Chromatin & Epigenetics
miR-486 is essential for muscle function and suppresses a dystrophic transcriptomeThe authors identify miR-486 as essential for normal muscle function and a driver of dystrophic transcriptomic pathways using mir-486 knockout mice and multiple sequencing platforms.
Nutrient sensitive protein O-GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesisMaternal hyperglycemia and elevated O-GlcNAc levels perturb promoter bivalency and lead to transcriptional up-regulation of neurogenic transcription factors during embryonic neurogenesis.
TET2-mediated epigenetic reprogramming of breast cancer cells impairs lysosome biogenesisTET2-mediated oxidation of 5-methylcytosine establishes an antiviral state and contributes to MYC-dependent down-regulation of genes involved in lysosome biogenesis and function in breast cancer cells.
Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assemblyHigh content image analysis, single molecule tracking, modeling, and DBA patient analysis revealed that ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assembly.
DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteinsLong-term epigenetic gene silencing can be induced independently of DNA methylation by dCas9-KRABd-MeCP2 fusion proteins.
AGO1 regulates pericentromeric regions in mouse embryonic stem cellsDepletion of AGO1 in mESCs leads to a redistribution of H3K9me3 and HP1α away from pericentromeric regions and is accompanied by an up-regulation of major satellites transcripts.
Angioplasty induces epigenomic remodeling in injured arteriesThis is the first in vivo epigenomic survey revealing genome-wide loci-specific chromatin mark redistribution after angioplasty; the underlying epigenetic regulations may inform therapeutic targeting.
Refining the domain architecture model of the replication origin firing factor Treslin/TICRRThe replication origin firing factor Treslin/TICRR comprises an essential Sld3-like core that requires the flanking Ku70-like N-terminal and the C-terminal domains for efficient origin firing.
Cis-regulatory hubs: a new 3D model of complex disease genetics with an application to schizophreniaGenes and their regulatory elements are organized in neurons within 3D networks which model functional structures and explain schizophrenia genetic etiology.
Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resectionDSB recruitment of the cohesin loader Scc2/4 relies on Tel1 and γH2A, but contrary to cohesin not on Mec1; binding emanates from the break site and depends on as well as coincides with end resection.