Chromatin & Epigenetics
RB loss sensitizes cells to replication-associated DNA damage after PARP inhibition by trappingAfter PARP inhibition by trapping, cells lacking the RB tumor suppressor experience persistent replication stress and genomic instability that compromise cell viability.
Chromatin in 3D distinguishes dMes-4/NSD and Hypb/dSet2 in protecting genes from H3K27me3 silencingHistone KMTs of H3K36me maintains euchromatin by antagonizing H3K27me3 spreading and influencing 3D genome organization at TADs boundaries, thus participating in transcriptional program maintenance.
Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryosThis study shows that failed activation of developmentally required Alyref and Gabpb1 genes leads to the arrest of cloned mouse embryos.
c-JUN is a barrier in hESC to cardiomyocyte transitionThe study reveals that knocking out c-JUN improves cardiomyocyte generation in humans by increasing chromatin accessibility and H3K4me3 modification on key genes regulating heart development. These findings shed new light on c-JUN's unexpected role in human cardiogenesis.
Dispersal of PRC1 condensates disrupts polycomb chromatin domains and loopsEndogenous PRC1-mediated chromatin compaction and clustering of polycomb target loci is reversibly perturbed in mESCs by the addition of 1,6 hexanediol, which can disrupt biomolecular condensates.
A SAM-key domain required for enzymatic activity of the Fun30 nucleosome remodelerBiochemical data suggest that the SAM-key domain of the budding yeast remodeler Fun30 is required for its nucleosome remodeling activity by regulating the catalytic ATPase.
Lsd1 safeguards T-cell development via suppressing endogenous retroelements and interferon responsesBulk and single-cell RNA-seq, combined with ChIP-seq analysis, reveal that the loss of Lsd1 leads to the aberrant depression of endogenous retroelements and subsequent IFN response signaling in thymocytes, which are possible causes of impaired early T cell development.
Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20This work characterizes the transcriptional and epigenetic control of memory CD8 T cell differentiation by the BTB-ZF family transcription factor Zbtb20 using single cell RNA and ATAC sequencing and identifies direct genomic targets of Zbtb20 using CUT&RUN.
The NSL complex is required for piRNA production from telomeric clustersGermline knockdown of the NSL complex leads to transposon derepression, particularly of telomeric transposons. NSL2 binds promoters of telomeric transposons and its depletion leads to alterations in the chromatin state of multiple telomeric piRNA clusters.
Hypoxia controls expression of kidney-pathogenic MUC1 variantsHypoxia or pharmacological treatment with novel HIF stabilizers promotes the expression of MUC1 genetic variants that predispose to the development of chronic kidney disease in renal tubular cells.