Article: He A, Ma L, Huang Y, Zhang H, Duan W, Li Z, Fei T, Yuan J, Wu H, Liu L, Bai Y, Dai W, Wang Y, Li H, Sun Y, Wang Y, Wang C, Yuan T, Yang Q, Tian S, Dong M, Sheng R, Xiang D (2020 Mar 31) CDKL3 promotes osteosarcoma progression by activating Akt/PKB. Life Sci Alliance 3(5): e202000648. doi: 10.26508/lsa.202000648. PMID: 32234750.
In the published article, there were three errors:
1. We designed the primers for PDX1 using GenBank entry AF049893.1. This sequence was entered in 1997 as human Pdx1, but it is actually rat Pdx1. We were unaware of this error and observed a negligible effect on PDX1, so we removed PDX1 from Fig 5B. This does not have any effect on the other conclusions in Fig 5B.
(B) The expression patterns of mTORC1 and FoxO downstream target genes confirm that CDKL3 regulates both pathways at the transcription level. Cells were cultured under the regular growth condition.
2. In Fig 6C, lower row, patient (a) the exact CDKL3-staining image was placed back.
(C) Representative immunohistochemistry (IHC) images of OS biopsies with different levels of CDKL3 expression on an OS microarray containing 152 primary OS tissues samples. Scale bar = 500 μm.
3. On page 10, “The insert with triple-HA tag at their N termini (with GSGSGSEF as linker) was further cloned to pcDNA3.1 vector via Gibson Assembly (2621; New England Biolabs) and sequenced using primers 5′-GGGCTGATAAAGAAGGCAGAAAATT;
5′-TGCCTTGGCCTCCCAAAGTGCA.” The correct primers should read as follows: 5′-CGCAAATGGGCGGTAGGCGTG; 5′-GCTGGGGTAGTTCTTCCTCAAGTTC.
The conclusions of this article remain unchanged.
- Received September 14, 2022.
- Accepted September 21, 2022.
- © 2022 He et al.
https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
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