Cell Biology
A novel domain within the CIL regulates egress of IFITM3 from the Golgi and reveals a regulatory role of IFITM3 on the secretory pathwayIn this work, it is revealed that the broad viral inhibitor IFITM3 is a previously unrecognized modulator of the functionalities of the cellular ER–Golgi secretory pathway.
Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombeCharacterization of nuclear pores in Schizosaccharomyces pombe identifies regions of heterogeneous NPC density and composition and shows that NPCs are excluded near the spindle pole body by Lem2-mediated centromere tethering.
ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathwayESCRT-I deficiency impairs lysosome membrane turnover and induces homeostatic responses to lysosomal nutrient starvation including activation of MiT-TFE signaling caused by inhibition of the substrate-specific mTORC1 pathway.
Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodiesNanobody toolkit enables the quantitative analysis of endosome-to-TGN transport of the mannose-6-phosphate receptor in cells depleted of retrograde transport machineries
Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivoCell division completes when the two daughter cells move their oldest centrosome towards the cytokinetic bridge, which is then cleaved during abscission. The GTPase, Rab11, and the centrosome protein, Pericentrin, work together to coordinate this movement.
Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastomaSpatial proteomics identifies MAP4K4 as a regulator of the plasma membrane-associated proteome and reveals CD155 and EndoA1 as mediators of proliferation and invasiveness in medulloblastoma cells.
Single serine on TSC2 exerts biased control over mTORC1 activation mediated by ERK1/2 but not AktBoth ERK1/2 and Akt kinases activate mTORC1, but only the former is bidirectionally regulated by the status of serine S1364 on TSC2 that confers input-selective mTORC1 amplification or attenuation.
DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteinsLong-term epigenetic gene silencing can be induced independently of DNA methylation by dCas9-KRABd-MeCP2 fusion proteins.
Septins tune lipid kinase activity and PI(4,5)P2 turnover during G-protein–coupled PLC signalling in vivoIn Drosophila photoreceptors, PI(4,5)P2 synthesis during PLC signalling is regulated by PIP5K and septins.
LRR-protein RNH1 dampens the inflammasome activation and is associated with COVID-19 severityRNH1 prevents inflammation by inhibiting inflammasome activation through controlling caspase-1 protein levels. In COVID-19 patients, RNH1 expression levels were negatively associated with disease severity and inflammation, suggesting a role for RNH1 in SARS-CoV-2–mediated inflammation and pathology.