Table of Contents
Research Articles
Incomplete antiviral treatment may induce longer durations of viral shedding during SARS-CoV-2 infectionTreatments initiated early, such as 0.5 d after virus inoculation, with intermediate to relatively high efficacy (30–70% inhibition of virus replication) yield a prolonged duration of viral shedding (by about 6.0 d) compared with no treatment.
Integrated human/SARS-CoV-2 metabolic models present novel treatment strategies against COVID-19Integrated human/SARS-CoV-2 metabolic models present novel treatment strategies against COVID-19 and provide insights into viral entry inhibition, immune regulation, and drug optimisation strategies.
Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cellsTissue-resident memory (TRM) T cells in mouse and human melanoma-associated vitiligo skin form large lymphoid aggregates with CXCL16-expressing dendritic cells. CD11c depletion or disruption of the CXCR6-CXCL16 axis results in loss of skin TRM cells and tumor immunity.
Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoidsHere, the authors evaluate prime editing in human adult stem cell–derived organoids. TP53 mutations are modeled in hepatocyte and colon organoids and the clinical application of prime editing is evaluated by repairing mutations in CFTR without genome-wide off-targets.
Extracellular matrix mediates moruloid-blastuloid morphodynamics in malignant ovarian spheroidsExpression dynamics of basement membrane-like extracellular matrix and fibronectin regulate transitions between solid (moruloid) and cavitational (blastuloid) phenotypes of ovarian cancer spheroids.
Tyrosine phosphorylation of lamin A by Src promotes disassembly of nuclear lamina in interphasePhosphorylation of lamin A at tyrosine residue 45 by Src has an adverse effect on the assembly of nuclear lamina, which may be involved in nuclear dysmorphia, genomic instability, and nuclear plasticity in interphase.
Dystrophin involvement in peripheral circadian SRF signallingAbsence of integral sarcolemmal protein, dystrophin, disrupts the RhoA-actin-SRF cascade in skeletal muscle, with subsequent dysregulation of downstream-SRF circadian targets and circadian rhythm.
AMOTL2 mono-ubiquitination by WWP1 promotes contact inhibition by facilitating LATS activationThis work reveals a novel function of WWP1 E3 ligase in the mono-ubiquitination of AMOTL2, which enables the binding and activation of LATS kinases upon contact inhibition.
RNA sequencing reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubesRNA sequencing reveals primary myocyte-specific gene-regulatory niche effects for β-hydroxybutyrate but do not support a general signaling of β-hydroxybutyrate role in other primary cells or during cellular differentiation in vitro.
Improved systemic AAV gene therapy with a neurotrophic capsid in Niemann–Pick disease type C1 miceThis work highlights the importance of CNS transduction for treatment of neurological diseases, a finding with significant clinical implications considering the long-lasting effects of gene therapy.
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sitesPlant SYT1 transports lipids between endoplasmic reticulum and plasma membrane in response to stress. The protein flexibility and lipid-binding specificity determine SYT1 function at molecular level.
A role for Rad5 in ribonucleoside monophosphate (rNMP) toleranceRibonucleoside incorporation in genomic DNA poses a significant threat to genomic integrity. Here, we describe how cells tolerate this threat and discuss implications for cancer therapeutics.
Elevated glucose increases genomic instability by inhibiting nucleotide excision repairExposure to chronic, elevated glucose inhibits nucleotide excision repair, which leads to accumulation of DNA glycation adducts, increased DNA strand breaks, and activation of the DNA damage response.
Serum proteomics reveals disorder of lipoprotein metabolism in sepsisThis study illustrated that lipoprotein and lipid metabolism might play a significant role in patients with sepsis and that complement activation was significantly enriched in patients with sepsis-associated encephalopathy.
Glucocorticoid signaling induces transcriptional memory and universally reversible chromatin changesGlucocorticoids are stress hormones that elicit various cellular responses. These responses are typically reversible; however, in some instances, a previous exposure is “remembered” and influences the response to a subsequent hormone encounter.
