Chromatin & Epigenetics
RSC and GRFs confer promoter directionality by restricting divergent noncoding transcriptionWe show that the chromatin remodelling complex RSC and general regulatory factors (GRFs) limit transcription in the noncoding divergent direction, thereby increasing the directionality of promoters.
Mesenchymal stem cells derived from patients with premature aging syndromes display hallmarks of physiological agingUsing of genome-wide approaches on models from laminopathies with or without progerin accumulation (HGPS, HGPS-L, APS), this study provides new insights on pathways altered during early stages of mesenchymal stem cells differentiation.
Sibling rivalry among the ZBTB transcription factor family: homodimers versus heterodimersBTB domains potentially can form homo- or heterodimers. The study examines the dimerization choice of several BTB domains and finds only one heterodimer, while all tested pairs can homodimerize.
Emetine blocks DNA replication via proteosynthesis inhibition not by targeting Okazaki fragmentsThis study clarifies the long-standing misuse of emetine as a lagging strand synthesis-specific inhibitor. Emetine blocks the synthesis of both DNA strands by the inhibition of protein biosynthesis.
Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changesThe meiotic DNA break response is shown to involve a large number of phosphorylation changes and a clearly detectable transcriptional response. By contrast, the meiotic proteome remains remarkably unchanged in response to breaks.
Mitotic H3K9ac is controlled by phase-specific activity of HDAC2, HDAC3, and SIRT1Combination of immunofluorescence, Western blot, and ChIP-seq revealed the interplay between HDAC2, HDAC3, and SIRT1 in H3K9 deacetylation during mitosis of mammalian cells.
The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delaysChromosome structuring and condensation is one of the main features of mitosis. Here, Matos-Perdomo et al show how the nuclear envelope reshapes around the vacuole to give rise to the outstanding ribosomal DNA loop in budding yeast.
Drosophila insulator proteins exhibit in vivo liquid–liquid phase separation propertiesDrosophila insulator proteins and the cohesin subunit Rad21 coalesce in vivo to form liquid-droplet condensates, suggesting that liquid–liquid phase separation mediates their function in 3D genome organization.
The MLL3/4 complexes and MiDAC co-regulate H4K20ac to control a specific gene expression programThe MLL3/4 histone methyltransferase complexes and the histone deacetylase complex MiDAC regulate the histone mark H4K20ac to calibrate gene expression of a common set of target genes.
chromMAGMA: regulatory element-centric interrogation of risk variantschromMAGMA, a pipeline that prioritizes candidate risk regulatory elements and target genes, reveals novel risk-associated genes when applied to epithelial ovarian cancer.