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CRISPR-induced double-strand breaks trigger recombination between homologous chromosome armsCRISPR–Cas9 enables recombination between homologous chromosome arms at predefined sites and also underscores the need for caution when applying CRISPR technologies in translational medicine.
Detecting sequence signals in targeting peptides using deep learningDuring the development of TargetP 2.0, a state-of-the-art method to predict targeting signal, we find a previously overlooked biological signal for subcellular targeting using the output from a deep learning method.
A network of human functional gene interactions from knockout fitness screens in cancer cellsThe function of human genes can be strongly inferred from their knockout fitness profiles across hundreds of CRISPR screens, illuminating the modular organization of the cell.
m6A-mRNA methylation regulates cardiac gene expression and cellular growthThis study provides evidence that m6A methylation is dynamically regulated during human and murine cardiac disease and highlights an important role of the m6A methylase Mettl3 in regulating cardiac growth by gene expression control.
Mesenchymal stromal cell activation by breast cancer secretomes in bioengineered 3D microenvironmentsThis study shows the activation of tumour-associated mesenchymal stromal cells by breast cancer secretomes in bioengineered 3D microenvironments using comprehensive multiomics analysis methods.
Metabolic reprogramming of fibro/adipogenic progenitors facilitates muscle regenerationHigh-fat diet ameliorates muscle dystrophic phenotype by promoting the FAP-dependent myogenesis of satellite cells.
EBV renders B cells susceptible to HIV-1 in humanized miceA proportion of EBV+ B cells are infectible by CXCR4-tropic HIV-1 in vitro and in vivo, and these HIV-infected B cells can transmit infection to previously virus-naïve humanized mice.
Reciprocal regulation of STING and TCR signaling by mTORC1 for T-cell activation and functionCostimulation of T cells through both TCR and STING induces growth inhibition by partially blocking the mTORC1 signals, and leads to IFN-I production through sustained activation of IRF3 and mTORC1 activation.
Optimized ChIP-seq method facilitates transcription factor profiling in human tumorsThis study presents an optimized ChIP-seq protocol to enhance transcription factor profiling in human tumours, enabling the analysis of highly challenging samples, including core needle biopsies.
Virus-induced transposable element expression up-regulation in human and mouse host cellsGenome-wide transposon expression up-regulation in host cells regardless of virus, species, and host cell tissue types occurs early during viral infection and likely contributes to promoting the host innate immune response.