Most read article(s)
Decreasing pdzd8-mediated mito–ER contacts improves organismal fitness and mitigates Aβ42 toxicityThe authors provide the first description of the impact of reducing pdzd8-mediated mito–ER contacts which prolongs lifespan, reduces age-related locomotor decline, and protects against mitochondrial toxins. pdzd8 loss is also protective against neuronal toxicity from Aβ42.
ERK2 MAP kinase regulates SUFU binding by multisite phosphorylation of GLI1As a mechanism of crosstalk, potentially relevant to cancer and developmental signaling, ERK2 MAP kinase phosphorylates the Hedgehog-pathway transcription factor GLI1 on three sites, promoting release of the negative regulator SUFU and the consequent activation of GLI1.
ScRNA-seq expression of IFI27 and APOC2 identifies four alveolar macrophage superclusters in healthy BALFThere are at least 14 AM subtypes; their frequency, along with other immune cells, is highly conserved across individuals, suggesting a specific niche exists for each leukocyte population.
Heparan sulfate proteoglycans mediate prion-like α-synuclein toxicity in Parkinson’s in vivo modelsThis study offers new Caenorhabditis elegans models in which feeding with α-synuclein pre-formed fibrils results in prion-like aggregation of host α-synuclein and dopamine neuron degeneration, and these disease phenotypes are regulated by the heparan sulfate proteoglycan pathway.
Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD–associated UBQLN2 mutantsAnalysis of ALS patient-derived and engineered cells revealed that mutant UBQLN2 increases mRNA and protein of MAP1B which is mediated by dephosphorylation of FUS within its RNA-binding domain.
STAT3 in tumor fibroblasts promotes an immunosuppressive microenvironment in pancreatic cancerUsing a dual recombinase mouse model of pancreatic cancer, investigators uncover genetic evidence for the role of STAT3 signaling in cancer-associated fibroblasts in promoting tumor progression and an immunosuppressive microenvironment.
CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7The structure of mammalian V-ATPase with mEAK-7 shows how a TLDc domain-containing protein can bind the proton pump to form an activity-sensitive interaction.
Analyzing efficacy, stability, and safety of AAV-mediated optogenetic hearing restoration in miceThis longitudinal study revealed stable expression of the fast-gating channelrhodopsin f-Chrimson in cochlear neurons of mice over at least 2 yr upon a single postnatal AAV dosing of the cochlea.
Proteins implicated in muscular dystrophy and cancer are functional constituents of the centrosomeThis study demonstrates that the muscular dystrophy-associated proteins dystrophin, utrophin, dysferlin, and calpain-3 localize to the centrosome and that their absence leads to excess centrosomes, compromised nuclear morphology, impaired centrosome orientation, and defective microtubule nucleation.
Drosophila insulator proteins exhibit in vivo liquid–liquid phase separation propertiesDrosophila insulator proteins and the cohesin subunit Rad21 coalesce in vivo to form liquid-droplet condensates, suggesting that liquid–liquid phase separation mediates their function in 3D genome organization.