Cell Biology
- The histone H4K20 methyltransferase SUV4-20H1/KMT5B is required for multiciliated cell differentiation in Xenopus
Cilia tufts on Xenopus larval epidermis are generated through a burst of ciliogenic gene transcription, which depends on conversion of monomethylated to dimethylated histone H4 lysine 20 by SUV4-20H1.
- SIRT6 overexpression in the nucleus protects mouse retinal pigment epithelium from oxidative stress
Oxidative stress causes a rapid translocation of SIRT6 from the nucleus to the cytoplasm leading to nuclear SIRT6 depletion and results in RPE damage that is more severe in the central region in mice.
- The C-type lectin domain of CD62P (P-selectin) functions as an integrin ligand
The lectin domain of CD62P specifically binds to and activates integrins. CD62P-integrin interactions may function in a diverse set of cell–cell adhesions given that integrins are widely expressed.
- The cytochrome b carboxyl terminal region is necessary for mitochondrial complex III assembly
Synthesis/assembly of Cytb, encoded in the mitochondrial DNA, are regulated by the Cytb C-terminus. It modifies the interaction Cytb-Cbp3/Cbp6 to control Cytb and respiratory complex III biogenesis.
- Cognitive decline in diabetic mice predisposed to Alzheimer’s disease is greater than in wild type
This work proposes that T2DM is particularly harmful to brain function in individuals with a genetic predisposition to neurodegenerative pathology via, among others, γ-secretase inhibition.
- The ubiquitin-like modifier FAT10 is degraded by the 20S proteasome in vitro but not in cellulo
The ubiquitin-like modifier FAT10 is degraded by the 20S proteasome in vitro, whereas FAT10 degradation depends on the 26S proteasome in cellulo, shown by impairing 26S function via Rpt2 knockdown.
- UPF3A is dispensable for nonsense-mediated mRNA decay in mouse pluripotent and somatic cells
We generated a new Upf3a-inducible knockout mouse model and showed that UPF3A does not repress expressions of NMD target genes in embryonic stem cells, somatic fibroblasts, and multiple tissues from adult mice.
- The association of lipid transfer protein VPS13A with endosomes is mediated by sorting nexin SNX5
Binding of sorting nexin SNX5 to the VAB domain of VPS13A mediates its recruitment to membrane contact sites between SNX5-containing endosomal membranes, mitochondria, and ER.
- The role of the mitochondrial outer membrane protein SLC25A46 in mitochondrial fission and fusion
The mitochondrial outer membrane protein SLC25A46 localizes to mitochondrial fusion and fission sites, and loss of function alters mitochondrial morphology and oligomerization states of MFN2 and OPA1.
- The role of Limch1 alternative splicing in skeletal muscle function
This works identifies a novel, alternatively spliced isoform of LIMCH1, that is, developmentally regulated and specific to skeletal muscle. Knocking out the six skeletal muscle–specific exons of LIMCH1 leads to impaired skeletal muscle function in vivo and ex vivo.