Most read article(s)
Targeting TREM2 signaling shows limited impact on cerebrovascular calcificationMicroglia protect the brain from calcification and require the TREM2 receptor for this role. Here, we find that boosting TREM2 function using an antibody increases a TREM2-dependent response of microglia to vascular calcifications but is insufficient to resolve the pathology.
Twisting the theory on the origin of human umbilical cord coiling featuring monozygotic twinsThis study provides a novel framework to understand the molecular origins of human umbilical cord helices using state-of-the-art imaging and omics approaches.
Detecting sequence signals in targeting peptides using deep learningDuring the development of TargetP 2.0, a state-of-the-art method to predict targeting signal, we find a previously overlooked biological signal for subcellular targeting using the output from a deep learning method.
ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose toleranceciBAR1-KO mice exhibit ciliopathy phenotypes such as embryonic lethality and exocrine and endocrine pancreatic defects, indicating that ciBAR1 plays a critical role in ciliogenesis in vivo.
Mechanical compressive forces increase PI3K output signaling in breast and pancreatic cancer cellsThis article provides a proof-of-concept showing that the therapeutic impact of targeted therapies on solid cancer cells is dependent on compressive mechanical context.
Depleting chemoresponsive mitochondrial fission mediator DRP1 does not mitigate sarcoma resistanceThe mitochondrial fission mediator DRP1 levels and activation are modulated upon chemotherapy exposure, yet depleting DRP1 does not restore chemosensitivity in the most common pediatric sarcomas.
FrozONE: quick cell nucleus enrichment for comprehensive proteomics analysis of frozen tissuesThe authors establish and benchmark a nuclear proteomics workflow that is faster, simpler, and more scalable than classical protocols, while being as efficient, specific, and reproducible.
Vnn1 pantetheinase limits the Warburg effect and sarcoma growth by rescuing mitochondrial activityExpression of the Vnn1 pantetheinase by sarcomas is tumor suppressive by limiting the use of aerobic glycolysis for growth and rescuing mitochondrial activity through CoA regeneration.
Activation of the Keap1/Nrf2 pathway suppresses mitochondrial dysfunction, oxidative stress, and motor phenotypes in C9orf72 ALS/FTD modelsC9orf72 mutations cause multiple mitochondrial disruptions, but only genetically boosting antioxidant expression rescued motor deficits in vivo, supporting a pathogenic connection. Genetic or pharmacological activation of the Keap1/Nrf2 pathway ameliorated pathological phenotypes in Drosophila models and patient-derived iNeurons.
Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic KrasG12D in pancreatic cancerThis work identifies the use of exosomes to specifically deliver CRISPR/Cas9 to target oncogenic KrasG12D mutation in pancreatic cancer as a nonviral therapeutic strategy.