Chromatin & Epigenetics
2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibitionThis study investigates the genome-wide effects of 2-HG accumulation on chromatin and gene expression and identifies Rph1-dependent and H3K36me3-associated gene repression at silenced loci in yeast.
Chromatin priming elements direct tissue-specific gene activity before hematopoietic specificationThis study shows that signalling processes prime many enhancer elements at the chromatin level before the expression of their associated genes and that priming is essential for developmental stage-specific dynamic gene activation.
Intrinsic deletion at 10q23.31, including the PTEN gene locus, is aggravated upon CRISPR-Cas9–mediated genome engineering in HAP1 cells mimicking cancer profilesThe unintended 10q23.31 deletion frequency rises in HAP1 cells stressed during CRISPR-Cas9 engineering, leading to aberrant molecular and cellular changes resembling common cancer patient deletions.
Transgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposureWe exposed pregnant female mice to neonicotinoid thiacloprid. We observed reproductive defects together with DNA methylation and histone H3K9me3 changes in the third-generation males.
Evolutionary insights into 3D genome organization and epigenetic landscape of Vigna mungoBy integrating SMRT long read, Hi-C, and Illumina pair-end sequencing technologies, we report a nearly complete, chromosomal-level assembly of V. mungo and characterize its 3D genome architecture.
Polycomb regulates circadian rhythms in Drosophila in clock neuronsThis study demonstrated that Pc regulates the circadian rhythm. The targeted DamID analysis identified the Pc-binding profiles in different clock neuron clusters, indicating Pc regulates circadian rhythms by specific clock genes.
Correction: Dispersal of PRC1 condensates disrupts polycomb chromatin domains and loopsEndogenous PRC1–mediated chromatin compaction and clustering of polycomb target loci are reversibly perturbed in mESCs by the addition of 1,6 hexandiol.
Cost-effective DNA methylation profiling by FML-seqA low-cost, high-throughput method for genome-wide DNA methylation profiling based on methylation-specific restriction digestion and sequencing.
RB loss sensitizes cells to replication-associated DNA damage after PARP inhibition by trappingAfter PARP inhibition by trapping, cells lacking the RB tumor suppressor experience persistent replication stress and genomic instability that compromise cell viability.
Chromatin in 3D distinguishes dMes-4/NSD and Hypb/dSet2 in protecting genes from H3K27me3 silencingHistone KMTs of H3K36me maintains euchromatin by antagonizing H3K27me3 spreading and influencing 3D genome organization at TADs boundaries, thus participating in transcriptional program maintenance.