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TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells

Dorothea Bestle, Miriam Ruth Heindl, Hannah Limburg, View ORCID ProfileThuy Van Lam van, Oliver Pilgram, Hong Moulton, View ORCID ProfileDavid A Stein, Kornelia Hardes, Markus Eickmann, View ORCID ProfileOlga Dolnik, Cornelius Rohde, Hans-Dieter Klenk, Wolfgang Garten, Torsten Steinmetzer, View ORCID ProfileEva Böttcher-Friebertshäuser  Correspondence email
Dorothea Bestle
1Institute of Virology, Philipps-University, Marburg, Germany
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Miriam Ruth Heindl
1Institute of Virology, Philipps-University, Marburg, Germany
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Hannah Limburg
1Institute of Virology, Philipps-University, Marburg, Germany
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Thuy Van Lam van
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
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  • ORCID record for Thuy Van Lam van
Oliver Pilgram
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
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Hong Moulton
3Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
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David A Stein
3Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
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  • ORCID record for David A Stein
Kornelia Hardes
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
4Fraunhofer Institute for Molecular Biology and Applied Ecology, Gießen, Germany
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Markus Eickmann
1Institute of Virology, Philipps-University, Marburg, Germany
5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
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Olga Dolnik
1Institute of Virology, Philipps-University, Marburg, Germany
5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
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  • ORCID record for Olga Dolnik
Cornelius Rohde
1Institute of Virology, Philipps-University, Marburg, Germany
5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
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Hans-Dieter Klenk
1Institute of Virology, Philipps-University, Marburg, Germany
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Wolfgang Garten
1Institute of Virology, Philipps-University, Marburg, Germany
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Torsten Steinmetzer
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
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Eva Böttcher-Friebertshäuser
1Institute of Virology, Philipps-University, Marburg, Germany
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  • ORCID record for Eva Böttcher-Friebertshäuser
  • For correspondence: friebertshaeuser@staff.uni-marburg.de
Published 23 July 2020. DOI: 10.26508/lsa.202000786
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TMPRSS2 and furin activate SARS-CoV-2
Dorothea Bestle, Miriam Ruth Heindl, Hannah Limburg, Thuy Van Lam van, Oliver Pilgram, Hong Moulton, David A Stein, Kornelia Hardes, Markus Eickmann, Olga Dolnik, Cornelius Rohde, Hans-Dieter Klenk, Wolfgang Garten, Torsten Steinmetzer, Eva Böttcher-Friebertshäuser
Life Science Alliance Jul 2020, 3 (9) e202000786; DOI: 10.26508/lsa.202000786

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TMPRSS2 and furin activate SARS-CoV-2
Dorothea Bestle, Miriam Ruth Heindl, Hannah Limburg, Thuy Van Lam van, Oliver Pilgram, Hong Moulton, David A Stein, Kornelia Hardes, Markus Eickmann, Olga Dolnik, Cornelius Rohde, Hans-Dieter Klenk, Wolfgang Garten, Torsten Steinmetzer, Eva Böttcher-Friebertshäuser
Life Science Alliance Jul 2020, 3 (9) e202000786; DOI: 10.26508/lsa.202000786
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Volume 3, No. 9
September 2020
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  • FXa cleaves the SARS-CoV-2 spike protein and blocks cell entry to protect against infection with inferior effects in B.1.1.7 variant
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  • Term Human Placental Trophoblasts Express SARS-CoV-2 Entry Factors ACE2, TMPRSS2, and Furin
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  • The SARS-CoV-2 and other human coronavirus spike proteins are fine-tuned towards temperature and proteases of the human airways
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