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Gene symbol Gene name Forward sequence (5′-3′) Reverse sequence (5′-3′) Human primers ATG4D Autophagy related 4D cysteine peptidase CTCAACCCCGTGTATGTGC TACAGTGAGTGTCGCGGTTT ATG9B Autophagy related 9B GCTACTGGGACATCCAGGTG AAGAGGCGGGACTGCAC CAT Catalase ACTTTGAGGTCACACATGACATT CTGAACCCGATTCTCCAGCA FIS1 Mitochondrial fission 1 protein TGACATCCGTAAAGGCATCG CTTCTCGTATTCCTTGAGCCG GPX Glutathione peroxidase 1 CCAGTCGGTGTATGCCTTCTC GAGGGACGCCACATTCTCG HMOX1 Heme oxygenase 1 GCCAGCAACAAAGTGCAAG GAGTGTAAGGACCCATCGGA HPRT1 Hypoxanthine phosphoribosyltransferase 1 CCTGGCGTCGTGATTAGTGAT AGACGTTCAGTCCTGTCCATAA LAMP1 Lysosomal-associated membrane protein 1 ACGTTACAGCGTCCAGCTCAT TCTTTGGAGCTCGCATTGG MAP1LC3B Microtubule associated protein 1 light chain 3 beta GAGAAGACCTTCAAGCAGCG TATCACCGGGATTTTGGTTG MCOLN1 Mucolipin 1 TTGCTCTCTGCCAGCGGTACTA GCAGTCAGTAACCACCATCGGA MFN2 Mitofusin 2 ATGTGGCCCAACTCTAAGTG CACAAACACATCAGCATCCAG PPARGC1A Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha AGCCTCTTTGCCCAGATCTT CTGATTGGTCACTGCACCAC PPARGC1B Peroxisome proliferator-activated receptor gamma, coactivator 1 beta CCACATCCTACCCAACATCAAG CACAAGGCCGTTGACTTTTAGA POLG DNA polymerase gamma, catalytic subunit GAAGGACATTCGTGAGAACTTCC GTGGGGACACCTCTCCAAG PPIA Peptidylprolyl isomerase A CAGACAAGGTCCCAAAGACAG TTGCCATCCAACCACTCAGTC PRC PPARG related coactivator 1 (PPRC1) GTGGTTGGGGAAGTCGAAG TGACAAAGCCAGAATCACCC SOD1 Superoxide dismutase 1, soluble AGGGCATCATCAATTTCGAGC GCCCACCGTGTTTTCTGGA SOD2 Superoxide dismutase 2, mitochondrial CAGACCTGCCTTACGACTATGG CGTTCAGGTTGTTCACGTAGG SIRT1 Sirtuin 1 TCAGTGTCATGGTTCCTTTGC AATCTGCTCCTTTGCCACTCT SIRT3 Sirtuin 3 TGGAAAGCCTAGTGGAGCTTCTGGG TGGGGGCAGCCATCATCCTATTTGT TFAM Transcription factor a, mitochondrial CCATATTTAAAGCTCAGAACCCAG CTCCGCCCTATAAGCATCTTG TP53 Tumor protein p53 TCAACAAGATGTTTTGCCAACTG ATGTGCTGTGACTGCTTGTAGATG TXN2 Thioredoxin 2 TGATGACCACACAGACCTCG ATCCTTGATGCCCACAAACT TWIST1 Twist family BHLH transcription factor 1 CGGAGAAGCTGAGCAAGATT TGGAGGACCTGGTAGAGGAA VIM Vimentin GAGAACTTTGCCGTTGAAGC TCCAGCAGCTTCCTGTAGGT-3 WIPI1 WD repeat domain, phosphoinositide interacting 1 CCTCCTGGATATTCCTGCAA GCACAATCTCCCCTGAAGTC ZEB1 Zinc finger E-box binding homeobox 1 AGTGATCCAGCCAAATGGAA TTTTTGGGCGGTGTAGAATC ZEB2 Zinc finger E-box binding homeobox 2 AACAAGCCAATCCCAGGAG GTTGGCAATACCGTCATCCT Mouse primers Hprt1 Hypoxanthine phosphoribosyltransferase 1 TCAGTCAACGGGGGACATAAA GGGGCTGTACTGCTTAACCAG Ppargc1a Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha AGCCGTGACCACTGACAACGAG GTCGCATGGTTCTGAGTGCTAAG Ppargc1b Peroxisome proliferator-activated receptor gamma, coactivator 1 beta CCCAGCGTCTGACGTGGACGAGC CCTTCAGAGCGTCAGAGCTTGCTG Ppia Peptidylprolyl isomerase A GAGCTGTTTGCAGACAAAGTTC CCCTGGCACATGAATCCTGG
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