Cell Biology
- IKBKB reduces huntingtin aggregation by phosphorylating serine 13 via a non-canonical IKK pathway
Early exposure of RA induced altered expressions of Wnt-related genes and subsequent osteogenic differentiation in embryonic mouse craniofacial prominences in a spatiotemporal-dependent manner.
- Generating fast-twitch myotubes in vitro with an optogenetic-based, quantitative contractility assay
We here describe an in vitro contractility assay to control fiber-type composition in muscle cultures.
- HGF/c-Met/β1-integrin signalling axis induces tunneling nanotubes in A549 lung adenocarcinoma cells
Interactions between hepatocyte growth factor (HGF), the c-Met receptor, and β1-integrin induce tunneling nanotubes in A549 cells; paxillin and the downstream MAPK and PI3K pathways and the Arp2/3 complex drive HGF-induced TNT formation.
- PRMT5 triggers glucocorticoid-induced cell migration in triple-negative breast cancer
In triple-negative breast cancer, PRMT5 functions as a master transcriptional coregulator of the glucocorticoid receptor regulating cell migration after dexamethasone treatment. PRMT5 acts in this case as a scaffolding protein independently of its catalytic activity to promote HP1g recruitment.
- Basonuclin-2 regulates extracellular matrix production and degradation
Basonuclin-2 (BNC2) is identified as a novel regulator of genes associated with the extracellular matrix and cytokines in fibroblasts. BNC2 is thereby identified as a novel regulator of the tumour microenvironment where its high expression correlates with poor prognosis.
- Dissecting morphogenetic apoptosis through a genetic screen in Drosophila
This genetic screen identifies elements of the “morphogenetic apoptosis pathway” potentially involved in the regulation, the orchestration or the impact of the apoptotic process on morphogenesis.
- Human TLR8 induces inflammatory bone marrow erythromyeloblastic islands and anemia in SLE-prone mice
Human TLR8 induces an inflammatory phenotype in BM erythroblastic island central macrophages of lupus-prone Sle1.Yaa mice. Failure to support erythroid precursor adhesion and development induces fatal anemia caused by bone marrow failure followed by hemophagocytosis during stress erythropoiesis.
- Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesions
Using fluorescence polarisation microscopy and computational modeling, we find that FA molecules exhibit ECM dependent orientational co-alignment or order. This molecular organizational property in addition to nanoscale localization may explain precise and sensitive decoding of ECM cues.
- The C-terminal tail of CSNAP attenuates the CSN complex
The study demonstrates inhibition of the COP9 signalosome (CSN) complex by a peptide derived from CSNAP, the smallest CSN subunit. The peptide displaces the endogenous CSNAP subunit from the complex, leading to a CSNAP null phenotype that attenuates CSN activity.
- The pyruvate dehydrogenase complex regulates mitophagic trafficking and protein phosphorylation
Mutations in the PDC affect the phosphorylation and mitophagic trafficking of matrix proteins, through the novel regulation of associated kinases and a phosphatase. We suggest that this occurs by the direct allosteric regulation of the phosphatase and kinases by the PDC.