
Published 21 October 2022. DOI: 10.26508/lsa.202201656
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Article: He A, Ma L, Huang Y, Zhang H, Duan W, Li Z, Fei T, Yuan J, Wu H, Liu L, Bai Y, Dai W, Wang Y, Li H, Sun Y, Wang Y, Wang C, Yuan T, Yang Q, Tian S, Dong M, Sheng R, Xiang D (2020 Mar 31) CDKL3 promotes osteosarcoma progression by activating Akt/PKB. Life Sci Alliance 3(5): e202000648. doi: 10.26508/lsa.202000648. PMID: 32234750.

In the published article, there were three errors:

1. We designed the primers for PDX1 using GenBank entry AF049893.1. This sequence was entered in 1997 as human Pdx1, but it is actually rat Pdx1. We were unaware of this error and observed a negligible effect on PDX1, so we removed PDX1 from Fig 5B. This does not have any effect on the other conclusions in Fig 5B.

Figure 5.
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Figure 5.

(B) The expression patterns of mTORC1 and FoxO downstream target genes confirm that CDKL3 regulates both pathways at the transcription level. Cells were cultured under the regular growth condition.

2. In Fig 6C, lower row, patient (a) the exact CDKL3-staining image was placed back.

Figure 6.
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Figure 6.

(C) Representative immunohistochemistry (IHC) images of OS biopsies with different levels of CDKL3 expression on an OS microarray containing 152 primary OS tissues samples. Scale bar = 500 μm.

3. On page 10, “The insert with triple-HA tag at their N termini (with GSGSGSEF as linker) was further cloned to pcDNA3.1 vector via Gibson Assembly (2621; New England Biolabs) and sequenced using primers 5′-GGGCTGATAAAGAAGGCAGAAAATT;

5′-TGCCTTGGCCTCCCAAAGTGCA.” The correct primers should read as follows: 5′-CGCAAATGGGCGGTAGGCGTG; 5′-GCTGGGGTAGTTCTTCCTCAAGTTC.

The conclusions of this article remain unchanged.

  • Received September 14, 2022.
  • Accepted September 21, 2022.
  • © 2022 He et al.
Creative Commons logoCreative Commons logohttps://creativecommons.org/licenses/by/4.0/

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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CDKL3 promotes osteosarcoma via Akt/PKB
Aina He, Lanjing Ma, Yujing Huang, Haijiao Zhang, Wei Duan, Zexu Li, Teng Fei, Junqing Yuan, Hao Wu, Liguo Liu, Yueqing Bai, Wentao Dai, Yonggang Wang, Hongtao Li, Yong Sun, Yaling Wang, Chunyan Wang, Ting Yuan, Qingcheng Yang, Songhai Tian, Min Dong, Ren Sheng, Dongxi Xiang
Life Science Alliance Oct 2022, 5 (11) e202201656; DOI: 10.26508/lsa.202201656

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CDKL3 promotes osteosarcoma via Akt/PKB
Aina He, Lanjing Ma, Yujing Huang, Haijiao Zhang, Wei Duan, Zexu Li, Teng Fei, Junqing Yuan, Hao Wu, Liguo Liu, Yueqing Bai, Wentao Dai, Yonggang Wang, Hongtao Li, Yong Sun, Yaling Wang, Chunyan Wang, Ting Yuan, Qingcheng Yang, Songhai Tian, Min Dong, Ren Sheng, Dongxi Xiang
Life Science Alliance Oct 2022, 5 (11) e202201656; DOI: 10.26508/lsa.202201656
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Volume 5, No. 11
November 2022
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  • He, A., Ma, L., Huang, Y., Zhang, H., Duan, W., Li, Z., Fei, T., Yuan, J., Wu, H., Liu, L., Bai, Y., Dai, W., Wang, Y., Li, H., Sun, Y., Wang, Y., Wang, C., Yuan, T., Yang, Q., Tian, S., Dong, M., Sheng, R., & Xiang, D. (2020). CDKL3 promotes osteosarcoma progression by activating Akt/PKB. Life Science Alliance, 3(5), e202000648. Accessed January 27, 2023. https://doi.org/10.26508/lsa.202000648.

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