Cell Biology
A dominant negative mitofusin causes mitochondrial perinuclear clusters because of aberrant tetheringOur analysis of a CMT2A-associated mitofusin variant reveals that aberrant mitochondrial tethering results in perinuclear clusters and that conformational dynamics in Hinge 2 are required to progress from membrane tethering to membrane fusion.
Non-canonical miRNA-RNA base-pairing impedes tumor suppressor activity of miR-16In uveal melanoma tumors, the RNA decay activity of the tumor suppressor miR-16 is impaired by sponge RNAs. These RNAs defined a powerful signature to predict overall survival.
Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar systemElectron tomography reveals four classes of cytopathic vesicles-II (CPV-II) stemming from the host Golgi apparatus after Venezuelan equine encephalitis virus infection.
ALS-linked loss of Cyclin-F function affects HSP90Analysis of ALS patient cell lines and cyclin-F overexpression and knockout cells identified HSP90AB1 as novel SCFcyclin-F substrate pointing to a loss-of-function mechanism for ALS CCNF mutations.
Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictionsThe authors present a CRISPRa-based gain-of-function approach to systematically uncover solute carriers sustaining cellular fitness upon limiting amino acid concentrations.
Mitochondrial impairment and intracellular reactive oxygen species alter primary cilia morphologyThis work shows how altering energetic status and promoting intracellular/mitochondrial ROS induces cell-dependent ciliary impairments, which is relevant in diseases characterized by these features.
α-Actinin-4 recruits Shp2 into focal adhesions to potentiate ROCK2 activation in podocytesα-Actinin-4 is crucial in the regulation of Shp2 FA targeting to enhance ROCK2-mediated actomyosin contractility and thereby strengthening cell adhesion and cytoskeletal architecture in podocytes.
Emetine blocks DNA replication via proteosynthesis inhibition not by targeting Okazaki fragmentsThis study clarifies the long-standing misuse of emetine as a lagging strand synthesis-specific inhibitor. Emetine blocks the synthesis of both DNA strands by the inhibition of protein biosynthesis.
Methionine uptake via the SLC43A2 transporter is essential for regulatory T-cell survivalRegulatory T cells survive after IL-2 withdrawal by taking up and using methionine through the SLC43A2 transporter in a Notch1-dependent manner.
Proteasomal down-regulation of the proapoptotic MST2 pathway contributes to BRAF inhibitor resistance in melanomaThe loss of MST2 pathway protein expression in BRAF inhibitor resistant melanoma cells is due to ubiquitination and subsequent proteasomal degradation and prevents MST2-mediated apoptosis.