Table of Contents
Research Articles
Substrate fluxes in brown adipocytes upon adrenergic stimulation and uncoupling protein 1 ablationQuantification of substrate fluxes in brown adipocytes clarifies contributors to extracellular acidification, identifies a futile cycle of lipolysis and re-esterification, and suggests a role for glycogen during activation.
Plk1 regulates spindle orientation by phosphorylating NuMA in human cellsThis work reveals that Plk1 phosphorylates an evolutionarily conserved protein NuMA in human cells and this phosphorylation negatively regulates NuMA and thus dynein cortical localization in metaphase that is crucial for proper spindle orientation.
The BMP2/4 ortholog Dpp can function as an inter-organ signal that regulates developmental timingIncreased local trapping of morphogens within tissues as they grow would reduce circulating levels and can therefore provide a systemic readout of the status of their growth and maturation.
Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DCMature dendritic cells (DC) secrete substantial amounts of vesicles that are primarily ingested by monocytes, leading to differentiation processes in these target cells towards monocyte-derived DC.
Peroxisome biogenesis deficiency attenuates the BDNF-TrkB pathway-mediated development of the cerebellumPeroxisome biogenesis deficiency leads to increased expression of BDNF and of a truncated form of the BDNF receptor in the cerebellum, attenuates BDNF-TrkB signaling, and results in malformation of the cerebellum.
Dimerization and auto-processing induce caspase-11 protease activation within the non-canonical inflammasomeThis study provides a detailed molecular mechanism for caspase-11 activation within the non-canonical inflammasome, giving new insight into host defence against cytosolic bacterial infection.
Mouse REC114 is essential for meiotic DNA double-strand break formation and forms a complex with MEI4Mouse REC114 is essential for meiotic DNA double-strand break formation and forms a complex with IHO1. Its N-terminal region forms a Pleckstrin homology domain, while its C-terminal region is interacting with MEI4.
A role for MED14 and UVH6 in heterochromatin transcription upon destabilization of silencingThe TFIIH component UVH6 and the mediator subunit MED14 are differentially required for the release of heterochromatin silencing, and MED14 regulates non-CG DNA methylation in Arabidopsis.
Variants of DNMT3A cause transcript-specific DNA methylation patterns and affect hematopoiesisModulation of DNMT3A splice variants causes transcript-specific DNA methylation and gene expression changes and affects differentiation. Particularly, transcript 2 is relevant in acute myeloid leukemia.
Absence of MHC-II expression by lymph node stromal cells results in autoimmunityMHCII-restricted antigen presentation by lymph node stromal cells is essential for regulatory T-cell proliferation and functions, and for the regulation of autoimmunity.
Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPaseNovel GAK phosphorylation targets are identified using chemical genetic methods. One of the substrates is the α subunit of the Na+/K+-ATPase, phosphorylation of which is necessary for its surface trafficking from endosomes. Conserved functions of NAK family kinases are described.
Direct binding of Cdt2 to PCNA is important for targeting the CRL4Cdt2 E3 ligase activity to Cdt1The C-terminal end of Cdt2 contains a PIP box for binding to PCNA to promote CRL4Cdt2 function, creating a new paradigm where the substrate receptor and substrates bind to a common multivalent docking platform for ubiquitination.
Methods
An integrative machine learning approach for prediction of toxicity-related drug safetyThis method allows the prediction of toxicity-related drug clinical trial failures, withdrawals from the market, and idiosyncratic toxicity risk by combining biological network analysis with machine learning.
Resources
A carnivorous plant genetic map: pitcher/insect-capture QTL on a genetic linkage map of SarraceniaThis study presents the first genetic map for a carnivorous plant, mapping 64 QTLs in Sarracenia to provide the genetic basis for differences between the pitfall and lobster-trap insect-capture strategies.
Re-annotation of eight Drosophila genomesUsing a well-annotated genome to inform the annotation of genomes in a phylogeny.
