Biophysics
KAP1 is an antiparallel dimer with a functional asymmetryThis study reveals the architecture of human KAP1 by integrating molecular modeling with small-angle X-ray scattering and single-molecule experiments. KAP1 dimers feature a structural asymmetry at the C-terminal domains that has functional implications for recruitment of HP1.
PRDM9 forms a trimer by interactions within the zinc finger arrayPRDM9 forms a trimer as a soluble protein and in complex with DNA mediated by the ZnF domain. Five ZnFs are already sufficient for multimerization and only one DNA molecule is complexed in the trimer.
ATP hydrolysis by KaiC promotes its KaiA binding in the cyanobacterial circadian clock systemATP hydrolysis in the KaiC hexamer triggers the exposure of its C-terminal segments into the solvent so as to capture KaiA, providing mechanistic insights into the circadian periodicity regulation.
Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironmentsThis study shows the activation of tumour-associated mesenchymal stromal cells by breast cancer secretomes in bioengineered 3D microenvironments using comprehensive multiomics analysis methods.
KIF18A's neck linker permits navigation of microtubule-bound obstacles within the mitotic spindleThe length of KIF18A's neck linker permits navigation of obstacles along K-fibers within the mitotic spindle and is required for the timely alignment and segregation of mitotic chromosomes.
Secretory granule protein chromogranin B (CHGB) forms an anion channel in membranesThe CHGB subfamily of secretory granule proteins forms a new family of anion-selective channels by interacting with membranes via two amphipathic α-helices. The channel exhibits higher anion selectivity, larger conductance, higher DIDS-binding affinity, and higher Cl− sensitivity than other known anion channels.
Atomic force microscopy reveals structural variability amongst nuclear pore complexesStructural heterogeneity is resolved in isolated nuclear envelopes, revealing the lamina network and nuclear pore complexes of different sizes, as well as different morphologies in their transport barrier and different binding of nuclear transport receptors in the barrier.
Beetle luciferases with naturally red- and blue-shifted emissionNew crystal structures of red- and green blue–shifted beetle luciferases reveal that the color emission mechanism is dependent on the active site microenvironment affected by the conformation of loop regions.
Co-existing feedback loops generate tissue-specific circadian rhythmsThe analysis of tissue-specific data-based models of the gene regulatory network of the mammalian circadian clock reveals organ-specific synergies of feedback loops.
Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1The stepwise ribosome disassembly in the translation cycle of eukaryotes and archaea is scheduled by discrete molecular events within the asymmetric ribosome recycling factor ABCE1.