Cell Biology
ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathwayESCRT-I deficiency impairs lysosome membrane turnover and induces homeostatic responses to lysosomal nutrient starvation including activation of MiT-TFE signaling caused by inhibition of the substrate-specific mTORC1 pathway.
Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodiesNanobody toolkit enables the quantitative analysis of endosome-to-TGN transport of the mannose-6-phosphate receptor in cells depleted of retrograde transport machineries
Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivoCell division completes when the two daughter cells move their oldest centrosome towards the cytokinetic bridge, which is then cleaved during abscission. The GTPase, Rab11, and the centrosome protein, Pericentrin, work together to coordinate this movement.
Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastomaSpatial proteomics identifies MAP4K4 as a regulator of the plasma membrane-associated proteome and reveals CD155 and EndoA1 as mediators of proliferation and invasiveness in medulloblastoma cells.
Single serine on TSC2 exerts biased control over mTORC1 activation mediated by ERK1/2 but not AktBoth ERK1/2 and Akt kinases activate mTORC1, but only the former is bidirectionally regulated by the status of serine S1364 on TSC2 that confers input-selective mTORC1 amplification or attenuation.
DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteinsLong-term epigenetic gene silencing can be induced independently of DNA methylation by dCas9-KRABd-MeCP2 fusion proteins.
Septins tune lipid kinase activity and PI(4,5)P2 turnover during G-protein–coupled PLC signalling in vivoIn Drosophila photoreceptors, PI(4,5)P2 synthesis during PLC signalling is regulated by PIP5K and septins.
LRR-protein RNH1 dampens the inflammasome activation and is associated with COVID-19 severityRNH1 prevents inflammation by inhibiting inflammasome activation through controlling caspase-1 protein levels. In COVID-19 patients, RNH1 expression levels were negatively associated with disease severity and inflammation, suggesting a role for RNH1 in SARS-CoV-2–mediated inflammation and pathology.
Growth-rate-dependent and nutrient-specific gene expression resource allocation in fission yeastDuring growth on nitrogen sources, growth-rate-correlated gene expression is a trade-off between the stress and growth programmes, whereas medium-specific gene expression reflects metabolic states.
Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputsThe tRNA ligase RtcB is tyrosine phosphorylated upon ER stress which in turn leads to modulation of XBP1 mRNA splicing and IRE1 signaling outputs.