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Research Articles
Expression of immunoglobulin constant domain genes in neurons of the mouse central nervous systemIn this study, Scheurer et al demonstrate that two genes encoding the effector domain of immunoglobulins are not only expressed by lymphocytes of the immune system but also by neurons in the central nervous system.
Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2The context-dependency of genetic interactions of the cohesin gene STAG2, specifically STAG1 and the iron regulatory gene IREB2, are revealed by CRISPR-Cas9 screening in three different cellular backgrounds.
Statistical guidelines for quality control of next-generation sequencing techniquesCondition-specific statistical guidelines and accurate classification trees for quality control of functional genomics NGS files (RNA-seq, ChIP-seq and DNase-seq) have been generated using thousands of reference files from the ENCODE project and made available to the community.
High levels of TFAM repress mammalian mitochondrial DNA transcription in vivoThe TFAM-to-mtDNA ratio is critically important and must be maintained within a physiological range to allow normal mtDNA expression and prevent nucleoid hypercompaction in different mouse tissues.
Biallelic ADPRHL2 mutations in complex neuropathy affect ADP ribosylation and DNA damage responseThis work studies known and new ADPRHL2 mutations with different disease mechanisms overall indicating that loss of nuclear ARH3 alone is pathogenic via dysregulated nuclear ADP ribosylation.
Ccq1–Raf2 interaction mediates CLRC recruitment to establish heterochromatin at telomeresThis study unveils a role of Ccq1–Raf2 interaction in CLRC recruitment to establish heterochromatin at telomeres, providing a positive feedback mechanism between shelterin and CLRC for subtelomeric heterochromatin assembly.
Autoimmune anti-DNA and anti-phosphatidylserine antibodies predict development of severe COVID-19COVID-19 induces high levels of autoimmune anti-DNA and anti-phosphatidylserine antibodies that are detected in some patients upon hospital admission and predict later development of severe disease.
Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signalingThis study analyses the function of the protein tyrosine phosphatase 1B identifying its binding partners and dephosphorylation targets for modulating B cell antigen receptor signaling.
The Th1 cell regulatory circuitry is largely conserved between human and mouseComparison of the binding sites of the Th1 lineage-specifying transcription factor T-bet between human and mouse reveals differences in binding sites but that the most of the target genes are shared.
An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasitesMalaria arises during the proliferation of Plasmodium spp. in human blood, whereas the underlying atypical cell division mechanisms remain poorly understood. This study uses advanced imaging to dissect dynamics and organization of the centrosome, a key division regulator.
Choroid plexus APP regulates adult brain proliferation and animal behaviorAdult mouse choroid plexus shows elevated APP expression. sAPPα secreted into the CSF modulates neurogenic niche proliferation, whereas choroid plexus expression of fAD APP mutants leads to reduced niche proliferation, deficits in hippocampus synaptic plasticity, and learning defects.
Elevated glycolysis imparts functional ability to CD8+ T cells in HIV infectionCytotoxic killer CD8+ T cells (CTLs) are central effectors that eliminate virus infected cells. In HIV infection, HIV-specific CTLs become functionally impaired. Herein we investigated the link between metabolism and CD8+ T-cell functional impairment, known as exhaustion.
Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in miceOptogenetic activation of FGFR2 allowed temporally precise induction of signaling and behavioural changes, but counter-regulation at multiple levels prevented a sustained response in keratinocytes.
INPP5K and Atlastin-1 maintain the nonuniform distribution of ER–plasma membrane contacts in neuronsCIL-1 and ATLN-1 maintain the balance between ER tubules and sheets and prevent invasion of cortical ER sheets into the axon, contributing to the non-uniform distribution of neuronal ER-PM contacts.
SATB1-dependent mitochondrial ROS production controls TCR signaling in CD4 T cellsSATB1 regulates mitochondrial function and reactive oxygen species (ROS) production through the expression of mitochondrial transcription factor A. SATB1-mediated ROS production is necessary for TCR stimulation and T-cell function.
