Chromatin & Epigenetics
- Epigenomic states contribute to coordinated allelic transcriptional bursting in iPSC reprogramming
Epigenomic states are linked to the coordinated allelic transcriptional bursting of genes involved in iPSC reprogramming.
- Mapping parental DMRs predictive of local and distal methylome remodeling in epigenetic F1 hybrids
We show that parental DNA methylation divergence in pericentromeric regions predicts non-additivity in the methylomes, transcriptomes, and phenotypes of F1 hybrids, independently of DNA sequence divergence.
- Spatiotemporal DNA methylation dynamics shape megabase-scale methylome landscapes
The guanine–cytosine content of genomic regions influences their spatial properties, resulting in various megabase-scale methylome patterns across different cell types.
- Chromatin targeting of the RNF12/RLIM E3 ubiquitin ligase controls transcriptional responses
The E3 ubiquitin ligase RNF12/RLIM is mutated in intellectual disability and controls developmental gene expression. We show that chromatin targeting of RNF12 is required for substrate ubiquitylation and transcriptional responses, providing insight into mechanisms by which ubiquitylation regulates gene expression.
- Changes in searching behaviour of CSL transcription complexes in Notch active conditions
Single molecule live imaging of the Notch pathway transcription factor CSL reveals how its properties change in Notch active nuclei, acquiring exploratory behaviour.
- 2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibition
This study investigates the genome-wide effects of 2-HG accumulation on chromatin and gene expression and identifies Rph1-dependent and H3K36me3-associated gene repression at silenced loci in yeast.
- Chromatin priming elements direct tissue-specific gene activity before hematopoietic specification
This study shows that signalling processes prime many enhancer elements at the chromatin level before the expression of their associated genes and that priming is essential for developmental stage-specific dynamic gene activation.
- Intrinsic deletion at 10q23.31, including the PTEN gene locus, is aggravated upon CRISPR-Cas9–mediated genome engineering in HAP1 cells mimicking cancer profiles
The unintended 10q23.31 deletion frequency rises in HAP1 cells stressed during CRISPR-Cas9 engineering, leading to aberrant molecular and cellular changes resembling common cancer patient deletions.
- Transgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposure
We exposed pregnant female mice to neonicotinoid thiacloprid. We observed reproductive defects together with DNA methylation and histone H3K9me3 changes in the third-generation males.
- Evolutionary insights into 3D genome organization and epigenetic landscape of Vigna mungo
By integrating SMRT long read, Hi-C, and Illumina pair-end sequencing technologies, we report a nearly complete, chromosomal-level assembly of V. mungo and characterize its 3D genome architecture.