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Fungal mycelia and bacterial thiamine establish a mutualistic growth mechanism

View ORCID ProfileGayan Abeysinghe, Momoka Kuchira, Gamon Kudo, View ORCID ProfileShunsuke Masuo, Akihiro Ninomiya, Kohei Takahashi, View ORCID ProfileAndrew S Utada, Daisuke Hagiwara, Nobuhiko Nomura, Naoki Takaya, View ORCID ProfileNozomu Obana  Correspondence email, View ORCID ProfileNorio Takeshita  Correspondence email
Gayan Abeysinghe
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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  • ORCID record for Gayan Abeysinghe
Momoka Kuchira
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Gamon Kudo
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Shunsuke Masuo
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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  • ORCID record for Shunsuke Masuo
Akihiro Ninomiya
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Kohei Takahashi
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Andrew S Utada
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Daisuke Hagiwara
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Nobuhiko Nomura
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Naoki Takaya
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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Nozomu Obana
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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  • ORCID record for Nozomu Obana
  • For correspondence: obana.nozomu.gb@u.tsukuba.ac.jp
Norio Takeshita
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
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  • For correspondence: takeshita.norio.gf@u.tsukuba.ac.jp
Published 22 September 2020. DOI: 10.26508/lsa.202000878
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Abstract

Exclusivity in physical spaces and nutrients is a prerequisite for survival of organisms, but a few species have been able to develop mutually beneficial strategies that allow them to co-habit. Here, we discovered a mutualistic mechanism between filamentous fungus, Aspergillus nidulans, and bacterium, Bacillus subtilis. The bacterial cells co-cultured with the fungus traveled along mycelia using their flagella and dispersed farther with the expansion of fungal colony, indicating that the fungal mycelia supply space for bacteria to migrate, disperse, and proliferate. Transcriptomic, genetic, molecular mass, and imaging analyses demonstrated that the bacteria reached the mycelial edge and supplied thiamine to the growing hyphae, which led to a promotion of hyphal growth. The thiamine transfer from bacteria to the thiamine non-auxotrophic fungus was directly demonstrated by stable isotope labeling. The simultaneous spatial and metabolic interactions demonstrated in this study reveal a mutualism that facilitates the communicating fungal and bacterial species to obtain an environmental niche and nutrient, respectively.

  • Received August 16, 2020.
  • Revision received September 7, 2020.
  • Accepted September 14, 2020.
  • © 2020 Abeysinghe et al.
Creative Commons logoCreative Commons logohttps://creativecommons.org/licenses/by/4.0/

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Fungal highway and bacterial toll for their mutualism
Gayan Abeysinghe, Momoka Kuchira, Gamon Kudo, Shunsuke Masuo, Akihiro Ninomiya, Kohei Takahashi, Andrew S Utada, Daisuke Hagiwara, Nobuhiko Nomura, Naoki Takaya, Nozomu Obana, Norio Takeshita
Life Science Alliance Sep 2020, 3 (12) e202000878; DOI: 10.26508/lsa.202000878

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Fungal highway and bacterial toll for their mutualism
Gayan Abeysinghe, Momoka Kuchira, Gamon Kudo, Shunsuke Masuo, Akihiro Ninomiya, Kohei Takahashi, Andrew S Utada, Daisuke Hagiwara, Nobuhiko Nomura, Naoki Takaya, Nozomu Obana, Norio Takeshita
Life Science Alliance Sep 2020, 3 (12) e202000878; DOI: 10.26508/lsa.202000878
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Volume 3, No. 12
December 2020
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