Cell Biology
TMEM16A chloride channel does not drive mucus productionDespite being essential for airway hydration, TMEM16A is not required for mucus (MUC5AC) production. Cell proliferation is the main driver for TMEM16A up-regulation during inflammation.
Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signallingThis study provides a novel function of the bacterial lectin LecB, which dampens cell proliferation and survival by targeting the insulin-like growth factor-1 for degradation, without receptor activation.
Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and diseaseOxidative protein folding in the ER generates ROS, leading to Nrf2-dependent feedback on protein folding via ER calcium level modulation. This feedback loop is suppressed in ALS-associated mutant astrocytes but can be rescued by dimethyl fumarate.
Oct4 mediates Müller glia reprogramming and cell cycle exit during retina regeneration in zebrafishThe rapid induction of pluripotency-inducing factor Oct4 in the injured retina necessitates the de novo induction of stem cells and their subsequent cell cycle exit.
Mechanistic insights into the protective roles of polyphosphate against amyloid cytotoxicityThis study provides novel insights into the mechanisms by which presence of polyP alters the formation, structural properties, and cytotoxic effects of α-synuclein fibers.
The huntingtin inclusion is a dynamic phase-separated compartmentWhen expressed in yeast, mutant Htt-GFP forms an inclusion that is mobile, gel-like, and releases material; evidence suggests that it grows through collision and coalescence with small aggregates.
Pleiotropic requirements for human TDP-43 in the regulation of cell and organelle homeostasisComprehensive analysis of human TDP-43 knockout cells revealed that TDP-43 regulates hundreds of transcripts and is critical for maintaining the homeostasis of multiple subcellular organelles.
Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in miceMutation of three cationic amino acids previously identified as an extracellular phosphatidylserine-binding motif abrogates ADAM17 sheddase function and causes early embryonic death in mice.
Targeting plasma membrane phosphatidylserine content to inhibit oncogenic KRAS functionKRAS-dependent cancer cell growth is inhibited by disrupting phosphatidylserine transport to the plasma membrane by genetic knockdown of lipid exchangers ORP5 and ORP8 or by inhibition of PI4KIIIα.
A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cellsA new molecule induces AR sumoylation and degradation resulting in selective growth inhibition in AR-dependent prostate cancer cells, but its activity is blunted by interference with proteasomes.