Chromatin & Epigenetics
Lsd1 safeguards T-cell development via suppressing endogenous retroelements and interferon responsesBulk and single-cell RNA-seq, combined with ChIP-seq analysis, reveal that the loss of Lsd1 leads to the aberrant depression of endogenous retroelements and subsequent IFN response signaling in thymocytes, which are possible causes of impaired early T cell development.
Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20This work characterizes the transcriptional and epigenetic control of memory CD8 T cell differentiation by the BTB-ZF family transcription factor Zbtb20 using single cell RNA and ATAC sequencing and identifies direct genomic targets of Zbtb20 using CUT&RUN.
The NSL complex is required for piRNA production from telomeric clustersGermline knockdown of the NSL complex leads to transposon derepression, particularly of telomeric transposons. NSL2 binds promoters of telomeric transposons and its depletion leads to alterations in the chromatin state of multiple telomeric piRNA clusters.
Hypoxia controls expression of kidney-pathogenic MUC1 variantsHypoxia or pharmacological treatment with novel HIF stabilizers promotes the expression of MUC1 genetic variants that predispose to the development of chronic kidney disease in renal tubular cells.
Inter and transgenerational impact of H3K4 methylation in neuronal homeostasisThe study highlights the importance of maintaining appropriate H3K4me3 levels to prevent negative impacts on nerve system homeostasis across generations.
Methylation of lysine 36 on histone H3 is required to control transposon activities in somatic cellsDisruption of H3K36 methylation in somatic cells leads to transposon transcription and activation of key genes in the PIWI-interacting RNA pathway together with RNA production from piRNA clusters.
Cohesin SMC1β promotes closed chromatin and controls TERRA expression at spermatocyte telomeresCohesin SMC1β; ensures silencing at chromosome ends, restricts TERRA expression and RNA-DNA hybrid formation, and prevents telomer damage.
BiFCo: visualizing cohesin assembly/disassembly cycle in living cellsA novel strategy developed in fission yeast to visualise only-assembled cohesin complex loading/unloading cycles in eukaryotic chromosomes in living cells.
BAZ2A-RNA mediated association with TOP2A and KDM1A represses genes implicated in prostate cancerBAZ2A-mediated repression in prostate cancer is regulated by the RNA-binding TAM domain. The BAZ2A-TAM domain, in association with RNA, promotes the interaction with TOP2A and KDM1A that serves to repress genes critical to prostate cancer progression.
Dynamic changes of enhancer and super enhancer landscape in degenerated nucleus pulposus cellsSuper-enhancers mediate inflammatory cascade and extracellular matrix remodeling in degenerated nucleus pulposus cells.