Cell Biology
Modeling fibrotic alveolar transitional cells with pluripotent stem cell-derived alveolar organoidsIn vitro modeling of transitional alveolar epithelial cells in idiopathic pulmonary fibrosis by the use of a fibrosis cocktail and alveolar organoids from induced pluripotent stem cells.
UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryosThis study shows that the UHRF1 protein, an epigenetic factor, regulates cytoplasmic proteins in oocytes independently of gene expression and ensures normal preimplantation development.
MAP4K3 inhibits Sirtuin-1 to repress the LKB1–AMPK pathway to promote amino acid-dependent activation of the mTORC1 complexOur results reveal the existence of a novel signaling pathway linking amino acid satiety with MAP4K3-dependent suppression of SIRT1 to inactivate the repressive LKB1–AMPK pathway and thereby potently activate the mTORC1 complex to dictate the metabolic disposition of the cell.
The CHARGE syndrome-associated protein FAM172A controls AGO2 nuclear importAGO2 has long been known to shuttle between the cytosol and the nucleus of mammalian cells and this study helps to understand how this occurs.
WDR31 displays functional redundancy with GTPase-activating proteins (GAPs) ELMOD and RP2 in regulating IFT complex and recruiting the BBSome to ciliumOur research reveals that the deletion of WDR-31, RP-2, and ELMD-1 is leading to ciliary accumulation of IFT Complex B components and KIF17 kinesin. In conclusion, WDR31, ELMOD, and RP2 are working in regulating IFT via the BBSome.
Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrestElevated Cx37 expression in endothelium stabilizes nuclear Foxo3a by sequestering active ERK in the cytoplasm, thereby up-regulating p27 expression to promote late G1 state and enable arterial gene expression.
Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteumThis study investigates the early effects of PGF2α signaling on mitochondrial dynamics and mitophagy in bovine corpora lutea. Luteolytic mediator PGF2α, via PKC/ERK and AMPK signaling, activates mitochondrial fission and promotes PINK–Parkin mitophagy, placing mitochondria as novel targets in response to PGF2α.
Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseasesThis study shows that the endogenous NEK1 kinase and C21ORF2 form a tight complex in human cells and characterises the functional significance of the association of the two partner proteins.
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelopeThe LINC complex tethers the cell nucleus to the cytoskeleton to regulate mechanical forces during cell migration, differentiation, and various diseases. We provide evidence for SUN2 disulfide bond rearrangement as a physiologically relevant structural modification that regulates LINC complex functions.
FAT10 and NUB1L cooperate to activate the 26S proteasome26S proteasome activation is achieved by the collective binding of the ubiquitin-like modifier FAT10 and its interaction partner NUB1L, causing gate opening in a USP14-independent way.