Chromatin & Epigenetics
- Intrinsic deletion at 10q23.31, including the PTEN gene locus, is aggravated upon CRISPR-Cas9–mediated genome engineering in HAP1 cells mimicking cancer profiles
The unintended 10q23.31 deletion frequency rises in HAP1 cells stressed during CRISPR-Cas9 engineering, leading to aberrant molecular and cellular changes resembling common cancer patient deletions.
- Transgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposure
We exposed pregnant female mice to neonicotinoid thiacloprid. We observed reproductive defects together with DNA methylation and histone H3K9me3 changes in the third-generation males.
- Evolutionary insights into 3D genome organization and epigenetic landscape of Vigna mungo
By integrating SMRT long read, Hi-C, and Illumina pair-end sequencing technologies, we report a nearly complete, chromosomal-level assembly of V. mungo and characterize its 3D genome architecture.
- Polycomb regulates circadian rhythms in Drosophila in clock neurons
This study demonstrated that Pc regulates the circadian rhythm. The targeted DamID analysis identified the Pc-binding profiles in different clock neuron clusters, indicating Pc regulates circadian rhythms by specific clock genes.
- Correction: Dispersal of PRC1 condensates disrupts polycomb chromatin domains and loops
Endogenous PRC1–mediated chromatin compaction and clustering of polycomb target loci are reversibly perturbed in mESCs by the addition of 1,6 hexandiol.
- Cost-effective DNA methylation profiling by FML-seq
A low-cost, high-throughput method for genome-wide DNA methylation profiling based on methylation-specific restriction digestion and sequencing.
- RB loss sensitizes cells to replication-associated DNA damage after PARP inhibition by trapping
After PARP inhibition by trapping, cells lacking the RB tumor suppressor experience persistent replication stress and genomic instability that compromise cell viability.
- Chromatin in 3D distinguishes dMes-4/NSD and Hypb/dSet2 in protecting genes from H3K27me3 silencing
Histone KMTs of H3K36me maintains euchromatin by antagonizing H3K27me3 spreading and influencing 3D genome organization at TADs boundaries, thus participating in transcriptional program maintenance.
- Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos
This study shows that failed activation of developmentally required Alyref and Gabpb1 genes leads to the arrest of cloned mouse embryos.
- c-JUN is a barrier in hESC to cardiomyocyte transition
The study reveals that knocking out c-JUN improves cardiomyocyte generation in humans by increasing chromatin accessibility and H3K4me3 modification on key genes regulating heart development. These findings shed new light on c-JUN's unexpected role in human cardiogenesis.