Cell Biology
Quantification of cristae architecture reveals time-dependent characteristics of individual mitochondriaA novel, machine-learning approach to quantify cristae density and architecture in live-cell super-resolution imaging characterizes the ultrastructural parameters of individual mitochondria over time.
Unbiased yeast screens identify cellular pathways affected in Niemann–Pick disease type CThree independent yeast genetic screens were performed identifying proteins and pathways contributing to the pathogenesis of NPC disease and suggesting new therapeutic avenues.
Genetic targeting of neurogenic precursors in the adult forebrain ventricular epitheliumIn vivo evidence for precursors that produce neurons independent of neurosphere-forming neural stem cells suggests the adult forebrain, like the developing brain, has two distinct neurogenic pathways.
STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancersThis work highlights the degradation of STAG1 and inhibition of its interaction with cohesin as promising therapeutic strategies for exploiting synthetic lethality to treat STAG2-mutated tumors and lays the groundwork for the discovery of STAG1-directed small molecules.
Lipid-associated PML structures assemble nuclear lipid droplets containing CCTα and Lipin1PML proteins assemble into noncanonical lipid-associated PML structures (LAPS) on nuclear lipid droplets, which recruit CCTα and Lipin1 for the synthesis of phosphatidylcholine and triacylglycerol.
Importance of γ-secretase in the regulation of liver X receptor and cellular lipid metabolismInhibition of the Alzheimer associated γ-secretase impairs the regulation of cellular lipid droplet homeostasis.
Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMDCaspase-1 activation in GSDMD-deficient cells induces a rapid form of caspase-3–dependent secondary necrosis that is licenced by caspase-1–induced Bid cleavage and the release of mitochondrial SMAC.
Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesisThe hereditary spastic paraplegia protein spastin limits the biogenesis of lipid droplets at the endoplasmic reticulum in a microtubule-independent manner, whereas it promotes lipid droplet movement by binding to the microtubules.
The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cellsAnalysis of the role of the molecular clock protein Bmal1 in pluripotent embryonic stem cells reveals a novel function of circadian proteins in cell differentiation and early embryo development.
Defective nucleotide-dependent assembly and membrane fusion in Mfn2 CMT2A variants improved by BaxMfn2 CMT2A–associated variants located proximal to the hinge connecting its two extended helical bundles have impaired GTP-dependent assembly and mitochondrial fusion activity, which are both improved by cytosolic Bax.