Cell Biology
INPP5K and Atlastin-1 maintain the nonuniform distribution of ER–plasma membrane contacts in neuronsCIL-1 and ATLN-1 maintain the balance between ER tubules and sheets and prevent invasion of cortical ER sheets into the axon, contributing to the non-uniform distribution of neuronal ER-PM contacts.
Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in miceOptogenetic activation of FGFR2 allowed temporally precise induction of signaling and behavioural changes, but counter-regulation at multiple levels prevented a sustained response in keratinocytes.
An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasitesMalaria arises during the proliferation of Plasmodium spp. in human blood, whereas the underlying atypical cell division mechanisms remain poorly understood. This study uses advanced imaging to dissect dynamics and organization of the centrosome, a key division regulator.
High levels of TFAM repress mammalian mitochondrial DNA transcription in vivoThe TFAM-to-mtDNA ratio is critically important and must be maintained within a physiological range to allow normal mtDNA expression and prevent nucleoid hypercompaction in different mouse tissues.
Elevated glucose increases genomic instability by inhibiting nucleotide excision repairExposure to chronic, elevated glucose inhibits nucleotide excision repair, which leads to accumulation of DNA glycation adducts, increased DNA strand breaks, and activation of the DNA damage response.
A role for Rad5 in ribonucleoside monophosphate (rNMP) toleranceRibonucleoside incorporation in genomic DNA poses a significant threat to genomic integrity. Here, we describe how cells tolerate this threat and discuss implications for cancer therapeutics.
AMOTL2 mono-ubiquitination by WWP1 promotes contact inhibition by facilitating LATS activationThis work reveals a novel function of WWP1 E3 ligase in the mono-ubiquitination of AMOTL2, which enables the binding and activation of LATS kinases upon contact inhibition.
Tyrosine phosphorylation of lamin A by Src promotes disassembly of nuclear lamina in interphasePhosphorylation of lamin A at tyrosine residue 45 by Src has an adverse effect on the assembly of nuclear lamina, which may be involved in nuclear dysmorphia, genomic instability, and nuclear plasticity in interphase.
Extracellular matrix mediates moruloid-blastuloid morphodynamics in malignant ovarian spheroidsExpression dynamics of basement membrane-like extracellular matrix and fibronectin regulate transitions between solid (moruloid) and cavitational (blastuloid) phenotypes of ovarian cancer spheroids.
Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cellsUsing genetically encoded Förster resonance energy transfer calcium sensors for the peroxisome, this study dissects the previously contentious peroxisome involvement in calcium handling in HeLa cells and in cardiomyocytes derived from human induced pluripotent stem cells.