Cell Biology
Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibitionChromosomal instability (CIN) is a hallmark feature of cancer cells. In this study, Schukken and colleagues screen for compounds that selectively target CIN cells and identify an inhibitor of Src kinase to be selectively toxic for CIN cells.
Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodelingThis study highlights the role of Nup93-chromatin interactions in driving triple-negative breast cancer propagation through modulation of the actin cytoskeleton, cell migration and proliferation.
Laminin is the ECM niche for trophoblast stem cellsLaminin functions as an ECM niche factor for trophoblast stem cells and secures trophoblast stem cell expansion through its interactions with integrin.
Oscillatory cAMP signaling rapidly alters H3K4 methylationThis work explores how GPCR-cAMP signaling dynamically influences histone methylation by altering intracellular labile Fe(II) levels and subsequently modulating histone demethylase activity.
CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesisCLIC members are required for the progression of cytokinesis by coupling the plasma membrane and cortical actin network at the cleavage furrow and polar cortex.
Dual role of a GTPase conformational switch for membrane fusion by mitofusin ubiquitylationMitochondrial fusion requires a conformational change in α4 of the GTPase domain, followed by AAA-ATPase regulation, thus revealing striking mechanistic similarities between small and large GTPases.
MERLIN: a novel BRET-based proximity biosensor for studying mitochondria–ER contact sitesMERLIN is a novel biosensor that generates a BRET signal with a signal-to-noise ratio that is sufficient to enable sensing the proximity between the mitochondria and the ER without forcing interaction or establishing artificial connections at the MERCs.
TspanC8 tetraspanins differentially regulate ADAM10 endocytosis and half-lifeThis study demonstrates that the tetraspanins Tspan5 and Tspan15 which directly associate with the metalloprotease ADAM10 have an opposite impact on its endocytosis and half-life.
ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survivalADAP1, a GTPase-activating protein (GAP) for the small GTPase ARF6, is a strong predictor of poor survival in early-stage squamous cell carcinoma patients and a critical mediator of TGF-β-induced invasive cell migration by facilitating basement membrane breakdown.
The role of MHC class I recycling and Arf6 in cross-presentation by murine dendritic cellsMHC-I internalization and recycling in DCs neither requires Arf6 nor provides MHC-I to the Rab11+ endocytic recycling compartment; however, cross-presentation of immune complexes depends on Arf6.