Chromatin & Epigenetics
Subunit interactions and arrangements in the fission yeast Mis16–Mis18–Mis19 complexA structural study of the fission yeast Mis19 protein, required for centromere establishment, is presented together with data describing its interactions with functional partners Mis16 and Mis18.
Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutantsThis study characterizes the sequence, end structure, and length distribution of Chlamydomonas reinhardtii telomeres and shows that telomerase mutants are defective in telomere maintenance.
Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activityAnalysis of the mitotic replisome disassembly pathway in X. laevis egg extract shows that any replisomes retained on chromatin past S-phase are unloaded through formation of K6- and K63-linked ubiquitin chains on Mcm7 by TRAIP ubiquitin ligase and p97/VCP activity.
Intra-individual methylomics detects the impact of early-life adversityThis study shows that methylation profile changes across time in the same individual distinguish a stressful experience from typical infancy, providing a potential predictive marker of vulnerability to disease.
BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBVHow herpesviruses escape from their latent states is enigmatic, but BZLF1, an important transcription factor of EBV, recruits cellular chromatin remodelers to viral DNA to overcome its epigenetic repression and initiate viral transcription.
Assessment and site-specific manipulation of DNA (hydroxy-)methylation during mouse corticogenesisThis work describes the dynamics of DNA modifications in specific cell types of the developing mammalian cortex. By providing a new method to manipulate this process in vivo, it is shown how this process can influence brain formation.
Mitochondrial acetyl-CoA reversibly regulates locus-specific histone acetylation and gene expressionThis study shows that genetic or pharmacological manipulation of the TCA cycle, when mitochondria are dysfunctional, can modulate histone acetylation and gene expression in the nucleus with physiological outcomes.
Polycomb and Notch signaling regulate cell proliferation potential during Caenorhabditis elegans life cycleCaenorhabditis elegans lineage is invariant between animals. By challenging cell fate in differentiated worms, an unexpected role of Polycomb and Notch signaling in the control of cell proliferation was uncovered, suggesting that the lineage is more flexible than believed.
Variants of DNMT3A cause transcript-specific DNA methylation patterns and affect hematopoiesisModulation of DNMT3A splice variants causes transcript-specific DNA methylation and gene expression changes and affects differentiation. Particularly, transcript 2 is relevant in acute myeloid leukemia.
A role for MED14 and UVH6 in heterochromatin transcription upon destabilization of silencingThe TFIIH component UVH6 and the mediator subunit MED14 are differentially required for the release of heterochromatin silencing, and MED14 regulates non-CG DNA methylation in Arabidopsis.