Biophysics
Exploring the molecular composition of the multipass translocon in its native membrane environmentCryo-ET reveals the compositional variability of the multipass translocon complex dependent on translational activity of the bound ribosome and suggests the location of its subunit NOMO.
The F-actin bundler SWAP-70 promotes tumor metastasisCell culture, and biophysical and mouse data show that the F-actin modulator protein SWAP-70 supports tumor cell metastasis.
Molecular mechanism for recognition of the cargo adapter Rab6GTP by the dynein adapter BicD2We present a structural model for recognition of a key modulator of protein secretion, Rab6GTP, by the dynein adapter BicD2, which provides new insights into the trafficking of secretory vesicles.
SIRT4 as a novel interactor and candidate suppressor of C-RAF kinase in MAPK signalingThis study proposed a novel extra-mitochondrial role of the tumor suppressor SIRT4 in negatively regulating the MAPK pathway by interacting with C-RAF kinase.
Breast implant surface topography triggers a chronic-like inflammatory responseThis study explores the impact of breast implant surface topographies on immune responses, using analyses of periprosthetic fluids and in vitro cultures on model surface replicas, revealing that macrotextured implants significantly induce chronic-like inflammatory reactions.
Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographyCryo-electron tomography and subtomogram averaging reveal new structural features at the base of the light-sensing cilia of retinal rods, including the basal body, the Y-links between axoneme and membrane, and the ciliary necklace of the transition zone.
Interconnections between m6A RNA modification, RNA structure, and protein–RNA complex assemblyThis review summarizes current knowledge and future directions on the mechanisms that govern the interplay between m6A RNA modification, RNA folding, and protein–RNA interactions, focusing on the mechanisms and available quantitative information.
Passive diffusion accounts for the majority of intracellular nanovesicle transportIn contrast to large vesicles and organelles that are moved by motors inside cells, a detailed analysis of intracellular vesicle mobility reveals that passive diffusion is the main mode of small vesicle transport.
LEF1 enhances β-catenin transactivation through IDR-dependent liquid–liquid phase separationThis research elucidates the correlation between liquid–liquid phase separation (LLPS) of LEF1 with β-catenin and their transcriptional activation function, offering a novel therapeutic approach for the treatment of colorectal cancer through LLPS.
ATP-independent substrate recruitment to proteasomal degradation in mycobacteriaMycobacterial proteasomes ensure survival during stress by recruiting substrates for degradation via ring-shaped activators. This study elucidates the mechanism of ATP-independent substrate recruitment through the bacterial proteasome activator (Bpa), revealing the determinants for substrate recognition and efficient degradation.