Cell Biology
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents usedMono(ADP-ribosyl)ation is an emerging modification, for which recently detection reagents were generated. We cross-validated all reagents and observe remarkable differences in efficiency, depending on the substrate investigated and on the methods that are used.
Lipid saturation induces degradation of squalene epoxidase for sterol homeostasis and cell survivalThe authors describe the ER-associated degradation of Erg1 under increased membrane lipid saturation and show its importance in balancing sterol levels for membrane integrity in budding yeast.
Lack of peroxisomal catalase affects heat shock response in Caenorhabditis elegansThe authors present a previously unknown crosstalk between peroxisomes and the heat shock response, whereby the peroxisomal catalase is vital for cellular capacity to survive heat stress and undergo heat-induced lifespan extension.
Tanshinone functions as a coenzyme that confers gain of function of NQO1 to suppress ferroptosisNQO1 catalyzes the reduction of tanshinones to detoxify lipid peroxyl radicals and inhibit ferroptosis both in vitro and in vivo, providing a molecular mechanism for the therapeutic effects of tanshinones, such as cardiac protection.
CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tauUsing a genome-wide CRISPRi screen, we identified ANKLE2, BANF1, NUSAP1, EIF1AD, and VPS18 as novel regulators of tau pathology induced by both exosome-like extracellular vesicles and free tau seeds.
Miro GTPase domains regulate the assembly of the mitochondrial motor–adaptor complexRelocalizing the mitochondrial motor–adaptor protein Miro to peroxisomes and systematically manipulating each GTPase domain reveal the importance of the N-terminal GTPase domain of Miro1 for regulating mitochondrial transport.
circEXOC5 promotes acute lung injury through the PTBP1/Skp2/Runx2 axis to activate autophagycircEXOC5 activates autophagy and promotes acute lung injury via stimulating the Skp2 decay to stabilize Runx2 by interacting with PTBP1.
Stepwise progression of β-selection during T cell development involves histone deacetylationT cells undergoing beta-selection differentiate via sequential expression of co-receptors CD28, CD5, and CD2, exposing new signalling controls that are disrupted by a cancer therapeutic.
A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biologyThe authors describe a high-throughput method for measuring endogenous GLUT4 and transferrin receptor translocation in adipocytes. They reveal that this method has advantages over studying GLUT4 trafficking using overexpressed GLUT4 reporters, which are commonly used in the field.
MDF is a conserved splicing factor and modulates cell division and stress response in ArabidopsisMDF acts like its human homolog SART1 as a splicing factor that is important for maintenance of genome stability and correct splicing and expression of genes involved in cell division and stress response.