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Research Articles
- PGE2 accounts for bidirectional changes in alveolar macrophage self-renewal with aging and smoking
Dysregulation of self-renewal contributes to alterations in alveolar macrophages (AMs). In this study, Penke et al identify PGE2 as a suppressor of AM proliferation, whose divergent bioavailability in aged and cigarette smoke-exposed mice could explain opposite effects on AM numbers.
- Plasma membrane phosphatidylinositol (4,5)-bisphosphate promotes Weibel–Palade body exocytosis
Phosphatidylinositol (4,5)-bisphosphate transiently accumulates at sites of Weibel–Palade body–plasma membrane fusion and promotes agonist-evoked exocytosis of endothelial von-Willebrand factor.
- Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assembly
The AAA+ protein Pch2 forms a biochemical complex with Orc1/ORC to suppress DNA break formation in the meiotic G2/prophase.
- An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTR
A coordinated process of maturation in the lumen of the epididymis is required for sperm to fertilize an egg. A single cell atlas of the human proximal epididymis reveals key cell-specific functions, regional specialization, and two roles for CFTR.
- Multiplexed chemogenetics in astrocytes and motoneurons restore blood–spinal cord barrier in ALS
Chemogenetic motoneuron excitation and astrocyte GPCR-Gi signaling restore blood–spinal cord barrier, disrupted in four ALS mouse models, revealing its role in disease progression but not initiation.
- multicrispr: gRNA design for prime editing and parallel targeting of thousands of targets
A bioinformatics R package for a fast and generic guideRNA design, optimized for large-scale perturbation assays.
- MicroRNA regulation of murine trophoblast stem cell self-renewal and differentiation
Post-transcriptional regulation of CDX2 and cell cycle genes by miR-290 and miR-322 clusters along with transactivation of miR-290 cluster and cyclin D1 by CDX2 equipoise trophoblast stem cell self-renewal and differentiation.
- Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4
The work determines the relative and absolute stoichiometry of a 5-chain protein complex involved in histone chaperoning and acetylation. Using sedimentation velocity and light scattering, it uncovers a dynamic equilibrium of complex self-association.
- Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F
Crystal and single-particle cryo-EM structures reveal how the tetraspanin CD9 interacts with its prototypical partner EWI-F and provide a new concatenation model for the assembly of tetraspanin-enriched microdomains.
- Human G-MDSCs are neutrophils at distinct maturation stages promoting tumor growth in breast cancer
This study shows that immunosuppressive primary breast cancer patient–derived G-MDSCs (PMN-MDSCs) are neutrophils at a range of maturations stages, and provides in vivo evidence for that human G-MDSCs also promote tumor growth and myeloid immune cell exclusion.
- Predicting gene regulatory networks from cell atlases
Integrated single-cell gene regulatory network from three mouse cell atlases captures global and cell type–specific regulatory modules and crosstalk, important for cellular identity.
- Gene selection for optimal prediction of cell position in tissues from single-cell transcriptomics data
We describe and provide an array of diverse methods to predict cellular positions in tissue from RNA-seq data selecting mapping genes according to their spatial/statistical properties and their effect on improving the cell positioning.