Table of Contents
Research Articles
Targeting plasma membrane phosphatidylserine content to inhibit oncogenic KRAS functionKRAS-dependent cancer cell growth is inhibited by disrupting phosphatidylserine transport to the plasma membrane by genetic knockdown of lipid exchangers ORP5 and ORP8 or by inhibition of PI4KIIIα.
Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in miceMutation of three cationic amino acids previously identified as an extracellular phosphatidylserine-binding motif abrogates ADAM17 sheddase function and causes early embryonic death in mice.
Endogenous RNAi pathway evolutionarily shapes the destiny of the antisense lncRNAs transcriptomeA genome-wide comparative analysis of “cryptic” aslncRNAs decay in RNAi-capable and RNAi-deficient budding yeasts suggests an evolutionary contribution of RNAi in shaping the aslncRNAs transcriptome.
Structural and functional characterization of the mitochondrial complex IV assembly factor Coa6The structures of the mitochondrial complex IV assembly factor Coa6 and a pathogenic mutant variant (W59CCoa6) are reported, providing molecular mechanisms for its mode of action and the loss-of-function mutation.
The huntingtin inclusion is a dynamic phase-separated compartmentWhen expressed in yeast, mutant Htt-GFP forms an inclusion that is mobile, gel-like, and releases material; evidence suggests that it grows through collision and coalescence with small aggregates.
Pleiotropic requirements for human TDP-43 in the regulation of cell and organelle homeostasisComprehensive analysis of human TDP-43 knockout cells revealed that TDP-43 regulates hundreds of transcripts and is critical for maintaining the homeostasis of multiple subcellular organelles.
Mechanistic insights into the protective roles of polyphosphate against amyloid cytotoxicityThis study provides novel insights into the mechanisms by which presence of polyP alters the formation, structural properties, and cytotoxic effects of α-synuclein fibers.
Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachineThe article shows successful engineering of the syringe-like injection apparatus of Photorhabdus luminescens as standalone nanomachine for delivery of protein toxins and enzymes from different species into mammalian cells.
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modificationsThe glycine cleavage system (GCS) is highly activated to promote stem cell pluripotency. The GCS catabolizes glycine to prevent methylglyoxal accumulation and to fuel H3K4me3 modification, promoting the expression of pluripotency genes.
Sufficiency analysis of estrogen responsive enhancers using synthetic activatorsEnhancers bound by synthetic activators can recreate a transcriptional response to estrogen, drive different levels of gene expression, and work independently to regulate transcription.
HELZ directly interacts with CCR4–NOT and causes decay of bound mRNAsThe putative UPF1-like SF1 helicase HELZ directly interacts with the CCR4–NOT deadenylase complex to induce translational repression and 5′-to-3′ decay of bound mRNAs.
Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forksFft3, a member of the SNF2 family of ATPase-dependent enzymes, plays a dual role at DNA replication barriers in fission yeast by promoting both DNA resection and restart of blocked DNA replication forks.
Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency markerExpression of TET proteins in pluripotent cell types is visualised by epitope tagging of endogenous alleles.
Ligand-induced IFNGR1 down-regulation calibrates myeloid cell IFNγ responsivenessIFNγ is shown to silence Ifngr1 transcription by altering enhancer occupancy, reducing myeloid cell IFNGR1 as a feedback mechanism to attenuate macrophage responses associated with inflammation.
Enhanced carbonyl stress induces irreversible multimerization of CRMP2 in schizophrenia pathogenesisEnhanced carbonyl stress results in neurodevelopmental deficits by affecting microtubule function through the formation of irreversible dysfunctional multimer of carbonylated CRMP2.
Oct4 mediates Müller glia reprogramming and cell cycle exit during retina regeneration in zebrafishThe rapid induction of pluripotency-inducing factor Oct4 in the injured retina necessitates the de novo induction of stem cells and their subsequent cell cycle exit.
Resolving kinesin stepping: one head at a timeThis study shows for the first time a qualitative difference in the stepping of a heterodimeric motor and the asymmetric influence of autoinhibition on the stepping of one of the heads.
Unfolded protein-independent IRE1 activation contributes to multifaceted developmental processes in ArabidopsisThe Arabidopsis unfolded protein response transducer IRE1 contributes to male gametophyte development using an alternative activation mechanism bypassing the unfolded protein-sensing domain.
Drug-induced chromatin accessibility changes associate with sensitivity to liver tumor promotionThis work explores quantitative chromatin accessibility, transcriptional and cis-acting gene regulatory variations underlying mouse strain–specific differences in drug-induced liver tumor promotion sensitivity.
Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and diseaseOxidative protein folding in the ER generates ROS, leading to Nrf2-dependent feedback on protein folding via ER calcium level modulation. This feedback loop is suppressed in ALS-associated mutant astrocytes but can be rescued by dimethyl fumarate.
Methods
Detecting sequence signals in targeting peptides using deep learningDuring the development of TargetP 2.0, a state-of-the-art method to predict targeting signal, we find a previously overlooked biological signal for subcellular targeting using the output from a deep learning method.
Resource
Defining the expression of piRNA and transposable elements in Drosophila ovarian germline stem cells and somatic support cellsComprehensive transcriptional characterization of mRNA and small RNA in early Drosophila germline stem cells reveals novel piRNA clusters, transposon dynamics, and alternative splicing events.
Conservation of cell-intrinsic immune responses in diverse nonhuman primate speciesThe transcriptomic response of diverse nonhuman primate (NHP) species to poly(I:C) is highly conserved, and this novel RNA sequencing dataset will help improve NHP genome annotations.
