Biophysics
Lipid-driven Src self-association modulates its transformation capacityLipid-mediated condensates formed by Src on supported lipid membranes are dependent of a conserved lysine cluster in its SH4 domain and modulate its transforming capacity.
Mechanical forces and ligand binding modulate Pseudomonas aeruginosa PilY1 mechanosensitive proteinSingle-molecule experiments reveal that PilY1’s behavior changes with force load and ligand binding, allowing for the mechanical compartmentalization of its functions.
High-resolution analysis of human centromeric chromatinCentromeres contain two physically distinct CENP-A nucleosomes, one of which associates with the CENP-C complex, and regulating CENP-C levels is critical for accessibility of the centromeric chromatin fiber for transcriptional machinery.
Mechanical compressive forces increase PI3K output signaling in breast and pancreatic cancer cellsThis article provides a proof-of-concept showing that the therapeutic impact of targeted therapies on solid cancer cells is dependent on compressive mechanical context.
Structural and biochemical analysis of ligand binding in yeast Niemann–Pick type C1–related proteinResults of X-ray crystallography and binding assays with different lipids expand our knowledge of the substrate scope of the Niemann–Pick type C1–related proteins NCR1 and NPC2 in yeast.
Microglia are essential for tissue contraction in wound closure after brain injury in zebrafish larvaeAlthough in humans, the brain fails to heal after an injury, young zebrafish are able to restore tissue structural integrity in less than 24 h, thanks to the mechanical action of microglia.
Chaperone BiP controls ER stress sensor Ire1 through interactions with its oligomersActivation of the stress sensor Ire1 is triggered by stress-induced conformational changes in its luminal domains that promote Ire1 oligomerization. This study demonstrates that the molecular chaperone BiP controls this process by binding to and destabilizing Ire1 oligomers.
Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruziElectron microscopy analysis of the osmoregulatory system in T. cruzi cell lines subjected to cryopreparation methods revealed dynamic changes in the contractile vacuole complex (CVC) and the structure of the adhesion plaque domain during the CVC pulsation cycle.
Structure of the human systemic RNAi defective transmembrane protein 1 (hSIDT1) reveals the conformational flexibility of its lipid binding domainThis study reports a cryo-EM structure of a small non-coding RNA transporter, hSIDT1, and delineates the role of lipid in the conformational dynamics of the lipid binding domain of hSIDT1.
Calaxin is a key factor for calcium-dependent waveform control in zebrafish spermThis study demonstrates that Calaxin’s calcium-binding activity is necessary for the calcium-induced asymmetric beating of cilia but dispensable to stabilize outer arm dynein.