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Table of Contents

November 2020 | Volume 3, No. 11

Research Articles

  • PGE<sub>2</sub> accounts for bidirectional changes in alveolar macrophage self-renewal with aging and smoking
    Open Access
    PGE2 accounts for bidirectional changes in alveolar macrophage self-renewal with aging and smoking

    Loka R Penke, Jennifer M Speth, Christina Draijer, Zbigniew Zaslona, Judy Chen, Peter Mancuso, Christine M Freeman, Jeffrey L Curtis, Daniel R Goldstein, Marc Peters-Golden

    Loka R Penke ... Marc Peters-Golden

    Published 20 August 2020

    Dysregulation of self-renewal contributes to alterations in alveolar macrophages (AMs). In this study, Penke et al identify PGE2 as a suppressor of AM proliferation, whose divergent bioavailability in aged and cigarette smoke-exposed mice could explain opposite effects on AM numbers.

  • Plasma membrane phosphatidylinositol (4,5)-bisphosphate promotes Weibel–Palade body exocytosis
    Open Access
    Plasma membrane phosphatidylinositol (4,5)-bisphosphate promotes Weibel–Palade body exocytosis

    Tu Thi Ngoc Nguyen, Sophia N Koerdt, Volker Gerke

    Tu Thi Ngoc Nguyen ... Volker Gerke

    Published 21 August 2020

    Phosphatidylinositol (4,5)-bisphosphate transiently accumulates at sites of Weibel–Palade body–plasma membrane fusion and promotes agonist-evoked exocytosis of endothelial von-Willebrand factor.

  • Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assembly
    Open Access
    Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assembly

    María Ascensión Villar-Fernández, Richard Cardoso da Silva, Magdalena Firlej, Dongqing Pan, Elisabeth Weir, Annika Sarembe, Vivek B Raina, Tanja Bange, John R Weir, Gerben Vader

    María Ascensión Villar-Fernández ... Gerben Vader

    Published 21 August 2020

    The AAA+ protein Pch2 forms a biochemical complex with Orc1/ORC to suppress DNA break formation in the meiotic G2/prophase.

  • An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR
    Open Access
    An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR

    Shih-Hsing Leir, Shiyi Yin, Jenny L Kerschner, Wilmel Cosme, Ann Harris

    Shih-Hsing Leir ... Ann Harris

    Published 27 August 2020

    A coordinated process of maturation in the lumen of the epididymis is required for sperm to fertilize an egg. A single cell atlas of the human proximal epididymis reveals key cell-specific functions, regional specialization, and two roles for CFTR.

  • Multiplexed chemogenetics in astrocytes and motoneurons restore blood–spinal cord barrier in ALS
    Open Access
    Multiplexed chemogenetics in astrocytes and motoneurons restore blood–spinal cord barrier in ALS

    Najwa Ouali Alami, Linyun Tang, Diana Wiesner, Barbara Commisso, David Bayer, Jochen Weishaupt, Luc Dupuis, Phillip Wong, Bernd Baumann, Thomas Wirth, Tobias M Boeckers, Deniz Yilmazer-Hanke, Albert Ludolph, Francesco Roselli

    Najwa Ouali Alami ... Francesco Roselli

    Published 8 September 2020

    Chemogenetic motoneuron excitation and astrocyte GPCR-Gi signaling restore blood–spinal cord barrier, disrupted in four ALS mouse models, revealing its role in disease progression but not initiation.

  • multicrispr: gRNA design for prime editing and parallel targeting of thousands of targets
    Open Access
    multicrispr: gRNA design for prime editing and parallel targeting of thousands of targets

    Aditya M Bhagwat, Johannes Graumann, Rene Wiegandt, Mette Bentsen, Jordan Welker, Carsten Kuenne, Jens Preussner, Thomas Braun, Mario Looso

    Aditya M Bhagwat ... Mario Looso

    Published 9 September 2020

    A bioinformatics R package for a fast and generic guideRNA design, optimized for large-scale perturbation assays.

  • MicroRNA regulation of murine trophoblast stem cell self-renewal and differentiation
    Open Access
    MicroRNA regulation of murine trophoblast stem cell self-renewal and differentiation

    Sarbani Saha, Rupasri Ain

    Sarbani Saha ... Rupasri Ain

    Published 9 September 2020

    Post-transcriptional regulation of CDX2 and cell cycle genes by miR-290 and miR-322 clusters along with transactivation of miR-290 cluster and cyclin D1 by CDX2 equipoise trophoblast stem cell self-renewal and differentiation.

  • Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4
    Open Access
    Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4

    Noushin Akhavantabib, Daniel D Krzizike, Victoria Neumann, Sheena D’Arcy

    Noushin Akhavantabib ... Sheena D’Arcy

    Published 10 September 2020

    The work determines the relative and absolute stoichiometry of a 5-chain protein complex involved in histone chaperoning and acetylation. Using sedimentation velocity and light scattering, it uncovers a dynamic equilibrium of complex self-association.

  • Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F
    Open Access
    Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F

    Wout Oosterheert, Katerina T Xenaki, Viviana Neviani, Wouter Pos, Sofia Doulkeridou, Jip Manshande, Nicholas M Pearce, Loes MJ Kroon-Batenburg, Martin Lutz, Paul MP van Bergen en Henegouwen, Piet Gros

    Wout Oosterheert ... Piet Gros

    Published 21 September 2020

    Crystal and single-particle cryo-EM structures reveal how the tetraspanin CD9 interacts with its prototypical partner EWI-F and provide a new concatenation model for the assembly of tetraspanin-enriched microdomains.

  • Human G-MDSCs are neutrophils at distinct maturation stages promoting tumor growth in breast cancer
    Open Access
    Human G-MDSCs are neutrophils at distinct maturation stages promoting tumor growth in breast cancer

    Meliha Mehmeti-Ajradini, Caroline Bergenfelz, Anna-Maria Larsson, Robert Carlsson, Kristian Riesbeck, Jonas Ahl, Helena Janols, Marlene Wullt, Anders Bredberg, Eva Källberg, Frida Björk Gunnarsdottir, Camilla Rydberg Millrud, Lisa Rydén, Gesine Paul, Niklas Loman, Jörgen Adolfsson, Ana Carneiro, Karin Jirström, Fredrika Killander, Daniel Bexell, Karin Leandersson

    Meliha Mehmeti-Ajradini ... Karin Leandersson

    Published 21 September 2020

    This study shows that immunosuppressive primary breast cancer patient–derived G-MDSCs (PMN-MDSCs) are neutrophils at a range of maturations stages, and provides in vivo evidence for that human G-MDSCs also promote tumor growth and myeloid immune cell exclusion.

  • Predicting gene regulatory networks from cell atlases
    Open Access
    Predicting gene regulatory networks from cell atlases

    Andreas Fønss Møller, Kedar Nath Natarajan

    Andreas Fønss Møller ... Kedar Nath Natarajan

    Published 21 September 2020

    Integrated single-cell gene regulatory network from three mouse cell atlases captures global and cell type–specific regulatory modules and crosstalk, important for cellular identity.

  • Gene selection for optimal prediction of cell position in tissues from single-cell transcriptomics data
    Open Access
    Gene selection for optimal prediction of cell position in tissues from single-cell transcriptomics data

    Jovan Tanevski, Thin Nguyen, Buu Truong, Nikos Karaiskos, Mehmet Eren Ahsen, Xinyu Zhang, Chang Shu, Ke Xu, Xiaoyu Liang, Ying Hu, Hoang VV Pham, Li Xiaomei, Thuc D Le, Adi L Tarca, Gaurav Bhatti, Roberto Romero, Nestoras Karathanasis, Phillipe Loher, Yang Chen, Zhengqing Ouyang, Disheng Mao, Yuping Zhang, Maryam Zand, Jianhua Ruan, Christoph Hafemeister, Peng Qiu, Duc Tran, Tin Nguyen, Attila Gabor, Thomas Yu, Justin Guinney, Enrico Glaab, Roland Krause, Peter Banda, DREAM SCTC Consortium, Gustavo Stolovitzky, Nikolaus Rajewsky, Julio Saez-Rodriguez, Pablo Meyer

    Jovan Tanevski ... Pablo Meyer

    Published 24 September 2020

    We describe and provide an array of diverse methods to predict cellular positions in tissue from RNA-seq data selecting mapping genes according to their spatial/statistical properties and their effect on improving the cell positioning.

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Volume 3, No. 11
November 2020
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