Biophysics
Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesionsUsing fluorescence polarisation microscopy and computational modeling, we find that FA molecules exhibit ECM dependent orientational co-alignment or order. This molecular organizational property in addition to nanoscale localization may explain precise and sensitive decoding of ECM cues.
Clec12a inhibits MSU-induced immune activation through lipid raft expulsionC type lection receptor Clec12a inhibits MSU induced Dendritic cell activation via its transmembrane domain rather than intracellular ITIM.
Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generationThis article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endD2 region in CAMSAP3 preferentially bound to expanded microtubule lattices and also induced lattice expansion, explaining the molecular functions of CAMSAP3.
Linear ubiquitination induces NEMO phase separation to activate NF-κB signalingThe E3 ubiquitin ligase HOIP generates linear ubiquitin chains that promote liquid-liquid phase separation of NEMO in vitro, which correlates with the formation of NEMO condensates and IKK complex activation in cells.
Membrane cholesterol regulates inhibition and substrate transport by the glycine transporter, GlyT2Cholesterol binds to the GlyT2 SLC6 transporter, enhancing the effect of bioactive lipid analgesics, providing new therapeutic avenues to treat neuropathic pain and target SLC6 transporters.
Structural and functional characterization of TraI from pKM101 reveals basis for DNA processingCharacterization of relaxase TraI from pKM101 highlights large conformational changes upon DNA binding, and unlike previous reports shows similar behavior as its homologs from the R388 and F plasmids.
Actin-binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIRng2CHD, an actin-binding domain of an IQGAP, induces cooperative conformational changes in actin filaments and inhibits movement driven by myosin II.
An inducible amphipathic α-helix mediates subcellular targeting and membrane binding of RPE65Amino acid residues 107–125 of RPE65, in concert with a palmitoyl group at C112, form a membrane-sensing amphipathic helix that targets RPE65 to smooth endoplasmic reticulum to acquire its substrate.
Binding stoichiometry and structural model of the HIV-1 Rev/importin β complexBiochemical, biophysical, and docking studies provide insights into how the HIV-1 regulatory protein Rev is recognized by the nuclear import factor importin beta.