Cell Biology
- ERK2 MAP kinase regulates SUFU binding by multisite phosphorylation of GLI1
As a mechanism of crosstalk, potentially relevant to cancer and developmental signaling, ERK2 MAP kinase phosphorylates the Hedgehog-pathway transcription factor GLI1 on three sites, promoting release of the negative regulator SUFU and the consequent activation of GLI1.
- PVT1 is a stress-responsive lncRNA that drives ovarian cancer metastasis and chemoresistance
PVT1 is a YAP1 dependent stress responsive lncRNA that promotes ovarian cancer metastasis and chemoresistance, making PVT1 a promising therapeutic target.
- KIF24 depletion induces clustering of supernumerary centrosomes in PDAC cells
Depletion of the centrosomal kinesin KIF24, known to restrain the assembly of primary cilia, suppresses multipolar spindle formation by clustering centrosomes in centrosome-amplified PDAC cells.
- Identification and characterization of a membrane receptor that binds to human STC1
A study using TriCEPS-based ligand–receptor methodology and surface plasmon resonance assays identified that human stanniocalcin-1 binds to insulin-like growth factor-2 receptors in human leukemia monocytic cells with high affinity.
- Proteins implicated in muscular dystrophy and cancer are functional constituents of the centrosome
This study demonstrates that the muscular dystrophy-associated proteins dystrophin, utrophin, dysferlin, and calpain-3 localize to the centrosome and that their absence leads to excess centrosomes, compromised nuclear morphology, impaired centrosome orientation, and defective microtubule nucleation.
- Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD–associated UBQLN2 mutants
Analysis of ALS patient-derived and engineered cells revealed that mutant UBQLN2 increases mRNA and protein of MAP1B which is mediated by dephosphorylation of FUS within its RNA-binding domain.
- Loss of Amphiregulin drives inflammation and endothelial apoptosis in pulmonary hypertension
The key pathogenic features of pulmonary hypertension are endothelial apoptosis and vascular inflammation, which are caused by the down-regulation of Amphiregulin, accompanied by HIF-1a and BAD-mediated up-regulation of the target genes such as RRP1B, PHB2, and NCOA6.
- TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathy
TMBIM5 deficiency reduces mitochondrial K+/H+ exchange. Mutation of the channel pore in mice destabilizes the protein and results in increased embryonic lethality and a skeletal myopathy.
- PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous system
PRMT1 regulates MHC-associated microglia cluster during de/remyelination.
- The cation exchanger Letm1, circadian rhythms, and NAD(H) levels interconnect in diurnal zebrafish
The mitochondrial osmoregulator Letm1 exhibits a diel regulation, impacts on NAD+/H levels, circadian genes, and its study allows new perspectives on possible genetic compensation mechanisms.