Cell Biology
Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resectionDSB recruitment of the cohesin loader Scc2/4 relies on Tel1 and γH2A, but contrary to cohesin not on Mec1; binding emanates from the break site and depends on as well as coincides with end resection.
Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sortingSmall disulfide loops of several peptide hormones are shown to promote aggregation of reporter proteins in cultured cells and to act as novel signals for sorting into secretory granules by self-aggregation.
Nucleotide biosynthesis links glutathione metabolism to ferroptosis sensitivityThe tumor suppressor protein p53 inhibits ferroptosis by reducing the consumption of glutathione in nucleotide biosynthesis.
Phenylbutyrate rescues the transport defect of the Sec61α mutations V67G and T185A for reninUsing cellular models of the ADTKD–SEC61A1 causing mutations V67G and T185A, this study reveals specific aberrations of protein transport and calcium homeostasis as a potential pathogenic mechanism.
Epigenetic modifier balances Mapk and Wnt signalling in differentiation of goblet and Paneth cellsThe histone methyltransferase Mll1 controls intestinal secretory cell fate by promoting Wnt-driven Paneth and restricting Mapk-dependent goblet cell differentiation through regulation of Gata4/6 transcription factors
Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA bindingCas9 is sumoylated and ubiquitylated in human cells. K848 is the major SUMO2/3 modification site, but multiple lysines are ubiquitylated, precipitating proteasomal degradation. Preventing Cas9 sumoylation by K848 ablation or by pharmacologic means reduces Cas9 half-life and DNA binding ability.
Rbfox1 is required for myofibril development and maintaining fiber type–specific isoform expression in Drosophila musclesRbfox1 is required for the development of both fibrillar and tubular muscle fibers in adult Drosophila and interacts with Bruno1 to define fiber type–specific patterns of alternative splicing.
New insights into the phosphorylation of the threonine motif of the β1 integrin cytoplasmic domainThis study investigates phosphorylation of and kindlin recruitment by the double-threonine motif in the β1 integrin cytoplasmic tails.
Electron cryo-tomography structure of axonemal doublet microtubule from Tetrahymena thermophilaBy using electron cryo-tomography and subtomogram average, this study reveals the assembly of T. thermophila axonemal MT doublet in wild type and mutant, showing its structure conservation and diversity compared to other organisms.
Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicityUsing cryo-ET, cell biology, and proteomics, this study shows that aggregating proteins impair the autophagy-lysosomal pathway in neurons by sequestering a subunit of the AP-3 adaptor complex.