Table of Contents
Research Articles
Transmembrane protease serine 2 (TMPRSS2) rs75603675, comorbidity, and sex are the primary predictors of COVID-19 severityThe TMPRSS2 rs75603675 genotype (OR = 0.586), dyslipidemia (OR = 2.289), sex (OR = 0.586), and the Charlson Comorbidity Index (OR = 1.126) were identified as the main predictors of COVID-19 severity in 817 patients who attended Hospital Universitario de La Princesa during March and April 2020.
Evolutionary association of receptor-wide amino acids with G protein–coupling selectivity in aminergic GPCRsEvolutionary analyses of aminergic G protein–coupled receptors reveal receptor-wide potential determinants of G protein–coupling selectivity.
MET∆14 promotes a ligand-dependent, AKT-driven invasive growthThe MET oncogene’s most frequent mutation is exon14 deletion. The resulting MET∆14 kinase is not constitutively active but requires HGF. The response to the ligand is stronger and long-lasting, protecting cancer cells from apoptosis and driving invasive growth.
Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradationThis study describes an activity-dependent mechanism for transporting ESCRT-0 protein Hrs to synaptic vesicle (SV) pools, facilitating SV protein degradation in response to increased neuronal firing.
VDAC1 negatively regulates melanogenesis through the Ca2+-calcineurin-CRTC1-MITF pathwayThis study revealed an important and novel role of mitochondrial VDAC1 in regulating melanogenesis in resting melanocytes through a Ca2+-regulated pathway that is independent of the alpha-MSH/UVB pathway.
ERK-Smurf1-RhoA signaling is critical for TGFβ-drived EMT and tumor metastasisThe research uncovers a non-canonical role of ERK in TGF-beta-induced EMT, revealing ERK-mediated phosphorylation of Smurf1 is required for its sufficient binding to RhoA and the subsequent RhoA turnover.
The intracellular pathogen Francisella tularensis escapes from adaptive immunity by metabolic adaptationThis study shows that metabolic adaptation allows the intracellular bacterial pathogen Francisella tularensis to escape recognition by the host adaptive immunity.
Autoreactive CD8 T cells in NOD mice exhibit phenotypic heterogeneity but restricted TCR gene usagePaired scRNA-seq and scTCR-seq reveals that diabetogenic CD8 T cells in the islets and spleens of NOD mice exhibit phenotypic and clonal heterogeneity despite restricted TCR gene usage. Expression of certain TCR genes correlates with clonal proliferation and effector phenotype.
LncRNA RUS shapes the gene expression program towards neurogenesisThe chromatin-associated lncRNA RUS binds in the vicinity to neural differentiation-associated genes and regulates them in a context-dependent manner to enable proper neuron development.
Estimating intraclonal heterogeneity and subpopulation changes from bulk expression profiles in CMapPremnas is a computational framework that provides a new perspective to interpret perturbational data in LINC L1000 CMap by learning an ad hoc subpopulation representation from scRNA-seq and performing the digital cytometry to estimate the abundance of undetermined subpopulations.
TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathyTMBIM5 deficiency reduces mitochondrial K+/H+ exchange. Mutation of the channel pore in mice destabilizes the protein and results in increased embryonic lethality and a skeletal myopathy.
IgM-enriched immunoglobulin improves colistin efficacy in a pneumonia model by Pseudomonas aeruginosaUsing polyclonal IgM-enriched immunoglobulin (IgM-IG) as adjuvant therapy to colistin appears useful in the treatment of pneumonia caused by multidrug-resistant strains of P. aeruginosa.
Neuropeptide signalling shapes feeding and reproductive behaviours in male Caenorhabditis elegansLURY-1 peptides are expressed in distinct cells in different sexes and have sex-specific effects on feeding and mating, providing further evidence for the role of neuromodulators in sexual dimorphism.
chromMAGMA: regulatory element-centric interrogation of risk variantschromMAGMA, a pipeline that prioritizes candidate risk regulatory elements and target genes, reveals novel risk-associated genes when applied to epithelial ovarian cancer.
The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delaysChromosome structuring and condensation is one of the main features of mitosis. Here, Matos-Perdomo et al show how the nuclear envelope reshapes around the vacuole to give rise to the outstanding ribosomal DNA loop in budding yeast.
Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchymaNeutrophils accumulate at the epineurium in Wallerian degeneration (WD) and inhibit macrophage infiltration from the epineurium into the parenchyma by releasing neutrophil extracellular traps, resulting in the delay of repair processes in WD.
Mitotic H3K9ac is controlled by phase-specific activity of HDAC2, HDAC3, and SIRT1Combination of immunofluorescence, Western blot, and ChIP-seq revealed the interplay between HDAC2, HDAC3, and SIRT1 in H3K9 deacetylation during mitosis of mammalian cells.
Phosphorylation of RGS regulates MAP kinase localization and promotes completion of cytokinesisPhosphorylation of the RGS Sst2 alters its subcellular distribution, MAPK localization, and interaction with Kel1, which promotes coordination of polarized growth with completion of cytokinesis.
Binding stoichiometry and structural model of the HIV-1 Rev/importin β complexBiochemical, biophysical, and docking studies provide insights into how the HIV-1 regulatory protein Rev is recognized by the nuclear import factor importin beta.
Proteasomal down-regulation of the proapoptotic MST2 pathway contributes to BRAF inhibitor resistance in melanomaThe loss of MST2 pathway protein expression in BRAF inhibitor resistant melanoma cells is due to ubiquitination and subsequent proteasomal degradation and prevents MST2-mediated apoptosis.
Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changesThe meiotic DNA break response is shown to involve a large number of phosphorylation changes and a clearly detectable transcriptional response. By contrast, the meiotic proteome remains remarkably unchanged in response to breaks.
HIV-1 infection enhances innate function and TLR7 expression in female plasmacytoid dendritic cellsThis study shows that the TLR7-driven innate function of pDCs is increased in HIV/ART women and is associated with enhanced expression of the TLR7 locus from both X chromosomes.
GhRabA4c coordinates cell elongation via regulating actin filament–dependent vesicle transportGhRabA4c is required for cotton fiber cell elongation via functioning in actin filament assembly and bundling, vesicle transport, and deposition of multiple cell wall components.
Regulation of Liprin-α phase separation by CASK is disrupted by a mutation in its CaM kinase domainMutations in the human CASK gene cause a neurodevelopmental disorder; we show that CASK regulates condensate formation of Liprin-alpha 2 and that patient mutations in the CaM kinase domain interfere with Liprin binding and regulation of condensate formation.
Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar systemElectron tomography reveals four classes of cytopathic vesicles-II (CPV-II) stemming from the host Golgi apparatus after Venezuelan equine encephalitis virus infection.
Methods
Workflow for high-dimensional flow cytometry analysis of T cells from tumor metastasesWe describe an improved method for the high-dimensional flow cytometry analysis of conventional and rare unconventional T cells infiltrating tumor liver metastases.
Resources
PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous systemPRMT1 regulates MHC-associated microglia cluster during de/remyelination.
Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cellsSingle-cell RNA sequencing of porcine ileal lymphocytes reveals similarities to human cells and discovery of porcine intestinal innate lymphoid cells.
Corrections
- Correction: Transmembrane protease serine 2 (TMPRSS2) rs75603675, comorbidity and sex are the primary predictors of Covid-19 severity
We identified an error in the abstract of the article: TPMRSS2 rs75603675 OR is incorrectly indicated. It should read (OR = 2.140) instead of (OR = 0.586).
