Biophysics
Interconnections between m6A RNA modification, RNA structure, and protein–RNA complex assemblyThis review summarizes current knowledge and future directions on the mechanisms that govern the interplay between m6A RNA modification, RNA folding, and protein–RNA interactions, focusing on the mechanisms and available quantitative information.
Passive diffusion accounts for the majority of intracellular nanovesicle transportIn contrast to large vesicles and organelles that are moved by motors inside cells, a detailed analysis of intracellular vesicle mobility reveals that passive diffusion is the main mode of small vesicle transport.
LEF1 enhances β-catenin transactivation through IDR-dependent liquid–liquid phase separationThis research elucidates the correlation between liquid–liquid phase separation (LLPS) of LEF1 with β-catenin and their transcriptional activation function, offering a novel therapeutic approach for the treatment of colorectal cancer through LLPS.
ATP-independent substrate recruitment to proteasomal degradation in mycobacteriaMycobacterial proteasomes ensure survival during stress by recruiting substrates for degradation via ring-shaped activators. This study elucidates the mechanism of ATP-independent substrate recruitment through the bacterial proteasome activator (Bpa), revealing the determinants for substrate recognition and efficient degradation.
Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesionsUsing fluorescence polarisation microscopy and computational modeling, we find that FA molecules exhibit ECM dependent orientational co-alignment or order. This molecular organizational property in addition to nanoscale localization may explain precise and sensitive decoding of ECM cues.
Clec12a inhibits MSU-induced immune activation through lipid raft expulsionC type lection receptor Clec12a inhibits MSU induced Dendritic cell activation via its transmembrane domain rather than intracellular ITIM.
Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generationThis article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endD2 region in CAMSAP3 preferentially bound to expanded microtubule lattices and also induced lattice expansion, explaining the molecular functions of CAMSAP3.
Linear ubiquitination induces NEMO phase separation to activate NF-κB signalingThe E3 ubiquitin ligase HOIP generates linear ubiquitin chains that promote liquid-liquid phase separation of NEMO in vitro, which correlates with the formation of NEMO condensates and IKK complex activation in cells.
Membrane cholesterol regulates inhibition and substrate transport by the glycine transporter, GlyT2Cholesterol binds to the GlyT2 SLC6 transporter, enhancing the effect of bioactive lipid analgesics, providing new therapeutic avenues to treat neuropathic pain and target SLC6 transporters.