Chromatin & Epigenetics
- De novo discovery of traits co-occurring with chronic obstructive pulmonary disease
This paper integrates different levels of biological information to identify shared genetic and biological pathways underlying the diverse clinical presentations of COPD. Thus, it identifies a collection of genetic variants and genes that may aid COPD patient stratification and treatment.
- ZBTB18 inhibits SREBP-dependent lipid synthesis by halting CTBPs and LSD1 activity in glioblastoma
This study identifies a new mechanism of fatty acid synthesis regulation in glioblastoma that involves ZBTB18, CTBP, and LSD1. The transcriptional repressor ZBTB18 interacts with CTBP and inhibits LSD1 demethylase activity.
- Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN
We present a detailed step-wise protocol for reliable genome-wide profiling of histone modifications using CUT&RUN that requires only low numbers of Plasmodium falciparum blood stages.
- A critique of the hypothesis that CA repeats are primary targets of neuronal MeCP2
Using relevant neuronal datasets and in vitro experiments, we show that MeCP2 function depends on methylated CAC (mCAC) sites regardless of their occurrence in CA repeats or elsewhere in the genome.
- RSC and GRFs confer promoter directionality by restricting divergent noncoding transcription
We show that the chromatin remodelling complex RSC and general regulatory factors (GRFs) limit transcription in the noncoding divergent direction, thereby increasing the directionality of promoters.
- Mesenchymal stem cells derived from patients with premature aging syndromes display hallmarks of physiological aging
Using of genome-wide approaches on models from laminopathies with or without progerin accumulation (HGPS, HGPS-L, APS), this study provides new insights on pathways altered during early stages of mesenchymal stem cells differentiation.
- Sibling rivalry among the ZBTB transcription factor family: homodimers versus heterodimers
BTB domains potentially can form homo- or heterodimers. The study examines the dimerization choice of several BTB domains and finds only one heterodimer, while all tested pairs can homodimerize.
- Emetine blocks DNA replication via proteosynthesis inhibition not by targeting Okazaki fragments
This study clarifies the long-standing misuse of emetine as a lagging strand synthesis-specific inhibitor. Emetine blocks the synthesis of both DNA strands by the inhibition of protein biosynthesis.
- Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changes
The meiotic DNA break response is shown to involve a large number of phosphorylation changes and a clearly detectable transcriptional response. By contrast, the meiotic proteome remains remarkably unchanged in response to breaks.
- Mitotic H3K9ac is controlled by phase-specific activity of HDAC2, HDAC3, and SIRT1
Combination of immunofluorescence, Western blot, and ChIP-seq revealed the interplay between HDAC2, HDAC3, and SIRT1 in H3K9 deacetylation during mitosis of mammalian cells.