Cell Biology
- Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest
Elevated Cx37 expression in endothelium stabilizes nuclear Foxo3a by sequestering active ERK in the cytoplasm, thereby up-regulating p27 expression to promote late G1 state and enable arterial gene expression.
- Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum
This study investigates the early effects of PGF2α signaling on mitochondrial dynamics and mitophagy in bovine corpora lutea. Luteolytic mediator PGF2α, via PKC/ERK and AMPK signaling, activates mitochondrial fission and promotes PINK–Parkin mitophagy, placing mitochondria as novel targets in response to PGF2α.
- Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases
This study shows that the endogenous NEK1 kinase and C21ORF2 form a tight complex in human cells and characterises the functional significance of the association of the two partner proteins.
- Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope
The LINC complex tethers the cell nucleus to the cytoskeleton to regulate mechanical forces during cell migration, differentiation, and various diseases. We provide evidence for SUN2 disulfide bond rearrangement as a physiologically relevant structural modification that regulates LINC complex functions.
- FAT10 and NUB1L cooperate to activate the 26S proteasome
26S proteasome activation is achieved by the collective binding of the ubiquitin-like modifier FAT10 and its interaction partner NUB1L, causing gate opening in a USP14-independent way.
- Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network
The site-specific function of EGFR’s C-terminal tyrosines is interrogated by phosphotyrosine phosphoproteomics to build a data-driven model that links cell response to signaling nodes and elucidates novel associations in the EGFR signaling network.
- Cohesin SMC1β promotes closed chromatin and controls TERRA expression at spermatocyte telomeres
Cohesin SMC1β; ensures silencing at chromosome ends, restricts TERRA expression and RNA-DNA hybrid formation, and prevents telomer damage.
- Activation of XBP1 but not ATF6α rescues heart failure induced by persistent ER stress in medaka fish
Accumulation of misfolded proteins in the ER (ER stress) causes the development and progression of various diseases, and fully activated XBP1, a transcription factor downstream of IRE1 in the unfolded protein response, cures persistent ER stress–induced heart failure in medaka.
- Vitamin C protects retinal ganglion cells via SPP1 in glaucoma and after optic nerve damage
Vitamin C protects retinal ganglion cells in animal models of optic nerve injury partially by up-regulating the expression of the neuroprotective signaling molecule SPP1.
- BiFCo: visualizing cohesin assembly/disassembly cycle in living cells
A novel strategy developed in fission yeast to visualise only-assembled cohesin complex loading/unloading cycles in eukaryotic chromosomes in living cells.