Chromatin & Epigenetics
Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assemblyHigh content image analysis, single molecule tracking, modeling, and DBA patient analysis revealed that ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assembly.
DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteinsLong-term epigenetic gene silencing can be induced independently of DNA methylation by dCas9-KRABd-MeCP2 fusion proteins.
AGO1 regulates pericentromeric regions in mouse embryonic stem cellsDepletion of AGO1 in mESCs leads to a redistribution of H3K9me3 and HP1α away from pericentromeric regions and is accompanied by an up-regulation of major satellites transcripts.
Angioplasty induces epigenomic remodeling in injured arteriesThis is the first in vivo epigenomic survey revealing genome-wide loci-specific chromatin mark redistribution after angioplasty; the underlying epigenetic regulations may inform therapeutic targeting.
Refining the domain architecture model of the replication origin firing factor Treslin/TICRRThe replication origin firing factor Treslin/TICRR comprises an essential Sld3-like core that requires the flanking Ku70-like N-terminal and the C-terminal domains for efficient origin firing.
Cis-regulatory hubs: a new 3D model of complex disease genetics with an application to schizophreniaGenes and their regulatory elements are organized in neurons within 3D networks which model functional structures and explain schizophrenia genetic etiology.
Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resectionDSB recruitment of the cohesin loader Scc2/4 relies on Tel1 and γH2A, but contrary to cohesin not on Mec1; binding emanates from the break site and depends on as well as coincides with end resection.
Epigenetic modifier balances Mapk and Wnt signalling in differentiation of goblet and Paneth cellsThe histone methyltransferase Mll1 controls intestinal secretory cell fate by promoting Wnt-driven Paneth and restricting Mapk-dependent goblet cell differentiation through regulation of Gata4/6 transcription factors
Retinoblastoma protein regulates carcinogen susceptibility at heterochromatic cancer driver lociThe retinoblastoma protein regulates mutagenic potential via control of UV-induced DNA lesion acquisition, thus revealing novel mechanisms that contribute to its tumor suppressor capabilities.
Generation of human long-lived plasma cells by developmentally regulated epigenetic imprintingThis study shows that the generation of human long-lived plasma cells requires blood antibody secreting cells to undergo epigenetic and transcriptional reprogramming in response to the bone marrow microniche to become apoptosis resistant and survive to secrete antibodies for a lifetime.