Chromatin & Epigenetics
C-terminal binding protein 2 interacts with JUNB to control macrophage inflammationThis study identifies CTBP2 as an oligomerization-dependent bridge between pro-inflammatory transcription factors and co-repressors that balances the expression of inflammatory genes.
DNA methylation predicts infection risk in kidney transplant recipientsThis study analyzed DNA methylation perturbations in patients after kidney transplantation, revealing the infection risk can be predicted using DNA methylation profiles.
Single-molecule tracking reveals the dynamic turnover of Ipl1 at the kinetochores in Saccharomyces cerevisiaeUnlike ensemble averaging methods, this study visualizes the single molecules of Ipl1 in live yeast cells to quantify its recruitment dynamics and assembly as a part of the CPC at the kinetochores.
Predicting cell cycle stage from 3D single-cell nuclear-stained imagesCellCycleNet simplifies cell cycle staging from microscopy images using only a nuclear stain (DAPI). Trained on Fucci2a reporter data, our 3D deep learning model achieves high classification accuracy (AUROC = 0.95), providing a cost-effective, intervention-free tool for cell cycle profiling.
Canonical and non-canonical PRC1 differentially contribute to regulation of neural stem cell fateEpigenetic regulation affects the capacity of stem cells to proliferate and differentiate, with canonical PRC1 being particularly important for neural stem cell fate.
PICH impacts the spindle assembly checkpoint via its DNA translocase and SUMO-interaction activitiesGenome-edited cell lines for PICH depletion or replacement to loss of function mutants revealed an unidentified PICH function impacting SAC activity through modulating Bub1 association at kinetochores.
High-resolution analysis of human centromeric chromatinCentromeres contain two physically distinct CENP-A nucleosomes, one of which associates with the CENP-C complex, and regulating CENP-C levels is critical for accessibility of the centromeric chromatin fiber for transcriptional machinery.
KAT6B is required for histone 3 lysine 9 acetylation and SOX gene expression in the developing brainHeterozygous mutations in the MYST family histone lysine acetyltransferase, KAT6B, cause intellectual disability disorders. This study identifies the histone and gene targets of KAT6B which include SOX2, a master regulator of neural stem and progenitor cells.
Chromosome-specific barcode system with centromeric repeat in cultivated soybean and wild progenitorA centromeric satellite DNA forms a higher order repeat structure for each chromosome in both cultivated and wild soybean genomes. This unique system provides a chromosome barcode and glimpses centromere conservation during domestication and breeding.
Nanoscale imaging of DNA-RNA identifies transcriptional plasticity at heterochromatinImaging mass spectrometry quantifies spatial relationships between DNA and newly formed RNA in the nucleus and illuminates transcription plasticity at condensed DNA.