Table of Contents
Research Articles
PGE2 accounts for bidirectional changes in alveolar macrophage self-renewal with aging and smokingDysregulation of self-renewal contributes to alterations in alveolar macrophages (AMs). In this study, Penke et al identify PGE2 as a suppressor of AM proliferation, whose divergent bioavailability in aged and cigarette smoke-exposed mice could explain opposite effects on AM numbers.
Plasma membrane phosphatidylinositol (4,5)-bisphosphate promotes Weibel–Palade body exocytosisPhosphatidylinositol (4,5)-bisphosphate transiently accumulates at sites of Weibel–Palade body–plasma membrane fusion and promotes agonist-evoked exocytosis of endothelial von-Willebrand factor.
Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assemblyThe AAA+ protein Pch2 forms a biochemical complex with Orc1/ORC to suppress DNA break formation in the meiotic G2/prophase.
An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTRA coordinated process of maturation in the lumen of the epididymis is required for sperm to fertilize an egg. A single cell atlas of the human proximal epididymis reveals key cell-specific functions, regional specialization, and two roles for CFTR.
Multiplexed chemogenetics in astrocytes and motoneurons restore blood–spinal cord barrier in ALSChemogenetic motoneuron excitation and astrocyte GPCR-Gi signaling restore blood–spinal cord barrier, disrupted in four ALS mouse models, revealing its role in disease progression but not initiation.
multicrispr: gRNA design for prime editing and parallel targeting of thousands of targetsA bioinformatics R package for a fast and generic guideRNA design, optimized for large-scale perturbation assays.
MicroRNA regulation of murine trophoblast stem cell self-renewal and differentiationPost-transcriptional regulation of CDX2 and cell cycle genes by miR-290 and miR-322 clusters along with transactivation of miR-290 cluster and cyclin D1 by CDX2 equipoise trophoblast stem cell self-renewal and differentiation.
Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4The work determines the relative and absolute stoichiometry of a 5-chain protein complex involved in histone chaperoning and acetylation. Using sedimentation velocity and light scattering, it uncovers a dynamic equilibrium of complex self-association.
Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-FCrystal and single-particle cryo-EM structures reveal how the tetraspanin CD9 interacts with its prototypical partner EWI-F and provide a new concatenation model for the assembly of tetraspanin-enriched microdomains.
Human G-MDSCs are neutrophils at distinct maturation stages promoting tumor growth in breast cancerThis study shows that immunosuppressive primary breast cancer patient–derived G-MDSCs (PMN-MDSCs) are neutrophils at a range of maturations stages, and provides in vivo evidence for that human G-MDSCs also promote tumor growth and myeloid immune cell exclusion.
Predicting gene regulatory networks from cell atlasesIntegrated single-cell gene regulatory network from three mouse cell atlases captures global and cell type–specific regulatory modules and crosstalk, important for cellular identity.
