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MERLIN: a novel BRET-based proximity biosensor for studying mitochondria–ER contact sites

View ORCID ProfileVanessa Hertlein, View ORCID ProfileHector Flores-Romero, Kushal K Das, Sebastian Fischer, Michael Heunemann, View ORCID ProfileMaria Calleja-Felipe, Shira Knafo, View ORCID ProfileKatharina Hipp, Klaus Harter, Julia C Fitzgerald, View ORCID ProfileAna J García-Sáez  Correspondence email
Vanessa Hertlein
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany
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  • ORCID record for Vanessa Hertlein
Hector Flores-Romero
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany
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  • ORCID record for Hector Flores-Romero
Kushal K Das
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany
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Sebastian Fischer
2University of Heidelberg, Heidelberg, Germany
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Michael Heunemann
3Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany
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Maria Calleja-Felipe
4Molecular Cognition Laboratory, Biophysics Institute, Consejo Superior de Investigaciones Cientificas, University of the Basque Country (UPV)/Euskal Herriko University, Campus Universidad del País Vasco, Leioa, Spain
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Shira Knafo
4Molecular Cognition Laboratory, Biophysics Institute, Consejo Superior de Investigaciones Cientificas, University of the Basque Country (UPV)/Euskal Herriko University, Campus Universidad del País Vasco, Leioa, Spain
5Ikerbasque, Basque Foundation for Science, Bilbao, Spain
6Department of Physiology and Cell Biology and National Institute of Biotechnology in the Negev, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
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Katharina Hipp
7Max Planck Institute for Developmental Biology, Tübingen, Germany
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Klaus Harter
3Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany
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Julia C Fitzgerald
8Hertie-Institute for Clinical Brain Research, University of Tübingen and German Centre for Neurodegenerative Diseases (DZNE), Tübingen, Germany
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Ana J García-Sáez
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany
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  • ORCID record for Ana J García-Sáez
  • For correspondence: ana.garcia@uni-koeln.de
Published 9 December 2019. DOI: 10.26508/lsa.201900600
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Article Information

vol. 3 no. 1 e201900600
DOI 
https://doi.org/10.26508/lsa.201900600
PubMed 
31818884

Published By 
Life Science Alliance
Online ISSN 
2575-1077
History 
  • Received November 12, 2019
  • Revision received November 25, 2019
  • Accepted November 27, 2019
  • Published online December 9, 2019.

Copyright & Usage 
© 2019 Hertlein et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

Author Information

  1. Vanessa Hertlein1,*,
  2. Hector Flores-Romero1,*,
  3. Kushal K Das1,
  4. Sebastian Fischer2,
  5. Michael Heunemann3,
  6. Maria Calleja-Felipe4,
  7. Shira Knafo4,5,6,
  8. Katharina Hipp7,
  9. Klaus Harter3,
  10. Julia C Fitzgerald8 and
  11. Ana J García-Sáez1⇑
  1. 1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany
  2. 2University of Heidelberg, Heidelberg, Germany
  3. 3Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany
  4. 4Molecular Cognition Laboratory, Biophysics Institute, Consejo Superior de Investigaciones Cientificas, University of the Basque Country (UPV)/Euskal Herriko University, Campus Universidad del País Vasco, Leioa, Spain
  5. 5Ikerbasque, Basque Foundation for Science, Bilbao, Spain
  6. 6Department of Physiology and Cell Biology and National Institute of Biotechnology in the Negev, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
  7. 7Max Planck Institute for Developmental Biology, Tübingen, Germany
  8. 8Hertie-Institute for Clinical Brain Research, University of Tübingen and German Centre for Neurodegenerative Diseases (DZNE), Tübingen, Germany
  1. Correspondence: ana.garcia{at}uni-koeln.de
  1. ↵* Vanessa Hertlein and Hector Flores-Romero contributed equally to this work

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MERLIN, mitochondria–ER length indicator nanosensor
Vanessa Hertlein, Hector Flores-Romero, Kushal K Das, Sebastian Fischer, Michael Heunemann, Maria Calleja-Felipe, Shira Knafo, Katharina Hipp, Klaus Harter, Julia C Fitzgerald, Ana J García-Sáez
Life Science Alliance Dec 2019, 3 (1) e201900600; DOI: 10.26508/lsa.201900600

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MERLIN, mitochondria–ER length indicator nanosensor
Vanessa Hertlein, Hector Flores-Romero, Kushal K Das, Sebastian Fischer, Michael Heunemann, Maria Calleja-Felipe, Shira Knafo, Katharina Hipp, Klaus Harter, Julia C Fitzgerald, Ana J García-Sáez
Life Science Alliance Dec 2019, 3 (1) e201900600; DOI: 10.26508/lsa.201900600
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Volume 3, No. 1
January 2020
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