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TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells

Dorothea Bestle, Miriam Ruth Heindl, Hannah Limburg, View ORCID ProfileThuy Van Lam van, Oliver Pilgram, Hong Moulton, View ORCID ProfileDavid A Stein, Kornelia Hardes, Markus Eickmann, View ORCID ProfileOlga Dolnik, Cornelius Rohde, Hans-Dieter Klenk, Wolfgang Garten, Torsten Steinmetzer, View ORCID ProfileEva Böttcher-Friebertshäuser  Correspondence email
Dorothea Bestle
1Institute of Virology, Philipps-University, Marburg, Germany
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Miriam Ruth Heindl
1Institute of Virology, Philipps-University, Marburg, Germany
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Hannah Limburg
1Institute of Virology, Philipps-University, Marburg, Germany
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Thuy Van Lam van
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
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  • ORCID record for Thuy Van Lam van
Oliver Pilgram
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
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Hong Moulton
3Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
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David A Stein
3Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
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  • ORCID record for David A Stein
Kornelia Hardes
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
4Fraunhofer Institute for Molecular Biology and Applied Ecology, Gießen, Germany
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Markus Eickmann
1Institute of Virology, Philipps-University, Marburg, Germany
5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
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Olga Dolnik
1Institute of Virology, Philipps-University, Marburg, Germany
5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
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Cornelius Rohde
1Institute of Virology, Philipps-University, Marburg, Germany
5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
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Hans-Dieter Klenk
1Institute of Virology, Philipps-University, Marburg, Germany
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Wolfgang Garten
1Institute of Virology, Philipps-University, Marburg, Germany
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Torsten Steinmetzer
2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
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Eva Böttcher-Friebertshäuser
1Institute of Virology, Philipps-University, Marburg, Germany
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  • ORCID record for Eva Böttcher-Friebertshäuser
  • For correspondence: friebertshaeuser@staff.uni-marburg.de
Published 23 July 2020. DOI: 10.26508/lsa.202000786
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Article Information

vol. 3 no. 9 e202000786
DOI 
https://doi.org/10.26508/lsa.202000786
PubMed 
32703818

Published By 
Life Science Alliance
Online ISSN 
2575-1077
History 
  • Received May 20, 2020
  • Revision received July 15, 2020
  • Accepted July 15, 2020
  • Published online July 23, 2020.

Copyright & Usage 
© 2020 Bestle et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

Author Information

  1. Dorothea Bestle1,*,
  2. Miriam Ruth Heindl1,*,
  3. Hannah Limburg1,*,
  4. Thuy Van Lam van2,
  5. Oliver Pilgram2,
  6. Hong Moulton3,
  7. David A Stein3,
  8. Kornelia Hardes2,4,
  9. Markus Eickmann1,5,
  10. Olga Dolnik1,5,
  11. Cornelius Rohde1,5,
  12. Hans-Dieter Klenk1,
  13. Wolfgang Garten1,
  14. Torsten Steinmetzer2 and
  15. Eva Böttcher-Friebertshäuser1⇑
  1. 1Institute of Virology, Philipps-University, Marburg, Germany
  2. 2Institute of Pharmaceutical Chemistry, Philipps-University, Marburg, Germany
  3. 3Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
  4. 4Fraunhofer Institute for Molecular Biology and Applied Ecology, Gießen, Germany
  5. 5German Center for Infection Research (DZIF), Marburg-Gießen-Langen Site, Emerging Infections Unit, Philipps-University, Marburg, Germany
  1. Correspondence: friebertshaeuser{at}staff.uni-marburg.de
  1. ↵* Dorothea Bestle, Miriam Ruth Heindl, and Hannah Limburg contributed equally to this work

View Full Text

Funding

  • LOEWE Center DRUID

    D1
  • Deutsche Forschungsgemeinschaft (DFG)

    SFB 1021, B07
  • German Center for Infection Research (DZIF)

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TMPRSS2 and furin activate SARS-CoV-2
Dorothea Bestle, Miriam Ruth Heindl, Hannah Limburg, Thuy Van Lam van, Oliver Pilgram, Hong Moulton, David A Stein, Kornelia Hardes, Markus Eickmann, Olga Dolnik, Cornelius Rohde, Hans-Dieter Klenk, Wolfgang Garten, Torsten Steinmetzer, Eva Böttcher-Friebertshäuser
Life Science Alliance Jul 2020, 3 (9) e202000786; DOI: 10.26508/lsa.202000786

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TMPRSS2 and furin activate SARS-CoV-2
Dorothea Bestle, Miriam Ruth Heindl, Hannah Limburg, Thuy Van Lam van, Oliver Pilgram, Hong Moulton, David A Stein, Kornelia Hardes, Markus Eickmann, Olga Dolnik, Cornelius Rohde, Hans-Dieter Klenk, Wolfgang Garten, Torsten Steinmetzer, Eva Böttcher-Friebertshäuser
Life Science Alliance Jul 2020, 3 (9) e202000786; DOI: 10.26508/lsa.202000786
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Volume 3, No. 9
September 2020
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