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Research Articles
- Paneth cell–derived growth factors support tumorigenesis in the small intestine
Paneth cells, known for their production of antimicrobial peptides and growth factors in the gut epithelium, are found to play a key role in intestinal tumor formation through secretion of Wnt3.
- HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails
Ciliary accumulation of G protein–coupled receptors HTR6 and SSTR3 depends on redundant ciliary targeting sequences acting via ciliary trafficking adapters TULP3 and RABL2.
- Modulation of Aub–TDRD interactions elucidates piRNA amplification and germplasm formation
N-terminal Aub arginines are dispensable for primary piRNA biogenesis but essential for amplification; their symmetric dimethylation is required for germline inheritance but not for piRNA amplification.
- The RNA-binding profile of the splicing factor SRSF6 in immortalized human pancreatic β-cells
The RNA-binding protein SRSF6 recognizes a purine-rich consensus motif consisting of GAA triplets, and its downregulation in human pancreatic β-cells affects alternative splicing in a position-dependent manner.
- The uncharacterized protein FAM47E interacts with PRMT5 and regulates its functions
The uncharacterized protein FAM47E interacts with and stabilizes the protein arginine methyltransferase PRMT5, regulating its epigenetic functions thereby modulating target gene expression.
- Genetic and stochastic influences upon tumor formation and tumor types in Li-Fraumeni mouse models
This study used mice heterozygous for Tp53 mutations with different genetic backgrounds to investigate the genetic or stochastic factors that modify the penetrance of tumor formation by Tp53 mutation.
- The Paf1 complex positively regulates enhancer activity in mouse embryonic stem cells
Using ChIP-seq and functional genomic analyses, the study shows that the Paf1 complex occupies transcriptional enhancers and positively regulates their activity.
- Tissue-selective alternate promoters guide NLRP6 expression
The NLRP6 innate immune sensor is regulated by tissue-selective alternate promoters that facilitate translational gene silencing outside of the intestinal epithelium in both humans and mice.
- Cyclin A2 localises in the cytoplasm at the S/G2 transition to activate PLK1
Main mitotic kinases as PLK1 are activated at the S/G2 transition. A change in Cyclin A2 localisation at the S/G2 transition enables activation of PLK1.
- MALDI-IMS combined with shotgun proteomics identify and localize new factors in male infertility
In situ proteomics of male infertility.
- Reduced RNA turnover as a driver of cellular senescence
RNAs originating from transcription upstream and downstream of genes accumulate in the cytoplasm of a subset of senescent cells, suggesting an RNA alternative to cytoplasmic DNA in the triggering of senescence.
- ER targeting of non-imported mitochondrial carrier proteins is dependent on the GET pathway
The GET pathway is required to target non-imported mitochondrial carrier proteins to the endoplasmic reticulum, which prevents their deposition into Hsp42-dependent protein foci.
- Insulin signaling mediates neurodegeneration in glioma
Tumoral cells secrete an antagonist that attenuates insulin signaling in neurons. It induces mitochondrial defects and synapse loss; restoring neuronal insulin activity rescues neurodegeneration.
Methods
- A FIJI macro for quantifying pattern in extracellular matrix
This work provides a quantitative tool for the analysis of fibrillar patterns in biology. We demonstrates its utility on in vitro generated extracellular matrices, mouse tissues, and patient samples. The tool is already publicly available to the research community.