Table of Contents
Research Articles
Interplay between MPIase, YidC, and PMF during Sec-independent insertion of membrane proteinsCharges in the N-terminal region and the synthesis level of membrane proteins with N-out topology determine YidC and PMF dependencies with the interplay between glycolipid MPIase, YidC, and PMF.
Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlineA TPP1 mutation known to cause telomere shortening and bone marrow failure in humans recapitulates telomere loss but results in severe germline defects in mice without impacting murine hematopoiesis.
HERVH-derived lncRNAs negatively regulate chromatin targeting and remodeling mediated by CHD7Mutations in CHD7 are diagnostic for human CHARGE syndrome. RNAs expressed from the HERVH repeats modulate CHD7 function providing one mechanism for regulation of differentiation of pluripotent cells.
PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islandsPROSER1 promotes the interaction between TET2 and the glycosyltransferase OGT to regulate TET2 O-GlcNAcylation and stability on genomic elements that depend on the activity of the MLL3/4 complexes.
Redundant roles of EGFR ligands in the ERK activation waves during collective cell migrationBy knocking out all four EGFR ligands expressed in MDCK cells, this study shows the redundant and specific roles of each EGFR ligand in the ERK activation waves during collective cell migration.
KIF13B-mediated VEGFR2 trafficking is essential for vascular leakage and metastasis in vivoThe cancer cells secrete VEGF, which induces vascular leakage and metastasis. Inhibition of VEGFR2 trafficking to the cell surface prevents receiving VEGF, vascular leakage, and cancer metastasis.
Single-domain antibody screening by isPLA-seqThis study describes a high-sensitive, high-throughput single-domain antibody library screening approach, which is applicable for any given interested protein at single-cell resolution by isPLA-seq.
Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumoniaA novel mouse model of induced-usual interstitial pneumonia shows bimodal fibrosis with honeycombing, similar to chronic lung disease seen with idiopathic pulmonary fibrosis.
Microbiota regulates the turnover kinetics of gut macrophages in health and inflammationMaintenance of the gut lamina propria–resident macrophage cell pool requires monocyte input during adulthood. Here, Ruedl and colleagues demonstrate that the intestinal microbiome positively influences the replenishment of tissue-resident macrophages under both steady-state and inflammatory conditions.
Structure of Aedes aegypti procarboxypeptidase B1 and its binding with Dengue virus for controlling infectionUsing high-resolution structure, we have characterized the substrate specificity of Aedes aegypti procarboxypeptidase B1 and provided mechanistic insights into the binding and inhibition of DENV
Structural basis of membrane recognition of Toxoplasma gondii vacuole by Irgb6Upon Toxoplasma gondii infection, Irgb6 is recruited to the parasitophorous vacuole membrane (PVM) where Irgb6 disrupts it. We solved the atomic structures of Irgb6 in two distinct nucleotide states, revealing a unique PVM-binding interface sensitive to GTPase cycling.
Molecular and genetic dissection of recursive splicingRecursive splicing is an unusual form of stepwise mRNA processing involving exons with functional splice donors at their 5′ ends. We use molecular and genetic assays to show three parameters that influence mRNA isoform outcomes during recursive splicing in Drosophila.
Integrated stress response restricts macrophage necroptosisStress inhibits necroptosis in a PERK-dependent manner via reduced RIPK1-RIPK3-MLKL signaling, showing an integral mechanistic connection between stress responses and programmed cell death.
Resources
Elevation in sphingolipid upon SARS-CoV-2 infection: possible implications for COVID-19 pathologySARS-CoV-2 infection alters the levels of sphingolipids early post infection. This phenomenon is reflected by increased levels of sphingolipids, including gangliosides, in infected cells, as well as in serum in a SARS-CoV-2 murine model.
