Cell Biology
ER targeting of non-imported mitochondrial carrier proteins is dependent on the GET pathwayThe GET pathway is required to target non-imported mitochondrial carrier proteins to the endoplasmic reticulum, which prevents their deposition into Hsp42-dependent protein foci.
Reduced RNA turnover as a driver of cellular senescenceRNAs originating from transcription upstream and downstream of genes accumulate in the cytoplasm of a subset of senescent cells, suggesting an RNA alternative to cytoplasmic DNA in the triggering of senescence.
Cyclin A2 localises in the cytoplasm at the S/G2 transition to activate PLK1Main mitotic kinases as PLK1 are activated at the S/G2 transition. A change in Cyclin A2 localisation at the S/G2 transition enables activation of PLK1.
HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tailsCiliary accumulation of G protein–coupled receptors HTR6 and SSTR3 depends on redundant ciliary targeting sequences acting via ciliary trafficking adapters TULP3 and RABL2.
FAM83F regulates canonical Wnt signalling through an interaction with CK1αFAM83F directs CK1α to the plasma membrane, and through its association with CK1α, FAM83F mediates canonical Wnt signalling.
IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signallingIMiDs induce the degradation of the FAM83F–CK1α complex but no other FAM83–CK1α complexes, resulting in attenuation of Wnt signalling.
Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4Differentiated dendritic cells display an unusual activation of the integrated stress response, which is necessary for normal type-I Interferon production and cell migration.
Degradation of arouser by endosomal microautophagy is essential for adaptation to starvation in DrosophilaDrosophila EPS8-family protein Arouser is constitutively degraded by endosomal microautophagy; its stabilisation upon starvation is essential to the animal adaptation and survival.
SCFCdc4 ubiquitin ligase regulates synaptonemal complex formation during meiosisDuring meiosis, homologous chromosomes pair to form the synaptonemal complex (SC). This study showed that SCFCdc4 ubiquitin ligase is required for and works with Pch2 AAA+ ATPase for SC assembly.
ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomesA simple and efficient recombineering-based method for introducing point mutations into bacterial artificial chromosomes using an artificial intron cassette.