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Research Articles
A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζAs an integral scaffolding component of DNA polymerase (Pol) zeta, Pol lambda adapts Pol zeta–dependent translesion synthesis to operate in a predominantly error-free manner in human cells.
A novel canis lupus familiaris reference genome improves variant resolution for use in breed-specific GWASThis paper describes the economical optimization of DNA extraction, short and long read sequencing, and bioinformatic assembly, which in turn improves mapping and variant resolution of short-read sequenced, same-breed dogs for association studies. This methodology is applicable for any investigators planning a genome-wide association study.
Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cellsThe study uses a reaction probe to target insulin-expressing pancreatic β-cells and β-like cells derived from pluripotent stem cells by harnessing high intracellular Zn(II) concentration.
Functional interaction between macrophages and hepatocytes dictate the outcome of liver fibrosisXie et al demonstrate opposite outcomes on liver fibrosis due to deletion of c-Jun in hepatocytes alone or along with Kupffer cells, highlighting the functional interaction between these cell types in regulating the fibrotic process, dictated by c-Jun.
TrkA-mediated endocytosis of p75-CTF prevents cholinergic neuron death upon γ-secretase inhibitionThe findings shed light into the adverse effects of GSIs observed in the Alzheimer’s field and explain, at least in part, the unexpected worsening in cognition observed in the semagacestat Phase 3 trial.
Lipocalin 13 enhances insulin secretion but is dispensable for systemic metabolic controlThorough preclinical evaluation reveals a negligible role of lipocalin 13 in systemic glucose and lipid metabolism.
1,6-hexanediol rapidly immobilizes and condenses chromatin in living human cellsAlcohol, 1,6-hexanediol, is widely used for melting liquid droplets formed by liquid0–liquid phase separation; however, it can also seriously affect cells by immobilizing and condensing chromatin.
Structural comparison of GLUT1 to GLUT3 reveal transport regulation mechanism in sugar porter familyA new structure of GLUT1, compared with GLUT3, can explain substrate affinity differences, and provide a functional rationale for key structural motifs that define the universal Sugar Porter family.
TrkB signaling regulates the cold-shock protein RBM3-mediated neuroprotectionThe neuroprotective effects of the cold-shock protein RBM3 have broad appeal for therapy. RBM3 induction through cooling occurs through a non-canonical pattern of TrkB signaling. The pathway can now be targeted pharmacologically for RBM3-mediated neuroprotection without the need for cooling.
CDCP1 promotes compensatory renal growth by integrating Src and Met signalingCDCP1 promotes HGF-induced compensatory renal growth by focally and temporally integrating Src and Met-STAT3 signaling in lipid rafts.
Structural basis of nucleosomal histone H4 lysine 20 methylation by SET8 methyltransferaseCryo-EM structures of the human SET8–nucleosome complexes reveal the mechanism by which the SET8 methyltransferase binds the nucleosome and specifically recognizes the histone H4 lysine-20 residue.
A conserved PI(4,5)P2–binding domain is critical for immune regulatory function of DOCK8This study uncovers a critical role of DOCK8 in coupling PI(4,5)P2 signaling with Cdc42 activation through its DHR-1 domain during interstitial leukocyte migration.
SARS-CoV-2 infection induces protective immunity and limits transmission in Syrian hamstersUsing an aged Syrian hamster model, which develops severe morbidity upon SARS-CoV-2 infection including death, we demonstrated that the immunity induced by a natural infection not only protects hamsters from reinfection but also significantly limits transmission via close contact.
Short-term IL-15 priming leaves a long-lasting signalling imprint in mouse NK cells independently of a metabolic switchNK cell reactivity is dynamically regulated by IL-15, and NK cells do not need more than a few minutes of exposure to remember the cytokine for several hours.
Concomitant gain and loss of function pathomechanisms in C9ORF72 amyotrophic lateral sclerosisAxonal trafficking deficits and neurodegeneration in C9ORF72 motoneurons are mediated by GOF and LOF mechanisms with RNA foci and DPRs as upstream events, whereas DNA damage appears downstream.
