Cell Biology
Epigenetic modifier balances Mapk and Wnt signalling in differentiation of goblet and Paneth cellsThe histone methyltransferase Mll1 controls intestinal secretory cell fate by promoting Wnt-driven Paneth and restricting Mapk-dependent goblet cell differentiation through regulation of Gata4/6 transcription factors
Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA bindingCas9 is sumoylated and ubiquitylated in human cells. K848 is the major SUMO2/3 modification site, but multiple lysines are ubiquitylated, precipitating proteasomal degradation. Preventing Cas9 sumoylation by K848 ablation or by pharmacologic means reduces Cas9 half-life and DNA binding ability.
Rbfox1 is required for myofibril development and maintaining fiber type–specific isoform expression in Drosophila musclesRbfox1 is required for the development of both fibrillar and tubular muscle fibers in adult Drosophila and interacts with Bruno1 to define fiber type–specific patterns of alternative splicing.
New insights into the phosphorylation of the threonine motif of the β1 integrin cytoplasmic domainThis study investigates phosphorylation of and kindlin recruitment by the double-threonine motif in the β1 integrin cytoplasmic tails.
Electron cryo-tomography structure of axonemal doublet microtubule from Tetrahymena thermophilaBy using electron cryo-tomography and subtomogram average, this study reveals the assembly of T. thermophila axonemal MT doublet in wild type and mutant, showing its structure conservation and diversity compared to other organisms.
Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicityUsing cryo-ET, cell biology, and proteomics, this study shows that aggregating proteins impair the autophagy-lysosomal pathway in neurons by sequestering a subunit of the AP-3 adaptor complex.
Loss of Nup210 results in muscle repair delays and age-associated alterations in muscle integrityThis study describes the the role of a nuclear pore complex protein in mammalian in skeletal muscle maintenance, repair, and function.
Fms-like tyrosine kinase 3 is a regulator of the cardiac side population in miceThis work shows that Flt3 signaling regulates the composition and functionality of the cardiac side population, which could be relevant for cardiovascular homeostasis under Flt3-inhibitory therapies.
Benchmarking a highly selective USP30 inhibitor for enhancement of mitophagy and pexophagyA detailed look at the properties of a novel USP30 inhibitor applied to studies of mitophagy and pexophagy.
MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochleaThe authors show in the mouse how the auditory hair cell structural maintenance is perturbed by the inactivation of Manf and the concomitant ER stress, causing early-onset, progressive hearing loss.