Cell Biology
Atomic force microscopy reveals structural variability amongst nuclear pore complexesStructural heterogeneity is resolved in isolated nuclear envelopes, revealing the lamina network and nuclear pore complexes of different sizes, as well as different morphologies in their transport barrier and different binding of nuclear transport receptors in the barrier.
Neuron-specific inactivation of Wt1 alters locomotion in mice and changes interneuron composition in the spinal cordThis work demonstrates a role for the Wilms tumor protein Wt1 in the specification of neurons that are involved in the control of locomotion.
Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisionsThis study reveals that mesenchymal stem cell–like dermal pericytes increase the number of epidermal stem and progenitor cells in human skin by promoting planar cell divisions within the proliferative compartment, without compromising skin differentiation—most likely via paracrine BMP-2 secretion.
Dynamic m6A methylation facilitates mRNA triaging to stress granulesm6A modification in the 5′ vicinity of the coding sequence of transcripts provides a selective mechanism for triaging mRNAs to stress granules and is mediated by the YTHDF3 “reader” protein.
Recruitment of ubiquitin-activating enzyme UBA1 to DNA by poly(ADP-ribose) promotes ATR signallingHuman but not yeast UBA1 binds poly(ADP-ribose) polymers via a solvent-exposed and positively charged patch. Poly(ADP-ribose) polymerase 1–dependent recruitment of UBA1 to DNA ensures ataxia-telangiectasia and RAD3-related activation.
DHX15 regulates CMTR1-dependent gene expression and cell proliferationDHX15 helicase regulates CMTR1-dependent first transcribed nucleotide ribose O-2 methylation.
Neuronal Aβ42 is enriched in small vesicles at the presynaptic side of synapsesSuper-resolution microscopy reveals that Aβ42 is mainly present at the presynaptic side of the synapse.
ERα activity depends on interaction and target site corecruitment with phosphorylated CREB1CREB1 interacts with, stimulates, and is necessary for estrogen receptor α activation by estrogen or cAMP signaling, and this cooperation may underlie their anti-apoptotic activities in breast cancer cells.
Multiple membrane extrusion sites drive megakaryocyte migration into bone marrow blood vesselsMegakaryocytes enter bone marrow sinusoids to generate platelets by extrusion of internal membrane into the plasma membrane at fusion sites rather than microtubule-driven proplatelet extension.
Serine catabolism is essential to maintain mitochondrial respiration in mammalian cellsMitochondrial respiratory complex assembly requires the one-carbon unit generated from serine catabolism.