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Research Articles
Dynamic reorganisation of intermediate filaments coordinates early B-cell activationThis study examines the role of vimentin, a type III intermediate filament, in B-cell function using a combination of in vitro and in vivo assays, including super-resolution microscopic techniques.
Mitochondrial ubiquinone–mediated longevity is marked by reduced cytoplasmic mRNA translationThis study uses polysomal RNA sequencing to show that the translational efficiency of specific mRNA changes in long-lived Caenorhabditis elegans with reduced ubiquinone synthesis.
MBNL1 alternative splicing isoforms play opposing roles in cancerMBNL1 proteins lacking exon 7 (−ex7) are antisurvival factors with tumor suppressive role that cancer cells tend to down-regulate in favor of MBNL +ex7 isoforms.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethalityMouse embryos with an ablated ability of integrins to bind laminins are still able to form basement membranes, but die just after implantation because of deficient extraembryonic development.
Transposon silencing in the Drosophila female germline is essential for genome stability in progeny embryosSuppression of transposons by the Piwi-interacting RNA biogenesis factor Vasa in the supporting nurse cells is essential to prevent their accumulation in the developing oocyte, ensuring proper Drosophila embryonic development.
Identification of Plasmodium GAPDH epitopes for generation of antibodies that inhibit malaria infectionThis study reports on the identification of two Plasmodium GAPDH epitope peptides that are responsible for sporozoite–Kupffer cell interaction and act as antigens against malaria infection.
CARM1 methylates MED12 to regulate its RNA-binding abilityCARM1 methylates MED12 at arginine 1899 to generate a TDRD3 binding site, which in turn regulates the ability of mediator to interact with activating ncRNAs and modulate gene expression.
S-phase transcriptional buffering quantified on two different promotersTranscriptional buffering enforced during DNA replication shows that histone acetylation governs the homeostasis process and can also restrict promoters from reaching maximum transcriptional potential
Interaction modulation through arrays of clustered methyl-arginine protein modificationsExtensively modifiable arrays of clustered arginines in a large set of human proteins function as regulatory protein interaction platforms. Quantitative immunoprecipitation assays defined two distinct cumulative binding mechanisms operating across an array of 18 methyl-arginine motifs in SYNCRIP.
Loss of HDAC11 ameliorates clinical symptoms in a multiple sclerosis mouse modelIn an animal model for multiple sclerosis, the absence of HDAC11 reduces clinical severity, spinal cord demyelination, and immune cell infiltration, suggesting that HDAC11 is a promising target for MS treatment.
Secretory granule protein chromogranin B (CHGB) forms an anion channel in membranesThe CHGB subfamily of secretory granule proteins forms a new family of anion-selective channels by interacting with membranes via two amphipathic α-helices. The channel exhibits higher anion selectivity, larger conductance, higher DIDS-binding affinity, and higher Cl− sensitivity than other known anion channels.
Specificity for deubiquitination of monoubiquitinated FANCD2 is driven by the N-terminus of USP1Deubiquitination of FANCD2, FANCI, and PCNA by USP1 is essential for DNA repair signalling. Reconstitution of the system reveals that USP1-mediated specificity towards K561 of FANCD2 is directed by a unique sequence at USP1's N-terminus.
The crystal structure of Staufen1 in complex with a physiological RNA sheds light on substrate selectivityCombination of in vitro and in vivo data show that RNA sequence influences Staufen target recognition and that protein–RNA base contacts are required for Staufen function in Drosophila.
Topological in vitro loading of the budding yeast cohesin ring onto DNAThe biochemical reconstitution of topological DNA binding by budding yeast cohesin yields surprises and opens opportunities to exploit experimental approaches developed in this model organism.
Disruption of stromal hedgehog signaling initiates RNF5-mediated proteasomal degradation of PTEN and accelerates pancreatic tumor growthDisrupting paracrine Hedgehog signaling in pancreatic cancer stroma through genetic deletion of fibroblast Smoothened leads to proteasomal degradation of fibroblast PTEN and accelerates tumor growth.
Phosphorylation of Arp2 is not essential for Arp2/3 complex activity in fission yeastThis work employed genomic substitutions blocking or mimicking phosphorylation at three proposed phosphorylation sites on Arp2 to demonstrate that they do not regulate Arp2/3 complex activity in fission yeast.
LATS1 and LATS2 suppress breast cancer progression by maintaining cell identity and metabolic stateIn luminal B tumors LATS2 depletion results in metabolic rewiring whereas LATS1 depletion promotes the expression of basal-like features.
Methods
Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA librariesHigh-throughput inverse toeprinting identifies peptide-encoding transcripts that induce ribosome stalling and allows the systematic analysis of sequence-dependent translational events.
Resource
Small RNA-Seq reveals novel miRNAs shaping the transcriptomic identity of rat brain structuresSmall RNA-Seq of the rat central nervous system reveals known and novel miRNAs specifically regulated in brain structures and correlated with the expression of their predicted target genes, suggesting a critical role in the transcriptomic identity of brain structures.
