ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes

Arnaud Rondelet; Andrei Pozniakovsky; Devika Namboodiri; Richard Cardoso da Silva; Divya Singh; Marit Leuschner; Ina Poser; Andrea Ssykor; Julian Berlitz; Nadine Schmidt; Lea Röhder; Gerben Vader; Anthony A Hyman; Alexander W Bird

doi:10.26508/lsa.202000836

Research Article

Open Access

ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes

Arnaud Rondelet, Andrei Pozniakovsky, View ORCID ProfileDevika Namboodiri, Richard Cardoso da Silva, Divya Singh, Marit Leuschner, Ina Poser, Andrea Ssykor, Julian Berlitz, Nadine Schmidt, Lea Röhder, View ORCID ProfileGerben Vader, Anthony A Hyman, View ORCID ProfileAlexander W Bird Correspondence email

Arnaud Rondelet

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Andrei Pozniakovsky

2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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Devika Namboodiri

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Richard Cardoso da Silva

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Divya Singh

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Marit Leuschner

2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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Ina Poser

2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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Andrea Ssykor

2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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Julian Berlitz

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Nadine Schmidt

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Lea Röhder

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Gerben Vader

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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Anthony A Hyman

2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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Alexander W Bird

1Max Planck Institute of Molecular Physiology, Dortmund, Germany

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For correspondence: alex.bird@mpi-dortmund.mpg.de

Published 8 December 2020. DOI: 10.26508/lsa.202000836

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ESI mutagenesis of bacterial artificial chromosomes

Arnaud Rondelet, Andrei Pozniakovsky, Devika Namboodiri, Richard Cardoso da Silva, Divya Singh, Marit Leuschner, Ina Poser, Andrea Ssykor, Julian Berlitz, Nadine Schmidt, Lea Röhder, Gerben Vader, Anthony A Hyman, Alexander W Bird

Life Science Alliance Dec 2020, 4 (2) e202000836; DOI: 10.26508/lsa.202000836